Recombinant European bat lyssavirus 2 RNA-directed RNA polymerase L(L) ,partial

Code CSB-YP395885EJI
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Source Yeast
Code CSB-EP395885EJI
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Source E.coli
Code CSB-EP395885EJI-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Code CSB-BP395885EJI
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Source Baculovirus
Code CSB-MP395885EJI
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Source Mammalian cell

Product Details

Purity >85% (SDS-PAGE)
Target Names L
Uniprot No. A4UHQ7
Species European bat lyssavirus 2 (strain Human/Scotland/RV1333/2002) (EBLV2)
Expression Region 1-2127
Target Protein Sequence MIDPLEVYDD PVDPVEPEID ARSNSVVPNI LRNSDYNLNS PLIEDPSKLM LDWLITGNKP ARLNLTDNSL RSYKVLKSYF KKLDVGSLRV GGLGAQSMMS LWLHGAHSES TRSRKCLSDL ALFYQRSAPI EKLLNYTLEN RGLAIPTDGV LSSLKKVNYD RAFGRYLGNL YSSYLFFHVI ILYMNALDWE EEKTILALWK DLNSVDIKKD QVKFRDQIWG SLLVTKDFVY SQSANCLFDR NYTLMLKDLF LSRFNSLLIL LSPPEPRYSE DLISQLCQLY IAGDNVLSTC GNSGYDVIKM LEPYVVNSLV QRAEGFRPMI HSLGDFPTFI KDKVSQLEGT FGPSARNFFF VLDQLDNIHD LVFVYGCYRH WGHPYIDYRK GLSKLYDQVH VKKVIDGDYQ ECLASDLAKR ILRWGFDKYS KWYLDPKLLE KDHPLIPYIQ TQTWPPKHIV DIVGNTWHKL PITQIFEIPE SMDPSEILDD KSHSFTRTKL ASWLSDHRGG PVPSEKVIIT ALSRPPVNPR EFLKSIDLGG LPDDDLIIGL KPKERELKIE GRFFALMSWN LRLYFVITEK LLANYILPLF DALTMTDNLN KVFKKLIDRV TGQGLQDYSR VTYAFHLDYE KWNNHQRLES TKDVFSVLDY VFGLKKVFSR THEFFQKSWV YYSDRSDLIG LWEDQIYCLD MSDGPTCWNG QDGGLEGLRQ KGWSLVSLLM IDRESQTRNT RTKILAQGDN QVLCPTYMLS SGLTQEGLIY ELDSISRNAL SIYRAIEEGA SKLGLIIKKE ETMCSYDFLI YGKTPLFRGN ILVPESKRWA RVSCISNDQI VNLANIMSTV STNALTVAQH SQSLIKPMRD FLLMAVQAVF HYLLFSPILK DRVYKILSAE GDNFLLAMSR IIYLDPSLGG VSGMSLGRFH IRQFSDPVSE GLAFWKEIWS SSSESWIHSL CQEAGNPDLG DRSLESFTRL LEDPTTLNIR GGASPTILLK EAIRKALYDE VDKVENSEFR EAILLSKTHR DNFILFLKSI EPLFPRFLSE LFSSSFLGIP ESIIGLIQNS RTIRRQFRRS LSRTLEESFF NSEIHGINRM TQVPQRVGRV WNCSAERADL LREISWGRKV VGTTVPHPGE MLVLLPKSSV SCVCKQTGED SPRISVSVLP SFDQSFFSRG PLKGYLGSST SMSTQLFHAW EKVTNVHVVK RALSLKESIN WFIARDSNLA QTLIRNITSL TGPQFPLEET PVFKRTGSAL HRFKSARYSE GGYSSICPNL LSHISVSTDT MSDLTQDGKN FDFMFQPLML YAQTWTSELV QKDIRLRDST FHWHLRCLKC IRPIDDIILD APQVFMFPDV SKRISRMVSG AVPQFQRLPE INLKPGKFEA LDSKDKSRHI GTAQGLLYSI LVAIHDSGYN DATIFPMNIY SKISPRDYLR GLSRGILIGS SICFLTRMTN ININRPLELI SGVISYILLR LDNHPSLYVM LREPSLRSEI FSIPQKIPAA YPTTMKEGNR SVLCYLQHVL RYERDVITAS PENDWLWIFS DFRSAKMTYL TLVTYQSHIL LQKIEKNLPK QMRIRLRQLS SLMRQILGGH GEDTLDSDED IQGLLRDALQ RTRWVDQEVR HAAKTMTGDH SPSKKVSRKA GCSEWICSAQ QVAISTSSNP APTSEMDVRA LSRRFQNPLI SGLRVVQWAT GAHYKLKPIL DNLEAYPSLC LVVGDGSGGI SRTVLSMFPD AKLVFNSLLE VNDFMASGTH PLPPSAIVSG GDDIVSRVID FGSIWEKPSD LRNLSTWRYF QSIQTVNNMS YDLIVCDAEV TDIPSVNKIT LLMSDFSLSI NGPLNLIFKT YGTMLVNPDY KAIQHLSRAF PSVTGYITQM TSSFSSELYL KFSKRGKFFR DAEYLTSSTL REMSLVLFNC SSPKSEMQRA RSLNYQDLVR GFPEEIISNP YNEMIITLID SEVESFLVHK MVDDLELQRG TLAKMSIIIA IVMVFSNRVF NVSKPLTDPM FYPPSDPKIL RHFNICCSTL MYLSTALGDV LNFARLHELY NNPITYYFKK QVIRGSIYLS WSWCDTTSVF KKVACNSNLS LSSHWIRLIY KIVRTTRLAG SSNDLSKEVE KHLKGYNRWI SFDDIRSRSS LLDYSCL
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.
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Target Data

Function RNA-directed RNA polymerase that catalyzes the transcription of viral mRNAs, their capping and polyadenylation. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The viral polymerase binds to the genomic RNA at the 3' leader promoter, and transcribes subsequently all viral mRNAs with a decreasing efficiency. The first gene is the most transcribed, and the last the least transcribed. The viral phosphoprotein acts as a processivity factor. Capping is concommitant with initiation of mRNA transcription. Indeed, a GDP polyribonucleotidyl transferase (PRNTase) adds the cap structure when the nascent RNA chain length has reached few nucleotides. Ribose 2'-O methylation of viral mRNA cap precedes and facilitates subsequent guanine-N-7 methylation, both acticities being carried by the viral polymerase. Polyadenylation of mRNAs occur by a stuttering mechanism at a slipery stop site present at the end viral genes. After finishing transcription of a mRNA, the polymerase can resume transcription of the downstream gene.
Subcellular Location Virion, Host cytoplasm
Protein Families Rhabdoviridae protein L family
Database Links

KEGG: vg:5219912

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