Recombinant Human AF4/FMR2 family member 3 (AFF3), partial

Code CSB-YP001414HU
MSDS
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Source Yeast
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Code CSB-EP001414HU
MSDS
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Source E.coli
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Code CSB-EP001414HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP001414HU
MSDS
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Source Baculovirus
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Code CSB-MP001414HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
AFF3
Uniprot No.
Alternative Names
AF4/FMR2 family member 3; AFF3; AFF3_HUMAN; LAF4; Lymphoid nuclear protein 4; Lymphoid nuclear protein related to AF4; MLLT2 like; MLLT2 related protein; Protein LAF-4
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Putative transcription activator that may function in lymphoid development and oncogenesis. Binds, in vitro, to double-stranded DNA.
Gene References into Functions
  1. Data identified AFF3 as an interactive protein with beclin1 and suggest that AFF3 is probably a key regulator of Beclin-1. PMID: 28401970
  2. Both the AFF3 and NTM triglyceride associations were replicated among Multi-ethnic Study of Atherosclerosis study participants (P = 1.00 x 10(-7) and 8.00 x 10(-5), respectively). PMID: 25819087
  3. FRA2A-expressing individuals have mosaic expansions of the AFF3 CGG repeat. PMID: 24763282
  4. Significant evidence for association of AFF3 rs10865035 with systgemic lupus erythmatgosus was detected. PMID: 22983539
  5. overexpression of AFF2/3/4 interferes with the organization and/or biogenesis of nuclear speckles. PMID: 21330300
  6. The LAF4 gene mapped on chromosome region 2q11.2-q12 has been identified as a fusion partner of the MLL gene in a case of infant acute lymphoblastic leukemia. PMID: 12203795
  7. This gene is fused to MLL in childhood acute lymphoblastic leukemia with t(2;11)(q11;q23). PMID: 12743608
  8. LAF-4 may be a proto-oncogene that is transcriptionally activated in some cases of breast cancer. PMID: 15704140
  9. array comparative genomic hybridization (CGH) analysis revealed a de novo interstitial microdeletion of 500 kb on chromosome 2q11.1 containing the LAF4/AFF3 (lymphoid-nuclear-protein-related AF4) gene PMID: 18616733
  10. A novel fusion 5'AFF3/3'BCL2 originated from a t(2;18)(q11.2;q21.33) translocation in follicular lymphoma. PMID: 18622426
  11. AFF3 is a novel rheumatoid arthritis susceptibile gene. PMID: 19359276

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Subcellular Location
Nucleus.
Protein Families
AF4 family
Tissue Specificity
Preferentially expressed in lymphoid tissues, highest levels being found in the thymus.
Database Links

HGNC: 6473

OMIM: 601464

KEGG: hsa:3899

STRING: 9606.ENSP00000348793

UniGene: Hs.444414

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