Product Details

Purity

>85% (SDS-PAGE)

Target Names

flu

Uniprot No.

P39180

Alternative Names

flu; yeeQ; yzzX; b2000; JW1982Antigen 43; AG43; Fluffing protein) [Cleaved into: Antigen 43 alpha chain; Antigen 43 beta chain]

Species

Escherichia coli (strain K12)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Controls colony form variation and autoaggregation. May function as an adhesin.

Gene References into Functions

In the K-12 leader sequence, two in-frame translation initiation codons have been identified, one upstream and the other downstream of the transcription terminator. For optimal agn43 expression, both codons need to be present. PMID: 24837285
Lrp and MqsR, previously identified as agn43 regulators, do not regulate agn43 expression or ON/OFF switch frequency. PMID: 24039985
The data support a model in which in an ON-phase cell, the subcellular OxyR availability at the replication fork as it passes through the agn43 regulatory region is key for initiating an ON-to-OFF switch. PMID: 20118257
glycosylated form of Ag43 enhances bacterial binding to human cell lines PMID: 16484190
In vivo, the H107A substitution in sigma(32) impedes recovery from heat shock (exposure to 42 degrees C), and it also leads to overexpression at lower temperatures (30 degrees C) of the Flu protein, which is associated with biofilm formation PMID: 17921304
that glycosylation may play significant roles in structural and functional properties of bacterial autotransporters Ag43 at many different levels. PMID: 18341480
Proposed roles of Ag43 expression are summarized and the studies are put into perspective regarding the role of allelic variants, genetic background of the bacterial strain, and control of expression by phase variation. Review. PMID: 18785838
The authors show that Ag43 mediates bacterial adhesion in a cell line-specific manner and that structural variations of the alpha(43) domain correlate with increased adhesive properties to proteins of the extracellular matrix such as collagen and laminin. PMID: 19230760

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Subcellular Location

[Antigen 43]: Periplasm.; [Antigen 43 alpha chain]: Secreted. Cell surface. Note=The cell surface component is about 60 kDa and can be released by mild heat treatment (PubMed:22466966).; [Antigen 43 beta chain]: Cell outer membrane; Multi-pass membrane protein. Note=May form a beta-barrel.

Database Links

KEGG: ecj:JW1982

STRING: 316385.ECDH10B_2146

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Recombinant Escherichia coli Antigen 43 (flu), partial

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