Recombinant Human Aldo-keto reductase family 1 member B15 (AKR1B15)

Code CSB-YP513924HU(F2)
MSDS
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Source Yeast
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Code CSB-EP513924HU
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Source E.coli
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Code CSB-EP513924HU(F2)-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP513924HU
MSDS
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Source Baculovirus
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Code CSB-MP513924HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
AKR1B15
Uniprot No.
Alternative Names
AKR1B15Aldo-keto reductase family 1 member B15; EC 1.1.1.-; EC 1.1.1.300; EC 1.1.1.54; Estradiol 17-beta-dehydrogenase AKR1B15; Farnesol dehydrogenase; EC 1.1.1.216; Testosterone 17beta-dehydrogenase; EC 1.1.1.64
Species
Homo sapiens (Human)
Expression Region
1-344
Target Protein Sequence
MVLQMEPQVN STNNFHQGPL DQPVGPLTGL KSSLLKDTTS AGPLLRPYPA SLLGKVKEAV KVAIDAEYRH IDCAYFYENQ HEVGEAIQEK IQEKAVMRED LFIVSKVWPT FFERPLVRKA FEKTLKDLKL SYLDVYLIHW PQGFKTGDDF FPKDDKGNMI SGKGTFLDAW EAMEELVDEG LVKALGVSNF NHFQIERLLN KPGLKYKPVT NQVECHPYLT QEKLIQYCHS KGITVTAYSP LGSPDRPWAK PEDPSLLEDP KIKEIAAKHK KTTAQVLIRF HIQRNVTVIP KSMTPAHIVE NIQVFDFKLS DEEMATILSF NRNWRAFDFK EFSHLEDFPF DAEY
Protein Length
Full Length of Isoform 2
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalyzes the NADPH-dependent reduction of a variety of carbonyl substrates, like aromatic aldehydes, alkenals, ketones and alpha-dicarbonyl compounds. In addition, catalyzes the reduction of androgens and estrogens with high positional selectivity (shows 17-beta-hydroxysteroid dehydrogenase activity) as well as 3-keto-acyl-CoAs. Displays strong enzymatic activity toward all-trans-retinal and 9-cis-retinal. May play a physiological role in retinoid metabolism.; No oxidoreductase activity observed with the tested substrates.
Gene References into Functions
  1. Amino acid substitutions clustered in loops A and C result in a smaller more hydrophobic and more rigid active site in AKR1B15 compared with the AKR1B10 pocket, consistent with distinct substrate specificity and narrower inhibitor selectivity for AKR1B15. PMID: 26222439
  2. AKR1B15.2 localizes to the cytosol and displays no enzymatic activity with the substrates tested. PMID: 25577493
  3. human sperm possess an aldo-keto reductase on their membrane surface and are thus enzymatically protected against reactive aldehyde species both in the male and female reproductive tract PMID: 22970857
  4. Strong candidate gene for mitochondrial disease, based on recessive mutations detected in infantile patients PMID: 22277967
  5. AKR1B15 and Akr1b16 genes are expressed as functional proteins in human and murine tissues PMID: 21276782
  6. Observational study of gene-disease association. (HuGE Navigator) PMID: 20877624

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Subcellular Location
[Isoform 1]: Mitochondrion.; [Isoform 2]: Cytoplasm, cytosol.
Protein Families
Aldo/keto reductase family
Tissue Specificity
Widely expressed. Expressed at highest levels in steroid-sensitive tissues, such as placenta, testis and adipose tissue.
Database Links

HGNC: 37281

OMIM: 616336

KEGG: hsa:441282

STRING: 9606.ENSP00000389289

UniGene: Hs.729418

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