Product Details

Purity

>85% (SDS-PAGE)

Target Names

RBP1

Uniprot No.

P09455

Alternative Names

Cellular retinol binding protein; Cellular retinol-binding protein; Cellular retinol-binding protein I; CRABP I; CRBP; CRBP-I; CRBP1; CRBPI; RBP 1; RBP1; RBPC; RET1_HUMAN; Retinol binding protein 1 cellular; Retinol-binding protein 1

Species

Homo sapiens (Human)

Expression Region

1-135

Target Protein Sequence

MPVDFTGYWK MLVNENFEEY LRALDVNVAL RKIANLLKPD KEIVQDGDHM IIRTLSTFRN YIMDFQVGKE FEEDLTGIDD RKCMTTVSWD GDKLQCVQKG EKEGRGWTQW IEGDELHLEM RVEGVVCKQV FKKVQ

Protein Length

Full Length

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

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Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

■ Q&A

We are interested in retinol binding protein (human) full-length. Without tags, without sodium azide and we would like it lyophilized. Can you provide this request?

Thanks for your inquiry.
1.Regarding the protein without sodium azide, there is no problem, all of our proteins don't contain any sodium azide.
2.But as for the no tag protein, we can have a try but we can't guarantee.
Please remark your requirement for tag removal if you need the untagged protein or tag removal when placing the order. We can try enzyme digestion, but we can't guarantee 100% successfully.The overall success rate of enzyme digestion data analysis is 75%-86%.
Not all protein tags can be removed as some proteins will be very unstable after tag removal.
If we succeed in removing the tag, we will charge for extra cost.
If we fail in removing the tag, we won’t charge for tag removal fees and provide the fusion protein, and remark this information in datasheet as follows
“Note: The laboratory determined that the Tag on your protein could not be removed with standard laboratory procedures. Your protein is being supplied with the Tag intact.”
Generally, the delivery time will be extended for 3 days.
3.We can provide lyophilization service for free, lead time will be extended for 3 working days, please be noted that we will add 6% trehalose added as the protective agent during the lyophilization. Please check if this is OK for you.

Target Background

Function

Cytoplasmic retinol-binding protein. Accepts retinol from the transport protein STRA6, and thereby contributes to retinol uptake, storage and retinoid homeostasis.

Gene References into Functions

Low CRBP1 expression is associated with low radiation sensitivity in rectal cancer. PMID: 28291773
This conformational flexibility of human apo-CRBP1 facilitates interaction with the ligands, whereas the more rigid holoprotein structure protects the labile retinoid moiety during vitamin A transport. PMID: 26900151
Hypermethylation of CRBP1 gene is associated with Epstein-Barr virus-associated gastric carcinomas. PMID: 25720522
Further studies are needed to explore new therapeutic strategies aimed at restoring CRBP-1-mediated intracellular retinol trafficking and the meaning of CRBP-1 expression in cancer patients screening for a more personalized and efficacy retinoid therapy PMID: 25879031
RBP1 expression was reduced in tissue and endometrial stromal cells derived from endometritis lesions versus eutopic endometruim in humans and mice. PMID: 25143356
Functional hepatic stellate cells coexpressing both LRAT and CRBP-1, that continue to maintain the ability to store vitamin A, contribute in part to the development of portal and parenchymal fibrogenesis in patients with viral hepatitis. PMID: 23890161
Factors involved in the retinoid pathway, in particular upregulation of CRBP, CRABP1 and CRABP2, also showed differential expression in tumors with different histological subtypes PMID: 24269351
CRBP-1 loss in G2/G3 ovarian carcinomas and increased apoptotic susceptibility to retinoids. PMID: 24982334
Suggest CRBP1 gene as a tumor suppressor gene for cervical cancer. PMID: 24040446
High urine retinol binding protein is associated with interstitial fibrosis and tubular atrophy plus inflammation. PMID: 23414180
RBP1 promoter hypermethylation is found in nearly all IDH1 and IDH2 mutant gliomas Because RBP1 is involved in retinoic acid synthesis, results suggest that dysregltion of RA metabolism may contribute to glioma formation along the IDH1/IDH2-mutant pathway PMID: 22945948
CRBP-1 plays a role in ventricular remodeling after myocardial infarction allegedly through its RA binding activity. PMID: 22449228
Increased expression and unmethylated promoter of the CRBP1 gene could be considered as markers for larynx cancer. PMID: 22049223
Data show that CRBPI and CRBPII bind 9-cis-retinol and 9-cis-retinal with high affinities, albeit with affinities somewhat lower than for all-trans-retinol and all-trans-retinal. PMID: 21382444
STRA6 not only is a vitamin A transporter but also is a cell-surface signaling receptor activated by the RBP-retinol complex. PMID: 21368206
CRBP1 gene could have a tumor suppressive function in bladder cancer. PMID: 21042705
It is likely that mutations in RGR, RBP3, and possibly RBP1 occur rarely in inherited retinal dystrophies. PMID: 21067480
protein-ligand interaction differences between CRBP-I and CRBP-II PMID: 19965581
a high CRBP-1 expression is associated with smooth muscle malignancy and suggest that CRBP-1 expression represents a new useful marker for the classification of unusual variants of smooth muscle tumors PMID: 12111198
CRBP1 has a role in vitamin A metabolism and its absence may play a role in ovarian cancer oncogenesis PMID: 12631600
may serve as a surrogate marker for graft dysfunction early after kidney transplantation PMID: 12826154
in liver cancer modulation of CRBP-1 expression in HCCs contributes to tumor growth and progression via retinoid-mediated signaling and disruption of cellular vitamin A homeostasis. PMID: 12883492
Colocalization of plasma retinol-binding protein with RPE65 and affinity binding suggest a direct interaction of RPE65 with plasma retinol-binding protein in cultured human keratinocytes that might be involved in retinoid uptake of keratinocytes. PMID: 15009723
in fibrotic or cirrhotic liver, the different patterns of expression for CRBP-1 and alpha-SMA allow the distinction of different subsets of fibroblastic cells involved in fibrogenesis and septa formation PMID: 15094224
CRBP-I downregulation in cancer promotes tumor progression through inhibition of retinoic acid receptor activity and derepression of PI3K/Akt signaling via a novel mechanism. PMID: 15608670
The widely expressed CRBP1, which binds all-trans-retinol with much higher affinity than all-trans-retinaldehyde, restricts the oxidation of all-trans-retinol by retinol dehydrogenase 12 but has little effect on the reduction of all-trans-retinaldehyde. PMID: 15865448
CRBP-1+ cells increased in squamous intraepithelial lesions and slightly decreased in invasive squamous cell carcinomas. PMID: 17593084
Retinoic acid and retinol regulate expression of cellular retinol binding protein 1 and cellular RA binding protein 2 in fibroid and myometrial cells. PMID: 18343808
CRBP-1 clearly stains liver lobular HSCs without reacting with smooth muscle cells (SMCs) and shows variable cholangiocyte p PMID: 19052772
Methylation of CLDN6, FBN2, RBP1, RBP4, TFPI2, and TMEFF2 in esophageal squamous cell carcinoma. PMID: 19288010

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Subcellular Location

Cytoplasm. Lipid droplet.

Protein Families

Calycin superfamily, Fatty-acid binding protein (FABP) family

Tissue Specificity

Detected in nearly all the tissues with higher expression in adult ovary, pancreas, pituitary gland and adrenal gland, and fetal liver.

Database Links

HGNC: 9919

OMIM: 180260

KEGG: hsa:5947

STRING: 9606.ENSP00000232219

UniGene: Hs.529571

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Recombinant Human Retinol-binding protein 1 (RBP1)

Product Details

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Target Background

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