Recombinant Human Cell surface glycoprotein MUC18(MCAM),partial

Code CSB-YP013563HU
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Source Yeast
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Code CSB-EP013563HU
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Source E.coli
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Code CSB-EP013563HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP013563HU
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Source Baculovirus
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Code CSB-MP013563HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names MCAM
Uniprot No. P43121
Alternative Names A32 antigen; CD 146; CD146; CD146 antigen; Cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; Gicerin; Mcam; Melanoma adhesion molecule; Melanoma associated antigen A32; Melanoma associated antigen MUC18; Melanoma associated glycoprotein MUC18; Melanoma cell adhesion molecule; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; MelCAM; MUC 18; MUC18; MUC18_HUMAN; S endo 1; S endo 1 endothelial associated antigen; S-endo 1 endothelial-associated antigen
Species Homo sapiens (Human)
Protein Length Extracellular domain
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
Gene References into Functions
  1. MCAM coordination of apical-basal polarity and planar cell polarity provides insight into the general mechanisms of morphogenesis. PMID: 28589943
  2. CD146 suppresses BC progression as a target of CD44-downstream signaling PMID: 29121955
  3. Cultured early passage ASCs contained low levels of CD146 mRNA, which was expressed in two different splicing variants, at a relatively high amount of the CD146-long form and at a relatively low amount of the CD146-short form. ASCs contained low levels of CD146 protein, which consisted predominantly long form and a small amount of short form. PMID: 28549249
  4. In summary, we have shown that MUC18/Muc18 may serve as a regulator of airway inflammation and mucus overproduction, two important features of type 2-high asthma. Our data suggest that MUC18/Muc18 or its downstream signaling mediators may be targets for potential therapeutic agents. PMID: 28451734
  5. CD146 promoter polymorphisms were not associated with the risk of clear cell renal carcinoma in Chinese population. The rs3923594 was an independent predictor of recurrence in with localized ccRCC. PMID: 28626293
  6. We demonstrate that ER(+) breast cancers contain two CAF subtypes defined by CD146 expression. CD146(neg) CAFs suppress ER expression in ER(+) breast cancer cells, decrease tumor cell sensitivity to estrogen, and increase tumor cell resistance to tamoxifen therapy PMID: 27702820
  7. CD146 functions as a suppressor of tumorigenesis and cancer stemness in CRC through inactivating the canonical Wnt/beta-catenin cascade PMID: 27302922
  8. data identify the anatomy and phenotype of a novel class of committed myogenic progenitor in human post-natal skeletal muscle of subendothelial cells associated with the abluminal surface of microvascular compartment distinct from satellite cells PMID: 29186180
  9. These findings identify CD146 as a novel retention signal that traps macrophages within the artery wall, and a promising therapeutic target in atherosclerosis treatment. PMID: 28084332
  10. CD146 was expressed in all cases of Ph-positive B- cell acute lymphoblastic leukemia and in the vast majority of T-cell acute lymphoblastic leukemia PMID: 26102234
  11. Our results suggest that MCAM may serve as a novel therapeutic target to overcome chemoresistance in SCLC. PMID: 28646020
  12. Authors show that KDM3A regulates MCAM expression both through a direct mechanism, involving modulation of H3K9 methylation at the MCAM promoter, and an indirect mechanism, via the Ets1 transcription factor. PMID: 28319067
  13. increment of CD146 expression indicates gradual change of cultured annulus fibrosus cells to express a contractile phenotype and that transforming growth factor beta1 enhances this cellular commitment PMID: 27273299
  14. CD146 positivity in immunohistological analysis of 11 MRT patient samples was associated with poor patient outcomes. These results suggest that CD146 defines a distinct sub-population in MRT with high tumorigenic capacity and that this marker represents a promising therapeutic target. PMID: 27041577
  15. Promoter methylation of MCAM, ERalpha and ERbeta have a potential to be utilized as biomarker for the early detection of prostate cancer (PC) as their sensitivity and specificity seem to be better than serum PSA. PMID: 28147335
  16. MCAM promotes tamoxifen resistance by transcriptionally suppressing ERalpha expression and activating the AKT pathway, followed by induction of epithelial-mesenchymal transition. PMID: 27838413
  17. High MCAM expression is associated with lung metastasis in malignant melanoma. PMID: 27151304
  18. Results provide evidence that MUC18 promotes viral infections both in vivo and in vitro. PMID: 27701461
  19. soluble CD146 is released from the peripheral vasculature in response to venous stretch and may reflect systemic congestion in chronic heart failure patients PMID: 28062630
  20. results indicate that CD146 can be targeted in vivo by the radiolabeled OI-3 antibodies PMID: 27776176
  21. Findings suggest that decreased CD146 expression in cancer-associated fibroblasts promotes pancreatic cancer progression. PMID: 26373617
  22. METCAM/MUC18 positively promotes tumorigenesis of human breast cancer SK-BR-3 cells via increasing the signal in survival and proliferation pathways. PMID: 27125403
  23. sCD146 levels are elevated in patients with systemic sclerosis, but decreased sCD146 levels are observed in SSc patients with pulmonary arterial hypertension PMID: 27726047
  24. Nestin and CD146 are expressed in breast cancer cells with highly aggressive potency. They might contribute to disease relapse in breast cancer by activating the epithelial-mesenchymal transition pathway and assist tumor neovascularization. PMID: 28347241
  25. The results demonstrated isolation of specific scFv with frequency of 40 % which showed significant binding with the epitope in both ELISA and fluorescence-activated cell sorting (FACS) analyses. The antibody inhibited the migration (76 %) and invasion (67 %) of MUC18 positive cell line. The results suggest the specific anti-MUC18 scFv as an effective antibody for breast cancer immunotherapy PMID: 27565656
  26. We provide the first report that pro-angiogenic genes PECAM1, PTGS1, FGD5, and MCAM may play a vital role in pathological dermal angiogenesis disorders of psoriasis. PMID: 26748901
  27. Results showed that increased human METCAM/MUC18 expression in ovarian cancer SK-OV-3 cells suppressed tumorigenesis and ascites formation in nude mice, suggesting that human METCAM/MUC18 plays a suppressor role in the progression of ovarian cancer, perhaps by reducing proliferation and angiogenesis. PMID: 26906545
  28. CD146 dexpression efines a subpopulation of human mesenchymal stem cells capable of bone formation and in vivo trans-endothelial migration. PMID: 26753846
  29. Results showed that CD146 promoted metastasis of hepatocellular carcinoma (HCC) cells and predicted poor prognosis of HCC patients. CD146 induced epithelial mesenchymal transition through probably IL-8 up-regulation and STAT1 down-regulation. PMID: 26928402
  30. Data indicate that CD146 antigen is an effective cell surface marker for enriching tumor-propagating cells (TPCs) in primary sarcomas. PMID: 26517673
  31. MUC18 is an independent prognostic factor for clear cell renal cell carcinoma PMID: 26617818
  32. CD146 is a novel and useful marker for predicting senescence in human umbilical cord blood-derived Mesenchymal stem cells (hUCB-MSCs), and CD146 can potentially be applied in quality-control assessments of hUCB-MSC-based therapy. PMID: 26941359
  33. ZBTB7A directly binds to the promoter and transcriptionally represses the expression of MCAM, establishing ZBTB7A as a bona fide transcriptional repressor of MCAM PMID: 25995384
  34. The expression of CD146 and HIF1a was positively correlated with EGFR and CD31, respectively in salivary gland adenoid cystic carcinoma. PMID: 25997612
  35. a model that CD166 regulates MCAM through a signaling flow from activation of PI3K/AKT and c-Raf/MEK/ERK signaling to the inhibition of potential MCAM ubiquitin E3 ligases, betaTrCP and Smurf1 PMID: 26004137
  36. Combined EpCAM/MCAM CellSearch enrichment thus increased the CTC detection rate. PMID: 25552367
  37. Results identified MCAM as a novel YAP target in hepatocellular carcinoma (HCC) but not in breast and colon cancer cells. MCAM serum levels were specifically elevated in HCC suggesting it as a specific diagnostic tool for HCC. PMID: 25728681
  38. CD146, P53, and Ki-67 are overexpressed in uterine sarcoma PMID: 26293576
  39. These data suggest CDCP1 expression can be used to identify a subset of marrow fibroblasts functionally distinct from CD146+ fibroblasts. PMID: 25275584
  40. Results show that that CD146 is expressed in 41% of gastric neoplasm cells and correlated positively with lymph node metastasis and epithelial-mesenchymal transition markers making it a good prognostic factor. PMID: 22754372
  41. MCAM is a major GAL-1 ligand is largely dependent on melanoma malignancy. PMID: 25756799
  42. MCAM is expressed by effector CD8+ T lymphocytes and it is strikingly upregulated during multiple sclerosis relapses. MCAM blockade restricts the transmigration of CD8(+) T lymphocytes across human blood-brain barrier endothelial cells. PMID: 25869475
  43. HuMETCAM/MUC18 levels in ovarian carcinomas and metastatic lesions were significantly higher than in normal tissues and cystadenomas. PMID: 25510693
  44. In peripheral stenotic arteriosclerotic disease the proangiogenic potency of MUC18 may play a role in angiogenesis of collaterals, whereas in dilatative aortic diseases the induction of collaterals is typically not evident. PMID: 25729916
  45. Suggest endothelial CD146 as a target for specific drug delivery in hepatocellular carcinoma. PMID: 25238265
  46. Data proposed a novel signaling mechanism by which Sema 3A regulates PTEN, FOXO 3a and MelCAM in a coordinated manner that leads to suppression of breast cancer growth and angiogenesis. PMID: 24727891
  47. Authors conclude therefore that ETs upregulate MCAM in an Akt and ERK/MEK-dependent, but CREB-independent manner, providing an understanding for possible pharmacologic intervention in progressing melanoma. PMID: 24743054
  48. expression behaves as a molecular warning of melanoma progression PMID: 24902661
  49. With the ability of migration and survival in the advanced osteoarthritis cartilage, CD146+ chondroprogenitors might be "tissue-specific" for cartilage tissue regeneration. PMID: 25266708
  50. functional characterization of N-acetylglucosaminyltransferases III and V in human melanoma cells PMID: 24726881
  51. CD146 and its soluble form correlate with the development of inflammation of atherosclerosis and plaque instability; CD146 may be a promising biomarker for monitoring the development and instability of atherosclerotic plaque in carotid disease patients PMID: 24612514
  52. MCAM expression in surgically treated non-small cell lung cancer is clearly associated with lymph node metastasis and poor prognosis. PMID: 23749325
  53. Mean sCD146 levels were significantly higher in type 1 diabetes patients than in controls. ROC curves for sCD146 significantly differed in differentiating type 1 diabetics from healthy controls. PMID: 24139427
  54. CD146+ human umbilical cord perivascular cells maintain stemness under hypoxia and as a cell source for skeletal regeneration. PMID: 24204598
  55. Wingless-type protein Wnt5a uses CD146 as a receptor to regulate cell migration. PMID: 24335906
  56. A strong expression of CD146 molecule is associated with a commitment to a vascular smooth muscle cell lineage in mesenchymal stem cells. PMID: 24188055
  57. The CEC count and the plasma level of sCD146 were significantly higher in NSCLC patients. PMID: 24473396
  58. CD146 and Lxn increased tumor migration and invasion of thyroid cancer. PMID: 23712706
  59. miR-573 upregulation inhibited melanoma cell proliferation and invasion, and overexpression of melanoma cell adhesion molecule (MCAM) could alleviate the effect of miR-573 on melanoma cells. PMID: 23670160
  60. Data indicate that the expression of the melanoma cell adhesion molecule (MCAM) in mesenchymal stromal cells determines their fate and regulates the maintenance of hematopoietic stem and progenitor cells through direct cell-cell contact. PMID: 22801967
  61. CD146 expression is characterized in circulating T cells from healthy controls and patients with stable, well-controlled connective tissue diseases; both CD4 and CD8 T cells are capable of up-regulating CD146 expression in response to stimulation. PMID: 23738744
  62. Identified a novel negative regulator of angiogenesis, microRNA 329 (miR-329), that directly targeted CD146 and inhibited CD146-mediated angiogenesis in vitro and in vivo. PMID: 23878390
  63. Alterations in CD146 are involved in the basic etiology of COPD/pulmonary emphysema. PMID: 23649916
  64. propose that in human, sCD146 could represent both an attractive biomarker of placental vascular development and a therapeutic target in pregnancy complications associated with pathological angiogenesis PMID: 23108590
  65. cerebrospinal fluid levels of sCD146 may provide a potential marker for monitoring disease activity in multiple sclerosis patients PMID: 23333866
  66. MUC18 exerts a proinflammatory function during lung bacterial infection. PMID: 23256918
  67. Data show that CD146+ perivascular cells are a subset of mesenchymal stem/stromal cells (MSCs), able to support hematopoietic stem and progenitor cells (HSPCs) and regulate lineage commitment in vitro through cell-to-cell interaction. PMID: 23412095
  68. Soluble melanoma cell adhesion molecule (sMCAM/sCD146) promotes angiogenic effects on endothelial progenitor cells through angiomotin PMID: 23389031
  69. CD146 gene expression can be useful for predicting early recurrence and stratifying the patients into risk groups for possible adjuvant treatment PMID: 22826148
  70. REVIEW: newly identified functions and the underlying mechanisms of CD146 PMID: 23266426
  71. CD146 and its novel ligand Galectin-1 have a role in apoptotic regulation of endothelial cells PMID: 23223580
  72. CD146 expression is associated with an EMT phenotype PMID: 22952755
  73. MCAM serves as a potential biomarker for multiple sclerosis and represents a valuable target for the treatment of neuroinflammatory conditions. PMID: 22975388
  74. MCAM expression in ovarian cancer is highly correlated with the metastatic potential of the cancer. PMID: 22610942
  75. sCD146 levels are significantly higher in women presenting a history of recurrent fetal losses. PMID: 22635231
  76. The preferential interaction between soluble CD146 and CD16 positive monocytes may contribute to activation and homing of monocytes into inflamed vascular beds. PMID: 22311021
  77. Expression of CD146 and P-selectin in patients with type 2 diabetes is elevated, and they are positively correlated with severity of diabetic nephropathy. PMID: 22088599
  78. positive expression of CD146 was associated with a poorer OS according to the survival analysis. CD146 may be a useful marker for predicting poor prognosis in patients with NSCLC following complete resection PMID: 22399604
  79. CD146 expression in vascular endothelial cells was significantly higher than that in invasive ductal carcinoma (IDC), and was positively correlated with tumor progression in invasive micropapillary carcinoma and IDC, not otherwise specified. PMID: 22051146
  80. elevated expression on CD4(+) T cells in autoimmune diseases PMID: 21959269
  81. It was shown that CD146 physically interacts with actin-linking ezrin-radixin-moesin proteins and recruits them to cell protrusions, promoting the formation and elongation of microvilli. PMID: 21725352
  82. METCAM/MUC18 expression increased in vitro motility, invasiveness, and anchorage-independent colony formation of SK-BR-3 cells and favored disorganized growth of the cells in 3D basement membrane culture PMID: 22057013
  83. this study suggested that the elevated expression of CD146, angiogenesis, and lymphangiogenesis might be closely related to progression, invasion, metastasis, and prognosis of gallbladder adenocarcinoma. PMID: 22076922
  84. promotes motility, invasion, and tumorigenesis of human breast cancer cells PMID: 21450088
  85. MUC18 is involved in cell signaling regulating the expression of Id-1 and ATF-3, thus contributing to melanoma metastasis PMID: 21467165
  86. novel findings show that the expression of CD146 differentiates between perivascular versus endosteal localization of non-hematopoietic bone marrow mesenchymal stem cell populations PMID: 21415267
  87. CD146 was mainly expressed in renal tubular epithelial cells, and might play an important role in the progression of IgA nephropathy. PMID: 20067117
  88. an allergic airway milieu (e.g., IL-13) increases MUC18 expression, which may contribute to increased bacterial infection/colonization in asthma and other lung diseases PMID: 21239604
  89. CD146 may be a key gene both in neoplasm invasion and blastocyst embedding because of its ability in regulating cell invasion. PMID: 21095067
  90. Enforced expression of human metastasis cell adhesion molecule/MUC18 increases prostate tumorigenesis in vivo and may affect the process by increasing proliferation PMID: 21334670
  91. Propose that CD146 is a sensitive and specific immunocytochemical marker enabling differential diagnosis of malignant pleural mesothelioma from reactive mesothelium. PMID: 20657552
  92. mRNA levels of CD31, CD144, CD146 and vWf in whole blood as detected by real time RT-PCR cannot be used as biomarkers for end-stage endothelial cells such as circulating endothelial cells. PMID: 20589320
  93. CD146 short isoform overexpression displays a distinct function in blood vessel regeneration. PMID: 20448216
  94. CD146 may serve as a marker of multipotency in human mesenchymal stem cells, with higher expression in tripotent vs. unipotent clonal populations. PMID: 20127798
  95. This study for the first time uncovered the pro-angiogenic role of CD146 and also pinpointed the key structural basis responsible for its signaling function and dimerization. PMID: 19782948
  96. Increased expression of MUC18 is implicated to play an important role in developing and malignant progression of human prostate cancer. PMID: 11722842
  97. The 28mer peptides are structural equivalents of the MAd18-5D7 epitope of Mel-CAM. PMID: 12406332
  98. MCAM and akt protein are reciprocally regulated. PMID: 14534536
  99. sCD146 circulates in the plasma of healthy subjects; significantly increased levels of sCD146 in blood and CD146 in kidney endothelial cells of patients with chronic renal failure were observed. PMID: 14597988
  100. endothelin-1 (ET-1) upregulates MCAM protein in primary human melanocytes PMID: 15610525

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Subcellular Location Membrane, Single-pass type I membrane protein
Tissue Specificity Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progress
Database Links

HGNC: 6934

OMIM: 155735

KEGG: hsa:4162

STRING: 9606.ENSP00000264036

UniGene: Hs.599039

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