Recombinant Escherichia coli Uncharacterized protein ygiF(ygiF)

Code CSB-YP341185ENV
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Source Yeast
Code CSB-EP341185ENV
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Source E.coli
Code CSB-EP341185ENV-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Code CSB-BP341185ENV
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Source Baculovirus
Code CSB-MP341185ENV
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Source Mammalian cell

Product Details

Purity >85% (SDS-PAGE)
Target Names ygiF
Uniprot No. P30871
Species Escherichia coli (strain K12)
Expression Region 1-433
Target Protein Sequence MAQEIELKFI VNHSAVEALR DHLNTLGGEH HDPVQLLNIY YETPDNWLRG HDMGLRIRGE NGRYEMTMKV AGRVTGGLHQ RPEYNVALSE PTLDLAQLPT EVWPNGELPA DLASRVQPLF STDFYREKWL VAVDGSQIEI ALDQGEVKAG EFAEPICELE LELLSGDTRA VLKLANQLVS QTGLRQGSLS KAARGYHLAQ GNPAREIKPT TILHVAAKAD VEQGLEAALE LALAQWQYHE ELWVRGNDAA KEQVLAAISL VRHTLMLFGG IVPRKASTHL RDLLTQCEAT IASAVSAVTA VYSTETAMAK LALTEWLVSK AWQPFLDAKA QGKISDSFKR FADIHLSRHA AELKSVFCQP LGDRYRDQLP RLTRDIDSIL LLAGYYDPVV AQAWLENWQG LHHAIATGQR IEIEHFRNEA NNQEPFWLHS GKR
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.
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Target Data

Function Involved in the hydrolysis of the beta-gamma-phosphoanhydride linkage of triphosphate-containing substrates (inorganic or nucleoside-linked). Catalyzes the hydrolysis of inorganic triphosphate (PPPi), which could be cytotoxic because of its high affinity for calcium ion, thereby interfering with calcium signaling. It also hydrolyzes slowly thiamine triphosphate (ThTP). YgiF is a specific PPPase, but it contributes only marginally to the total PPPase activity in E.coli, where the main enzyme responsible for hydrolysis of PPPi is inorganic pyrophosphatase (PPase).
Database Links

KEGG: ecj:JW3026

STRING: 316385.ECDH10B_3229

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