Recombinant Escherichia coli Maltose regulon regulatory protein malI(malI)

Code CSB-YP322474ENV
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Source Yeast
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Code CSB-EP322474ENV
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Source E.coli
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Code CSB-EP322474ENV-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP322474ENV
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Source Baculovirus
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Code CSB-MP322474ENV
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names malI
Uniprot No. P18811
Alternative Names malI; b1620; JW1612Maltose regulon regulatory protein MalI
Species Escherichia coli (strain K12)
Expression Region 1-342
Target Protein Sequence MATAKKITIH DVALAAGVSV STVSLVLSGK GRISTATGER VNAAIEELGF VRNRQASALR GGQSGVIGLI VRDLSAPFYA ELTAGLTEAL EAQGRMVFLL HGGKDGEQLA QRFSLLLNQG VDGVVIAGAA GSSDDLRRMA EEKAIPVIFA SRASYLDDVD TVRPDNMQAA QLLTEHLIRN GHQRIAWLGG QSSSLTRAER VGGYCATLLK FGLPFHSDWV LECTSSQKQA AEAITALLRH NPTISAVVCY NETIAMGAWF GLLKAGRQSG ESGVDRYFEQ QVSLAAFTDA TPTTLDDIPV TWASTPAREL GITLADRMMQ KITHEETHSR NLIIPARLIA AK
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Repressor for the malX and malY genes. Also regulates its own expression. Binds maltose as an inducer.
Gene References into Functions
  1. The malI promoter is repressed by MalI binding from position +3 to position +18, downstream of the malI transcript start. PMID: 20141531
  2. The malI promoter drives transcription initiation from a location 85 bp upstream from the malX transcript start and it is active without the CRP. Expression from the malI promoter can be stimulated by the CRP. PMID: 19054084
Database Links

KEGG: ecj:JW1612

STRING: 316385.ECDH10B_1753

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