Recombinant Escherichia coli Protease 2 (ptrB)

Code CSB-YP340632ENV
MSDS
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Source Yeast
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Code CSB-EP340632ENV
MSDS
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Source E.coli
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Code CSB-EP340632ENV-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP340632ENV
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP340632ENV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ptrB
Uniprot No.
Alternative Names
ptrB; tlp; b1845; JW1834; Protease 2; EC 3.4.21.83; Oligopeptidase B; Protease II
Species
Escherichia coli (strain K12)
Expression Region
1-686
Target Protein Sequence
MLPKAARIPH AMTLHGDTRI DNYYWLRDDT RSQPEVLDYL QQENSYGHRV MASQQALQDR ILKEIIDRIP QREVSAPYIK NGYRYRHIYE PGCEYAIYQR QSAFSEEWDE WETLLDANKR AAHSEFYSMG GMAITPDNTI MALAEDFLSR RQYGIRFRNL ETGNWYPELL DNVEPSFVWA NDSWIFYYVR KHPVTLLPYQ VWRHAIGTPA SQDKLIYEEK DDTYYVSLHK TTSKHYVVIH LASATTSEVR LLDAEMADAE PFVFLPRRKD HEYSLDHYQH RFYLRSNRHG KNFGLYRTRM RDEQQWEELI PPRENIMLEG FTLFTDWLVV EERQRGLTSL RQINRKTREV IGIAFDDPAY VTWIAYNPEP ETARLRYGYS SMTTPDTLFE LDMDTGERRV LKQTEVPGFY AANYRSEHLW IVARDGVEVP VSLVYHRKHF RKGHNPLLVY GYGSYGASID ADFSFSRLSL LDRGFVYAIV HVRGGGELGQ QWYEDGKFLK KKNTFNDYLD ACDALLKLGY GSPSLCYAMG GSAGGMLMGV AINQRPELFH GVIAQVPFVD VVTTMLDESI PLTTGEFEEW GNPQDPQYYE YMKSYSPYDN VTAQAYPHLL VTTGLHDSQV QYWEPAKWVA KLRELKTDDH LLLLCTDMDS GHGGKSGRFK SYEGVAMEYA FLVALAQGTL PATPAD
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Cleaves peptide bonds on the C-terminal side of lysyl and argininyl residues.
Gene References into Functions
  1. results suggest that translation initiation of ptrB mRNA is controlled via its 5'-terminal AUG which, in cooperation with the ptrB 5'-UTR, represents a novel form of regulation that can act as an autonomous control element independent of gene context PMID: 28484048
  2. Two consecutive basic residues are a preferred cleavage site for the peptidase. PMID: 24225368
  3. Site-directed mutagenesis of staphylokinase(SAK) His43 and SAKTyr44 demonstrated the crucial requirement of a positively charged and an aromatic residue, respectively, at these positions for optimal functioning of SAK-plasmin activator complex. PMID: 21510941
Protein Families
Peptidase S9A family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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