Recombinant Helicobacter pylori Oxygen-insensitive NADPH nitroreductase (rdxA)

Code CSB-YP519611HUV
MSDS
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Source Yeast
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Code CSB-EP519611HUV
MSDS
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Source E.coli
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Code CSB-EP519611HUV-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP519611HUV
MSDS
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Source Baculovirus
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Code CSB-MP519611HUV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
rdxA
Uniprot No.
Alternative Names
rdxA; HP_0954; Oxygen-insensitive NADPH nitroreductase; EC 1.-.-.-
Species
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Expression Region
1-210
Target Protein Sequence
MKFLDQEKRR QLLNERHSCK MFDSHYEFSS TELEEIAEIA RLSPSSYNTQ PWHFVMVTDK DLKKQIAAHS YFNEEMIKSA SALMVVCSLR PSELLPHGHY MQNLYPESYK VRVIPSFAQM LGVRFNHSMQ RLESYILEQC YIAVGQICMG VSLMGLDSCI IGGFDPLKVG EVLEERINKP KIACLIALGK RVAEASQKSR KSKVDAITWL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Reduction of a variety of nitroaromatic compounds using NADPH as source of reducing equivalents; two electrons are transferred. Capable of reducing metronidazole; inactive RdxA renders the bacterium resistant to this compound. The reduction of metronidazole generates hydroxylamine, a potent mutagen and bactericide.
Gene References into Functions
  1. Its mutation is a cause of metronidazole resistance in Helicobacter pylori. PMID: 28748532
  2. Analysis of allelic variants of oxygen-insensitive NADPH nitroreductase (RdxA) associated with metronidazole resistance in Helicobacter pylori has been reported. PMID: 27706703
Protein Families
Nitroreductase family
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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