Recombinant Histone H3-like centromeric protein hcp-3 (hcp-3)

Code CSB-YP335943CXY
MSDS
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Source Yeast
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Code CSB-EP335943CXY
MSDS
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Source E.coli
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Code CSB-EP335943CXY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP335943CXY
MSDS
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Source Baculovirus
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Code CSB-MP335943CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
hcp-3
Uniprot No.
Alternative Names
hcp-3; F58A4.3; Histone H3-like centromeric protein hcp-3; CENP-A homolog; CeCENP-A; Holocentric chromosome-binding protein 3
Species
Caenorhabditis elegans
Expression Region
1-288
Target Protein Sequence
MADDTPIIEE IAEQNESVTR IMQRLKHDMQ RVTSVPGFNT SAAGVNDLID ILNQYKKELE DDAANDYTEA HIHKIRLVTG KRNQYVLKLK QAEDEYHARK EQARRRASSM DFTVGRNSTN LVDYSHGRHH MPSYRRHDSS DEENYSMDGT NGDGNRAGPS NPDRGNRTGP SSSDRVRMRA GRNRVTKTRR YRPGQKALEE IRKYQKTEDL LIQKAPFARL VREIMQTSTP FGADCRIRSD AISALQEAAE AFLVEMFEGS SLISTHAKRV TLMTTDIQLY RRLCLRHL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division. Might promote cleavage furrow stability during cytokinesis. Not required for chromosome segregation during meiosis.
Gene References into Functions
  1. Results suggest that acetylation of soluble H4 may stimulate the initial deposition of CENP-A(HCP-3)-H4 nucleosomes. PMID: 29653589
Subcellular Location
Nucleus. Chromosome, centromere. Chromosome, centromere, kinetochore.
Protein Families
Histone H3 family
Database Links

KEGG: cel:CELE_F58A4.3

STRING: 6239.F58A4.3

UniGene: Cel.10383

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