Recombinant Human Absent in melanoma 1 protein (AIM1), partial

Code CSB-YP896518HU
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Source Yeast
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Code CSB-EP896518HU
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Source E.coli
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Code CSB-EP896518HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP896518HU
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Source Baculovirus
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Code CSB-MP896518HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CRYBG1
Uniprot No.
Alternative Names
Absent in melanoma 1; Absent in melanoma 1 protein; AIM1; AIM1_HUMAN; Beta/gamma crystallin domain-containing protein 1; CRYBG1; ST4
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May function as suppressor of malignant melanoma. It may exert its effects through interactions with the cytoskeleton.
Gene References into Functions
  1. Single-molecule Force Spectroscopy Reveals the Calcium Dependence of the Alternative Conformations in the Native State of a betagamma-Crystallin Protein. PMID: 27378818
  2. Both DNMT1 and AIM1 show maternal allele-specific methylation and paternal allele-specific transcription. PMID: 24094292
  3. AIM1, ERGIC1, and TPX2 were shown to be highly expressed especially in prostate cancer tissues, and high mRNA expression of ERGIC1 and TMED3 associated with AR and ERG oncogene expression PMID: 22761906
  4. Methylation status of AIM1 in the prostate cancer specimen may predict for time to recurrence in Gleason 3 + 4 = 7 patients undergoing prostatectomy. PMID: 22127895
  5. AIM1 promoter hypermethylation was found in higher frequency (P = 0.005) in metastatic melanoma (65%) than in primary melanomas (38%). PMID: 22402438
  6. the structure of AIM1g1, a single betagamma-crystallin domain from the protein absent in melanoma 1, was determined PMID: 16511323
  7. Absent in melanoma 1 (AIM1), an unusual member of the superfamily , contains 12 betagamma-crystallin motifs in six domains. Some of these motifs diverge considerably from the canonical motif sequence. AIM1g1, the first betagamma-crystallin domain of AIM1. PMID: 18582473
  8. tumor-promoting effect of deletion in 6q21, that included genes PRDM1, ATG5 and AIM1 PMID: 19194464

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Protein Families
Beta/gamma-crystallin family
Database Links

HGNC: 356

OMIM: 601797

KEGG: hsa:202

STRING: 9606.ENSP00000358062

UniGene: Hs.724262

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