Recombinant Human C-type lectin domain family 10 member A (CLEC10A), partial

Code CSB-YP811601HU
MSDS
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Source Yeast
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Code CSB-EP811601HU
MSDS
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Source E.coli
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Code CSB-EP811601HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP811601HU
MSDS
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Source Baculovirus
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Code CSB-MP811601HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CLEC10A
Uniprot No.
Alternative Names
C type (calcium dependent carbohydrate recognition domain) lectin superfamily member 13 (macrophage derived); C type (calcium dependent carbohydrate recognition domain) lectin superfamily member 14 (macrophage derived); C type lectin domain family 10; member A; C type lectin superfamily member 14; C-type lectin domain family 10 member A; C-type lectin superfamily member 14; CD301; CD301 antigen; CD301A; CLC10_HUMAN; CLEC10A; CLECSF13; CLECSF14; HML; HML2; Lectin; Macrophage C type lectin; MACROPHAGE GALACTOSE-TYPE C-TYPE; Macrophage lectin 2 (calcium dependent); Macrophage lectin 2; MGL; Mgl1
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Probable role in regulating adaptive and innate immune responses. Binds in a calcium-dependent manner to terminal galactose and N-acetylgalactosamine units, linked to serine or threonine. These sugar moieties are known as Tn-Ag and are expressed in a variety of carcinoma cells.
Gene References into Functions
  1. this paper shows that key gene of intermediate proinflammatory monocytes, such as CLEC10A, is expressed three- to fourfold more in juvenile idiopathic arthritis-enthesitis-related arthritis PMID: 27706807
  2. BRAF(V600E) mutation induces MGL ligand expression, thereby providing a direct link between oncogenic transformation and aberrant expression of immunosuppressive glycans in colorectal neoplasms. PMID: 26172302
  3. These results indicate a role of MGL as an immunomodulator within the tumour microenvironment interfering with Treg functions, suggesting its possible use in the design of anticancer vaccines. PMID: 26147970
  4. expression of GalNAc moieties mirrors the T cell activation status, and thus only highly stimulated T cells are prone to the suppressive action of MGL. PMID: 23918927
  5. MGL triggering synergized with TLR2-induced pathways, leading to elevated IL-10 mRNA levels & enhanced TNF-alpha mRNA stability. It promoted phosphorylation of the ERK & CREB, fine-tuning the DC maturation phenotype. PMID: 23744646
  6. Described is the use of recombinant CLEC10A (CD301), a human glycoreceptor of the C-type lectin family, for the detection of ligands in sections from formalin-fixed, paraffin-embedded normal and cancerous mammary tissues. PMID: 23275449
  7. MGL engagement improved DC performance as antigen-presenting cells, promoting the upregulation of maturation markers, a decrease in phagocytosis, an enhancement of motility, and most importantly an increase in antigen-specific CD8(+) T-cell activation. PMID: 22531918

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Subcellular Location
Membrane; Single-pass type II membrane protein.
Database Links

HGNC: 16916

OMIM: 605999

KEGG: hsa:10462

STRING: 9606.ENSP00000254868

UniGene: Hs.54403

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