Recombinant Human Calpain small subunit 1 (CAPNS1)

Code CSB-YP004503HU
MSDS
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Source Yeast
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Code CSB-EP004503HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP004503HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP004503HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CAPNS1
Uniprot No.
Alternative Names
30K; Calcium activated neutral proteinase; Calcium activated neutral proteinase small subunit; Calcium dependent protease small subunit 1; Calcium dependent protease small subunit; Calcium-activated neutral proteinase small subunit; Calcium-dependent protease small subunit 1; Calcium-dependent protease small subunit; Calpain 4; Calpain 4 small subunit (30K); Calpain 4 small subunit; Calpain regulatory subunit; Calpain S1; Calpain small polypeptide; Calpain small subunit 1; Calpain4; CalpainS1; CANP; CANP small subunit; CANPS; CAPN 4; CAPN S1; CAPN4; CAPNS 1; CAPNS; Capns1; CDPS; CPNS1_HUMAN; CSS 1; CSS1
Species
Homo sapiens (Human)
Expression Region
1-268
Target Protein Sequence
MFLVNSFLKG GGGGGGGGGG LGGGLGNVLG GLISGAGGGG GGGGGGGGGG GGGGGGTAMR ILGGVISAIS EAAAQYNPEP PPPRTHYSNI EANESEEVRQ FRRLFAQLAG DDMEVSATEL MNILNKVVTR HPDLKTDGFG IDTCRSMVAV MDSDTTGKLG FEEFKYLWNN IKRWQAIYKQ FDTDRSGTIC SSELPGAFEA AGFHLNEHLY NMIIRRYSDE SGNMDFDNFI SCLVRLDAMF RAFKSLDKDG TGQIQVNIQE WLQLTMYS
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
Gene References into Functions
  1. that Capn4 promotes cell proliferation by increasing MAPK7 expression PMID: 29331389
  2. Calpain small subunit 1 (Capn4) overexpression increased the protein level of cleaved talin and and activated the focal adhesion kinase (FAK)/AKT/MAPK signaling in 786-O cells, while Capn4 silencing decreased the protein level of cleaved talin in Caki-1 cells. PMID: 29648579
  3. Capn4 and miR-1271 may serve as potential therapeutic targets for the treatment of colorectal cancer. PMID: 29331762
  4. MALAT1 and Capn4 were up-regulated while miR-124 expression was down-regulated in nasopharyngeal carcinoma cell lines. PMID: 28857668
  5. Capn4 promotes epithelial-mesenchymal transition in human melanoma cells through activation of the Wnt/beta-catenin pathway. PMID: 27878263
  6. CAPNS1 depletion is coupled to impairment of breast cancer cell growth. PMID: 26771715
  7. High levels of Capn4 expression were associated with lymph node metastasis and large tumor size in non-small cell lung cancer patients.Capn4 promotes non-small cell lung cancer progression via upregulation of matrix metalloproteinase 2. PMID: 25636510
  8. HBXIP was able to up-regulate Capn4 at the levels of promoter. PMID: 25304384
  9. The overexpression of Capn4 is a novel negative prognostic marker, and Capn4 may be used as a new target in therapeutic strategies for human glioma. PMID: 24628706
  10. Capn4 can contribute to hepatocellular carcinoma growth and metastasis PMID: 24962955
  11. Capn4 mRNA and protein are increased in human clear cell renal cell carcinoma tissues and higher Capn4 levels are associated with advanced clinical tumour stage and shorter overall survival. PMID: 24514433
  12. Capn4 upregulated MMP2 via nuclear factor-kappaB activation, manifested by increased phosphorylation of p65, a subunit of NF-kappaB. PMID: 24703594
  13. This study suggests that augmented calpain activity is associated with increased incidence of thrombosis under hypoxic environments. PMID: 24297866
  14. Capn4 overexpression was implicated in ICC metastasis/invasion, and Capn4 overexpression may be used as a molecular therapeutic target for intrahepatic cholangiocarcinoma PMID: 23349941
  15. The authors conclude that hepatitis B virus X protein upregulate Capn4 through NF-kappaB/p65 to promote migration of hepatoma cells. PMID: 20419804
  16. CAPNS1 depletion is coupled to reduced levels of H2AX phosphorylation, not only in Ras(v12) induced BJ-ET fibroblasts, but also in a number of cellular systems upon genotoxic stress PMID: 20107320
  17. Genetic variations in LEP, CAPNS1 and ALOX15 show significant association with IL-6 expression. PMID: 19942621
  18. calpain-mediated XIAP degradation contributes to initiation of apoptosis in normal neutrophils and dysfunction of this regulatory pathway can lead to pathological neutrophil accumulation. PMID: 12121983
  19. Whereas alphaPIX guanine nucleotide exchange factor activity contributes to enhanced formation of cellular protrusions, the GEF-independent association with calpain 4 leads to induction of a yet unknown signaling cascade resulting in cell spreading. PMID: 15611136
  20. results reveal that especially NRF-1, along with AP-1 and, to a minor extent, an Sp1 site, is essential for human CAPNS1 promoter activity and gene expression PMID: 18234454
  21. c-Myc regulates calpain activity through calpastatin; apoptosis induced by calpain inhibition is dependent on c-Myc, and calpastatin knockdown promotes transformation in c-Myc-negative cells PMID: 18544539
  22. RasGAP and Capns1 interaction in oncogenic Ras cells is involved in regulating migration and cell survival. PMID: 18761085

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Subcellular Location
Cytoplasm. Cell membrane.
Database Links

HGNC: 1481

OMIM: 114170

KEGG: hsa:826

STRING: 9606.ENSP00000246533

UniGene: Hs.515371

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