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Recombinant Escherichia coli 30S ribosomal protein S5 (rpsE)

Recombinant Escherichia coli 30S ribosomal protein S5 (rpsE)

Code	CSB-YP364073ENV
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP364073ENV
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP364073ENV-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP364073ENV
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP364073ENV
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

rpsE

Uniprot No.

P0A7W1

Alternative Names

rpsE; spc; b3303; JW3265; 30S ribosomal protein S5; Small ribosomal subunit protein uS5

Species

Escherichia coli (strain K12)

Expression Region

2-167

Target Protein Sequence

AHIEKQAGE LQEKLIAVNR VSKTVKGGRI FSFTALTVVG DGNGRVGFGY GKAREVPAAI QKAMEKARRN MINVALNNGT LQHPVKGVHT GSRVFMQPAS EGTGIIAGGA MRAVLEVAGV HNVLAKAYGS TNPINVVRAT IDGLENMNSP EMVAAKRGKS VEEILGK

Protein Length

Full Length of Mature Protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

With S4 and S12 plays an important role in translational accuracy. Many suppressors of streptomycin-dependent mutants of protein S12 are found in this protein, some but not all of which decrease translational accuracy (ram, ribosomal ambiguity mutations).; Located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body.; The physical location of this protein suggests it may also play a role in mRNA unwinding by the ribosome, possibly by forming part of a processivity clamp.

Gene References into Functions

The role of S5 is in coordinating the 5' end maturation and the 3' domain assembly. PMID: 24671761
the importance of rpsE in ribosome function and cell physiology PMID: 17053085
rimJ as a high-copy suppressor of the cold-sensitive phenotype associated with the S5(G28D) mutation. PMID: 18466225

Protein Families

Universal ribosomal protein uS5 family

Database Links

KEGG: ecj:JW3265

STRING: 316385.ECDH10B_3478

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