Recombinant Human Bcl10-interacting CARD protein (C9orf89)

Code CSB-YP842711HU
MSDS
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Source Yeast
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Code CSB-EP842711HU
MSDS
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Source E.coli
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Code CSB-EP842711HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP842711HU
MSDS
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Source Baculovirus
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Code CSB-MP842711HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CARD19
Uniprot No.
Alternative Names
Bcl10 interacting protein with CARD protein; Bcl10-interacting CARD protein; BINCA_HUMAN; BinCARD; C9orf89; Chromosome 9 open reading frame 89
Species
Homo sapiens (Human)
Expression Region
1-228
Target Protein Sequence
MTDQTYCDRL VQDTPFLTGH GRLSEQQVDR IILQLNRYYP QILTNKEAEK FRNPKASLRV RLCDLLSHLQ RSGERDCQEF YRALYIHAQP LHSRLPSRHA LRKFHITNHA CLVLARGGHP SLPLMAWMSS MTTQVCCSPG LASPLASAPP QRPPSGPEGR VWQAQAVQML VSVSHFLPLP PSLSHGSFHT AWGILYVHSC PSFSNLIPRG SLHVCVDSNL VPTAAWRS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Plays a role in inhibiting the effects of BCL10-induced activation of NF-kappa-B. May inhibit the phosphorylation of BCL10 in a CARD-dependent manner.
Gene References into Functions
  1. the crystal structure of BinCARD reveals that all three cysteines were oxidized PMID: 23633586
  2. BinCard inhibits BCL10-mediated activation of NF-kappa B. PMID: 15637807
Subcellular Location
[Isoform 1]: Nucleus. Note=Coexpression with BCL10 induced translocation from nucleus to cytosol.; [Isoform 2]: Endoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein.
Tissue Specificity
Expressed in ovary, testis, placenta, skeletal muscle, kidney, lung, heart and liver (at protein level). Expressed in thymus and brain.
Database Links

HGNC: 28148

OMIM: 617726

KEGG: hsa:84270

UniGene: Hs.434213

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