Recombinant Fruit fly Accessory gland-specific peptide 70A (SP)

Code CSB-YP356570DLU
MSDS
Size $472
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
SP
Uniprot No.
Research Area
Others
Alternative Names
SP; Acp70A; PAPB; CG17673Accessory gland-specific peptide 70A; Paragonial peptide B; Sex peptide; SP
Species
Drosophila melanogaster (Fruit fly)
Source
Yeast
Expression Region
20-55aa
Target Protein Sequence
WEWPWNRKPTKFPIPSPNPRDKWCRLNLGPAWGGRC
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
4.3kDa
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Male seminal protein which triggers short- and long-term post-mating behavioral responses (PMR) in female Drosophila. Binds initially to sperm where it is later cleaved to release an active peptide within the female reproductive tract. Signals via the sex peptide receptor (SPR) in female flies; may also act via other receptors. Moderates the activity of distinct neuronal circuitries in the female genital tract to promote specific PMRs including: enhanced ovulation, increased egg laying rate, increased feeding/foraging rate, induced antimicrobial peptide synthesis, reduced mating receptivity, reduced day-time sleep and reduced lifespan in multiple mated females.
Gene References into Functions
  1. Molecular Functions and Cross-Species Activity of Sex-Peptide in Drosophila PMID: 26022240
  2. sex peptide is required for normal magnitude and persistence of postmating responses PMID: 12893873
  3. sex peptide is the major agent eliciting the short-term and the long-term postmating responses PMID: 12897240
  4. Drosophila sex-peptide stimulates female innate immune system after mating via the Toll and Imd pathways. PMID: 16169493
  5. Copulation strongly stimulates female food intake and Sex Peptide is the main agent controlling this behavioral paradigm. PMID: 16581515
  6. SP acts by chemical mimicry of sugar components of the bacterial cell wall. Thus, SP may induce the immune system via pattern recognition receptors. PMID: 17922838
  7. Data show significant variation in expression levels of the SP gene Acp70A across D. melanogaster isolines. PMID: 19640319

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Subcellular Location
Secreted.
Protein Families
Drosophila sex peptide family
Tissue Specificity
Main cells of the accessory glands of males (paragonial gland).
Database Links

KEGG: dme:Dmel_CG17673

STRING: 7227.FBpp0075572

UniGene: Dm.2898

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