Recombinant Escherichia coli Uncharacterized oxidoreductase yjgI (yjgI)

Code CSB-YP336558ENV
MSDS
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Source Yeast
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Code CSB-EP336558ENV
MSDS
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Source E.coli
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Code CSB-EP336558ENV-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP336558ENV
MSDS
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Source Baculovirus
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Code CSB-MP336558ENV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
bdcA
Uniprot No.
Alternative Names
bdcA; yjgI; b4249; JW4207Cyclic-di-GMP-binding biofilm dispersal mediator protein
Species
Escherichia coli (strain K12)
Expression Region
1-237
Target Protein Sequence
MGAFTGKTVL ILGGSRGIGA AIVRRFVTDG ANVRFTYAGS KDAAKRLAQE TGATAVFTDS ADRDAVIDVV RKSGALDILV VNAGIGVFGE ALELNADDID RLFKINIHAP YHASVEAARQ MPEGGRILII GSVNGDRMPV AGMAAYAASK SALQGMARGL ARDFGPRGIT INVVQPGPID TDANPANGPM RDMLHSLMAI KRHGQPEEVA GMVAWLAGPE ASFVTGAMHT IDGAFGA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Increases biofilm dispersal. Acts by binding directly to the signaling molecule cyclic-di-GMP, which decreases the intracellular concentration of cyclic-di-GMP and leads to biofilm dispersal. Also controls other biofilm-related phenotypes such as cell motility, cell size, cell aggregation and production of extracellular DNA and extracellular polysaccharides (EPS). Does not act as a phosphodiesterase.
Gene References into Functions
  1. This study shows the structure of BdcA in complex with NADPH and confirms that NADPH binding is requisite for BdcA facilitating cell motility and increasing biofilm dispersal. PMID: 29462616
Protein Families
Short-chain dehydrogenases/reductases (SDR) family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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