Recombinant Geobacillus thermodenitrificans UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE)

Code CSB-YP392034GEH
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Source Yeast
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Code CSB-EP392034GEH
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Source E.coli
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Code CSB-EP392034GEH-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP392034GEH
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Source Baculovirus
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Code CSB-MP392034GEH
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
murE
Uniprot No.
Alternative Names
murE; GTNG_0980; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase; EC 6.3.2.13; Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase
Species
Geobacillus thermodenitrificans (strain NG80-2)
Expression Region
1-489
Target Protein Sequence
MKLQTLLSRL PGFWVHRGGN PDIVALEMDS RHVTPGSLFF CVKGFTVDGH DFAEQAVERG AVAIVAERPL SVNVPVVVVP DSRRAMAILA DAFYGQPTHR LHLIGVTGTN GKTTTTHIIE QVARKAGKKI GLIGTVGTKI GDRSYPAVNT TPESLVLQRT FKQMVDEGVE FVTMEVSSHA LHQGRVHGCD YDVAVFTNLT QDHLDYHGTM EEYRNAKGLL FAQLGNRYDE RRPKFAVLNH DDPVSQYYKH MTAAPIITYG IKEKSDVMAE QIEMTPGGMA FQLCTPHGTM AIETKLVGLF NVYNLLAATA ACLASGFSLA TIAEALANVS PVPGRFETVD EGQNFTVIVD YAHTPDSVEN ALKTVRQFAK RNVYVVIGCG GDRDRTKRPL MAQAAVRYAD VAVFTSDNPR SEDPRQILRD MEAGVSAGDG TYVTIPDREE AIRYAIGQAQ EGDVVLIAGK GHETYQIIGD DVIDFDDRAV ARAAVKERR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.
Subcellular Location
Cytoplasm.
Protein Families
MurCDEF family, MurE subfamily
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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