Recombinant Gibberella zeae Protein RAI1 (RAI1)

Code CSB-YP676851GGB
MSDS
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Source Yeast
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Code CSB-EP676851GGB
MSDS
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Source E.coli
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Code CSB-EP676851GGB-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP676851GGB
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP676851GGB
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RAI1
Uniprot No.
Alternative Names
RAI1; FGRRES_16819; FGSG_07345; FGSG_13131Decapping nuclease RAI1; EC 3.6.1.-
Species
Gibberella zeae (strain PH-1 / ATCC MYA-4620 / FGSC 9075 / NRRL 31084) (Wheat head blight fungus) (Fusarium graminearum)
Expression Region
2-368
Target Protein Sequence
AARFSIQPI GRFAGESQPV KRPKEFACFS YDDNHEFRLD GSSLKYYYTP QLGADLSKGF DTFQKLDDTG DDHLDSLLKT IVAHEQETGK KIDANVVTWR GMMTKIMATP FDNMDGFEMN ATLYQNNAYK VASRSNEGNN NRRRRGPPLE VMQFWGYKFE TLSTLPAPWA ETPREFIENR ENEVVNNKAQ YCSVVRTGIG KSVLCLGGEV DAIWDSKPEE KGSPINWVEL KTSAEIRNTG DMENFNRKLM KYWIQSFLLG VPRIVVGFRT RDGILVEAKD IETHRIPETV NSYPNPKWNA DMCVNFAATF LDWLSANITD EGVWRIKREP QSPTIELFKV EETGHGDILS DEFKNWRIKL ALGPSNES
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Decapping enzyme for NAD-capped RNAs: specifically hydrolyzes the nicotinamide adenine dinucleotide (NAD) cap from a subset of RNAs by removing the entire NAD moiety from the 5'-end of an NAD-capped RNA. The NAD-cap is present at the 5'-end of some RNAs and snoRNAs. In contrast to the canonical 5'-end N7 methylguanosine (m7G) cap, the NAD cap promotes mRNA decay. Also acts as a non-canonical decapping enzyme that removes the entire cap structure of m7G capped or incompletely capped RNAs. Has decapping activity toward incomplete 5'-end m7G cap mRNAs such as unmethylated 5'-end-capped RNA (cap0), while it has no activity toward 2'-O-ribose methylated m7G cap (cap1).
Subcellular Location
Nucleus.
Protein Families
DXO/Dom3Z family
Database Links
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