Recombinant Human Putative teratocarcinoma-derived growth factor 3 (TDGF1P3)

Code CSB-BP344744HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-EP344744HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TDGF1P3
Uniprot No.
Alternative Names
TDGF1P3; CRIPTO3; TDGF2; TDGF3; Putative teratocarcinoma-derived growth factor 3; Cripto-3 growth factor; Epidermal growth factor-like cripto protein CR3; Teratocarcinoma-derived growth factor 1 pseudogene 3
Species
Homo sapiens (Human)
Expression Region
31-188
Target Protein Sequence
LGHQEFARPSRGDLAFRDDSIWPQEEPAIRPRSSQRVLPMGIQHSKELNRTCCLNGGTCMLESFCACPPSFYGRNCEHDVRKENCGSVPHDTWLPKKCSLCKCWHGQLRCFPQAFLPGCDGLVMDEHLVASRTPELPPSARTTTFMLAGICLSIQSYY
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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 Q&A
Q:

Regarding your protein CSB-BP344744HU, I'm interested in 1mg of the Baculovirus-expressed protein, and was wondering if it was possible to have the C-terminus tagged with 6xHis-tag, and NO tag on the N-terminus. Would these be suitable specifications?
Thanks!

A:
Thanks for your inquiry.
As you know, this is a semi-customized protein that we haven't expressed before, we can try the Baculovirus-expressed protein with 6xHis-tag at the C-terminus, but we can't 100% guarantee. The success rate of expressing the protein with C-terminal 6xHis-tagged is 95.25%.
Please confirm if you can accept other similar tag if the C-terminal 6xHis-tagged is failed. In addition, please remark your requirement for the tag when placing the order.

Target Background

Function
Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm. Activates the Nodal-dependent signaling pathway.
Subcellular Location
Cell membrane.
Protein Families
EGF-CFC (Cripto-1/FRL1/Cryptic) family
Tissue Specificity
Expressed weakly in lung, colon and breast. Expressed also strongly in primary cancer tissues; lung and colon cancers.
Database Links

HGNC: 11703

OMIM: 187395

UniGene: Hs.592361

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