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Recombinant Escherichia coli Aerobic respiration control sensor protein ArcB (arcB), partial

Recombinant Escherichia coli Aerobic respiration control sensor protein ArcB (arcB), partial

Code	CSB-YP365123ENV1
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP365123ENV1
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP365123ENV1-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP365123ENV1
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP365123ENV1
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

arcB

Uniprot No.

P0AEC3

Alternative Names

arcB; b3210; JW5536; Aerobic respiration control sensor protein ArcB

Species

Escherichia coli (strain K12)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Member of the two-component regulatory system ArcB/ArcA. Sensor-regulator protein for anaerobic repression of the arc modulon. Activates ArcA via a four-step phosphorelay. ArcB can also dephosphorylate ArcA by a reverse phosphorelay involving His-717 and Asp-576.

Gene References into Functions

The authors observed that wild-type, DeltaarcA, and DeltaarcB had comparable initial NO* clearance times; however, the mutant cultures failed to exhibit [NO*] and [O2 ] oscillations. these results establish ArcAB as a TCS that influences NO* detoxification in E. coli within the physiologically-relevant microaerobic regime. PMID: 28449226
Data show that the redox potential of ArcB is about -41 mV, and we demonstrate that the menaquinols are required for proper ArcB activation upon a shift from aerobic to anaerobic growth conditions. PMID: 23645604
These analyses revealed that elimination of different segments in ArcB determines a distinctive distribution of d-glucose catabolic fluxes, different from that observed in the DeltaarcB background. PMID: 23064346
The results lead to the working hypothesis that the in vivo activity of ArcB in E. coli is modulated by the redox state of the menaquinone pool and that the ubiquinone/ubiquinol ratio in vivo surely is not the only determinant of ArcB activity. PMID: 19933363
the ArcB/ArcA/RssB system is involved in sigma(S) induction during entry into starvation conditions. PMID: 16291649
An active form of ArcB promotes low-level induction of psp expression independent of pIV and communicates with PspB. PMID: 19682256
Both ArcA and ArcB were necessary for resistance to hydrogen peroxide, and their function in this resistance was independent from H2O2 scavenge. PMID: 19715602