Recombinant Human Cholinesterase(BCHE)

Code CSB-YP002604HU
Size US$1298
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names BCHE
Uniprot No. P06276
Research Area Neuroscience
Alternative Names Acylcholine acylhydrolase; BCHE; Butyrylcholine esterase; CHE1; CHE2; CHLE_HUMAN; Choline esterase II; Cholinesterase (serum) 2; Cholinesterase 1; Cholinesterase; E1; Pseudocholinesterase; Pseudocholinesterase E1
Species Homo sapiens (Human)
Source Yeast
Expression Region 29-602aa
Target Protein Sequence EDDIIIATKNGKVRGMNLTVFGGTVTAFLGIPYAQPPLGRLRFKKPQSLTKWSDIWNATKYANSCCQNIDQSFPGFHGSEMWNPNTDLSEDCLYLNVWIPAPKPKNATVLIWIYGGGFQTGTSSLHVYDGKFLARVERVIVVSMNYRVGALGFLALPGNPEAPGNMGLFDQQLALQWVQKNIAAFGGNPKSVTLFGESAGAASVSLHLLSPGSHSLFTRAILQSGSFNAPWAVTSLYEARNRTLNLAKLTGCSRENETEIIKCLRNKDPQEILLNEAFVVPYGTPLSVNFGPTVDGDFLTDMPDILLELGQFKKTQILVGVNKDEGTAFLVYGAPGFSKDNNSIITRKEFQEGLKIFFPGVSEFGKESILFHYTDWVDDQRPENYREALGDVVGDYNFICPALEFTKKFSEWGNNAFFYYFEHRSSKLPWPEWMGVMHGYEIEFVFGLPLERRDNYTKAEEILSRSIVKRWANFAKYGNPNETQNNSTSWPVFKSTEQKYLTLNTESTRIMTKLRAQQCRFWTSFFPKVLEMTGNIDEAEWEWKAGFHRWNNYMMDWKNQFNDYTSKKESCVGL
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 67.1kDa
Protein Length Full Length of Mature Protein
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters.
Gene References into Functions
  1. An increased frequency of the BChE K-allele in multiple sclerosis patients as compared to controls was found. PMID: 29358722
  2. This study validated preoperative serum BChE levels as an independent prognostic factor for urinary tract urothelial carcinoma after radical nephroureterectomy. PMID: 29177431
  3. we present and examine means of manipulating brain BChE levels by viral gene transfer, either regionally or globally, to modulate ghrelin signaling for long-term therapeutic purposes and to set the stage for exploring the neurophysiological impact of such an intervention. PMID: 28698452
  4. The BChE-K polymorphisms are associated with deleterious changes in cognitive decline in MCI patients treated with donepezil for 3 years. PMID: 27911294
  5. BCHE-K* positive subjects (and APOE-epsilon4) display an earlier age of onset of Alzheimer's Disease and an accelerated cognitive decline. PMID: 27567841
  6. Discovery of potent carbonic anhydrase, acetylcholinesterase, and butyrylcholinesterase enzymes inhibitors: The new amides and thiazolidine-4-ones synthesized on an acetophenone base.() PMID: 28544359
  7. HuBChE sequesters OP nerve agent in the bloodstream preventing the nerve agent from reaching critical target organ systems. HuBChE was effective when used as both a pre-treatment and as a post-exposure therapy. PMID: 28225154
  8. Significance of BChE Genetic Variants to Risk of Toxicity from Cholinesterase Inhibitors (review) PMID: 27551784
  9. Age, sex or smoking status did not influence butyrylcholinesterase activity in a healthy population. PMID: 28465191
  10. Mutations in the butyrylcholinesterase gene were associated with prolonged effect of succinylcholine or mivacurium. PMID: 27031121
  11. prolonged apnea after suxamethonium came about as a result of butyrylcholinesterase deficiency or mutation or acquired conditions leading to a decrease in the plasma cholinesterase activity PMID: 26439437
  12. the molecular mechanisms by which point mutations may lead to silent BChE variant or alter catalytic activity, is reported. PMID: 27062896
  13. BChE expression might be regulated by alpha-linolenic acid in HepG2 cells. PMID: 27106529
  14. Nine out of 18 (50%) individuals with butyrylcholinesterase activity below 2000 U/L had a mutation in 5'UTR (32G/A), intron 2 (c.1518-121T/C) or exon 4 (c.1699G/A; the K variant mutation). PMID: 27109752
  15. No significant difference in BChE levels of healthy pregnant women and high-risk pregnant women who had undergone a cesarean section under general anesthesia. PMID: 26955768
  16. Association between polymorphisms of BCHE and vitiligo in a two-step genetic association study. Upon confirmation of genetic association, the effect was investigated by a genotype-phenotype correlation experiment. PMID: 26189613
  17. Low serum Butyrylcholinesterase levels are associated with recurrence in prostate cancer. PMID: 26223693
  18. BCHE-K is associated with a reduced risk for Alzheimer disease and Lewy body dementia whereas APOEvarepsilon4 is associated with more rapid cognitive decline. PMID: 26757188
  19. Low BChE activity as a predictor of mortality in acute myocardial infarction might be related to its association with poor cardiac function. PMID: 27468571
  20. rs1803274 polymorphism of the BCHE gene represents a risk factor for in-stent restenosis after percutaneous coronary intervention. PMID: 26497592
  21. Complement component C3 and butyrylcholinesterase activity are associated with neurodegeneration and clinical disability in multiple sclerosis. PMID: 25835709
  22. BChE is a strong predictor for cardiac mortality specifically in younger patients with acute coronary syndrome aged between 45 and 64 years. PMID: 25933219
  23. High resolution melting analysis for the butyrylcholinesterase atypical variant genotyping is a simple, rapid, sensitive and low cost method PMID: 25336127
  24. There is a link between systemic inflammatory markers, oxidative stress, the PON192rs662-Q allele and BuChE-non-UU-phenotype and their corresponding enzymatic activity which may be considered as a risk factor for the severity of rheumatoid arthritis. PMID: 25179377
  25. There is a significant decrease in serum ChE activity after severe trauma PMID: 25471831
  26. This study proves that the p.Ala34Val BChE variant determines the "silent" phenotype (unable to hydrolyze succinylcholine and mivacurium). PMID: 25264279
  27. Data indicate that butyrylcholinesterase (BChE) was significantly associated with overall survival (OS). PMID: 24951323
  28. analysis of the T920C mutation allele frequency in butyrylcholinesterase gene in an Indian population PMID: 25447891
  29. Plasma arylesterase activity is positively associated with plasma ChE specific activity in a nested case-control study. PMID: 24473115
  30. BChE-K carriers are protected from the pathological detriments of Alzheimer's disease that affect cortical thickness. PMID: 24479631
  31. results confirm the association of APOE with Abeta deposition and represent the largest known effect of BCHE on an AD-related phenotype. PMID: 23419831
  32. Results indicate that BuChE non-UU phenotype and PON 55M allele are significant risk factors for systemic lupus erythematosus. PMID: 24399815
  33. Our findings suggest that the AA genotype of rs1803274 is a risk factor for crack cocaine use, which is more addictive than powder cocaine use. PMID: 24312228
  34. We report the case of a 65-year-old patient who was diagnosed with butyrylcholinesterase resulting in prolonged paralysis and extended mechanical ventilation PMID: 24446003
  35. Two mutants of human BCHE can effectively eliminate (-)-cocaine, cocaethylene and norcocaine in simplified kinetic models of cocaine abuse and overdose associated with the concurrent use of cocaine and alcohol. PMID: 24870023
  36. BChE activity might be involved in the pathogenesis of preeclampsia through influence on lipid and lipoprotein metabolism and oxidative stress. PMID: 23650977
  37. The present data are still inconclusive with respect to a possible contribution of common genetic variants in BCHE to the pathogenesis of attention-deficit/hyperactivity syndrome. PMID: 24041656
  38. CocH3 (BCHE mutant) with an identical concentration with that of the endogenous BChE in human plasma can effectively eliminate both cocaine and norcocaine in a simplified kinetic model of cocaine abuse. PMID: 24125115
  39. BCHE 1914G allele showed higher frequency in the obese group. Carriers of 1914G BCHE allele showed lower mean BChE activity.BCHE 1914G allele is influencing physiological mechanisms related to obesity. PMID: 24001779
  40. Data indicate that high AChE affinity of the compounds was achieved by optimizing different substituents on the pyridazinone ring, without sacrificing the AChE/BuChE selectivity profile. PMID: 23466605
  41. Water in the active site gorge of the D70G mutant is more easily depleted than that in wild-type BChE. PMID: 23782236
  42. These findings show that BCHE can hydrolyze 2-Arachidonoylglycerol which may be evidence of a more specific role for BCHE in endocannabinoid regulation. PMID: 23689009
  43. This review examines the roles of gender and BuChE genotype in the phenotypic expression of Alzheimer disease[review] PMID: 22402324
  44. Elevated levels of BuChE observed in active multiple sclerosis (MS) lesions could be related to the decompaction of myelin characteristic of the disorder. PMID: 22778864
  45. [review] Butyrylcholinesterase has diverged both structurally and in terms of tissue and cellular expression patterns from acetylcholinesterase. PMID: 22750491
  46. aspirin is hydrolyzed in plasma by two enzymes, BChE and a new extracellular form of platelet-activating factor acetylhydrolase, PAFAH1b2. PMID: 23508960
  47. characterized the catalytic activities of wild-type BChE and the A199S/F227A/S287G/A328 W/Y332G mutant against both (+)- and (-)-cocaine at the same time under the same experimental conditions PMID: 22917637
  48. verify amplification and/or deletion in the ACHE, BCHE, EPHB4 and MME genes in 32 samples of sporadic breast cancer PMID: 23063927
  49. performed molecular dynamics simulation to probe the structural stability of Indian variant (L307P) in comparison with wild and other BChE variants (D70G, E497V, V142M) having differential esterase activity PMID: 23123771
  50. Alkylating molecules were synthesized and the crystal structures they form with soman-aged butyrylcholinesterase were solved. PMID: 22922115
  51. protective effect of -116A and K variants on juvenile obesity risk, suggesting a role for the BCHE gene on juvenile onset obesity different from that observed on adult onset obesity PMID: 23000450
  52. The allele, genotype and haplotype frequencies of the K and the -116A variants of BCHE gene were not significantly different between Alzheimer disease cases and controls. PMID: 23022600
  53. Radioligands targeting unusual BuChE activity in the brain may represent a means for early diagnosis and treatment monitoring of Alzheimer disease. PMID: 22935510
  54. designed three BChE peptides by deleting some N- and C-terminal residues of BChE by maintaining the folds of the active site core that includes the three active site residues (S198, E325, and H438) PMID: 23044488
  55. reaction mechanisms and free energy profiles for spontaneous reactivation of wild-type BChE and its G117H mutant phosphorylated by sarin have been explored by performing first-principles quantum mechanical/molecular mechanical free energy calculations; the role of the G117H mutation on the activity has been elucidated PMID: 23092211
  56. peripheral site of human butyrylcholinesterase PMID: 22901043
  57. AChE and BChE activities were decreased in prostate cancer patients PMID: 22560633
  58. BCHE may be involved in the pathogenesis of hepatitis C-related fibrosis among injection drug users. PMID: 22331678
  59. In vitro analysis indicated that high ApoE protein levels were related to an increased pool of dormant BuChE molecules with an abnormally high intrinsic catalytic rate in cerebrospinal fluid, which is "turned on" by excess amyloid beta peptides PMID: 20538374
  60. Data conclude that variation in BCHE activity is due to multiple variants across the spectrum from uncommon/large effect to common/small effect, and partly results from (rather than causes) metabolic abnormalities. PMID: 21862451
  61. ApoE epsilon4 might impart its pathological role through high protein expression and interaction with butyrylcholinesterase, which in turn might modulate central cholinergic activity and amyloidbeta load in the brain. PMID: 19713000
  62. Serum ChE might be a predictive marker for the prognosis of aged patients with systemic inflammatory response syndrome, with low serum ChE levels indicating poor prognosis. PMID: 22040426
  63. Compared with controls, bipolar patients had a significantly higher pseudocholinesterase activity. PMID: 22294139
  64. In moderate-to-severe AD (MMSE PMID: 20644562
  65. Butyrylcholinesterase is associated with a subpopulation ofbeta-amyloid plaques and may play a role in Alzheimer's disease plaque maturation. PMID: 22157615
  66. Data show that BChE interacts with amylin through its core domain and efficiently attenuates both amylin fibril and oligomer formation. PMID: 20807286
  67. Low plasma butyrylcholinesterase activity can be a useful candidate biomarker to identify subjects at greater risk of developing postoperative delirium. PMID: 21576115
  68. Despite the geographical coexistence throughout their dispersion, each of these two Amerindian groups retains a high degree of genetic identity, probably maintained by cultural and social isolation. PMID: 21547979
  69. Glucose as an independent risk factor for higher BuChE activity in females indicates that BuChE may be a predictor of diabetes. PMID: 21402063
  70. allelic and haplotype variations in BCHE genes were associated with changes in blood BChE activities; BChE activity was associated with features of the metabolic syndrome PMID: 21493754
  71. In this case, an at limits significant result (p=0.03) was obtained for BChE, whose A allele (the K variant) in patients was found associated with higher plasma concentrations of high density lipoprotein-cholesterol. PMID: 21473860
  72. significant differences in kinetic pattern and K(i) values between recombinant wild type BChE and F329A mutant suggest that phenylalanine at position 329 in BChE is a critical residue in malachite green and methyl green binding to enzyme PMID: 21530486
  73. BChE reacts preferentially with P(R) enantiomer of V-agents and does not age, in complete contrast to AChE, which is selectively inhibited by the P(S) enantiomer and ages PMID: 21454498
  74. eleven of 13 patients with a prolonged duration of action of succinylcholine had mutations in BCHE, indicating that this is the possible reason for a prolonged period of apnea PMID: 21029050
  75. Data show that kidney and renal tumours showed comparable levels of acetylcholinesterase mRNA, the content of butyrylcholinesterase mRNA was increased 10-fold in pRCC. PMID: 20883446
  76. mutation-caused shifts of the free energy change from the free enzyme to the rate-determining transition state for (-)-cocaine hydrolysis PMID: 20677742
  77. mutations on certain nonactive site residues can indirectly affect the catalytic efficiency of the enzyme against (-)-cocaine by enhancing or weakening the overall hydrogen bonding between the oxygen of (-)-cocaine and the oxyanion hole of the enzyme PMID: 20886866
  78. Pseudocholinesterase deficiency is an inherited or acquired condition in which the metabolism of succinylcholine, mivacurium, or ester local anesthetics is potentially impaired PMID: 20879632
  79. molecular dynamics (MD) simulations on wild-type BuChE (PDB ID: 1P0I) and several known and new variants of this enzyme PMID: 20399202
  80. Surgical pieces of prostatic cancer (PC) and benign prostatic hyperplasia (BPH) were analyzed for AChE and BChE activity. PMID: 20356562
  81. alterations in BuChE activity in multiple sclerosis white matter lesions PMID: 20122907
  82. The concomitant presences of two alleles of the BCHE-K and APOE-epsilon 4 in increasing the risk of coronary artery disease (CAD) or diabetes in type 2 diabetes mellitus (T2DM) patients with or without CAD and CAD patients without T2DM. PMID: 19685167
  83. observed differences in frequency of the BuChE K variant in Alzheimer's disease (AD) and fronto-temporal dementia (FTD) supports the evidence of different molecular pathways involved in the pathogenesis of AD and FTD. PMID: 20058037
  84. may be involved in reducing oxidative stress in diabetic pregnancy PMID: 20143968
  85. The majority of the breast tumor tissues showed a notable number of both deletions and amplifications: 65.7% and 22.9%, respectively, in BCHE and 45.7% and 31.4%, respectively, in ACHE. PMID: 20193849
  86. Mutations associated with prolonged neuromuscular block during anesthesia. PMID: 11749053
  87. Butyrylcholinesterase K variant on chromosome 3 q is associated with Type II diabetes in white Caucasian subjects. PMID: 11793025
  88. can hydrolyze acetylcholine in the normal and Alzheimer brain. PMID: 11848688
  89. Engineering of a monomeric and low-glycosylated form PMID: 11856322
  90. Human butyrylcholinesterase is reversibly inhibited by tetraethylammonium ion. PMID: 11856351
  91. serum enzyme inhibited by antidepressants, fluoxetine, sertraline and amitriptyline, in a dose related manner PMID: 12009429
  92. BuChE-catalyzed hydrolysis of N-methylindoxyl acetate: analysis of volume changes upon reaction and hysteretic behavior. Hydrolysis by wild-type BuChE and peripheral site mutants (D70G, Y332A, D70G/Y332A) was found to follow Michaelis-Menten kinetics. PMID: 12044901
  93. There is a minor association between BChE-K and early-onset coronary artery disease, especially in the presence of the APOE-epsilon 4 allele PMID: 12074828
  94. Wild-type and A328W mutant expressed in Chinese hamster ovary cells have a 16-hour half-life in the circulation and protect mice from cocaine toxicity. PMID: 12130740
  95. Novel mutation and multiple mutations found in the human butyrylcholinesterase gene affect enzyme activity. PMID: 12417112
  96. Butyrylcholinesterase is a major regulator of attention especially in cholinergic deficiency states through its ability to hydrolyse acetylcholine. PMID: 12668920
  97. Data help to better define the etiology of low BChE activity and the role of the rather common K allele PMID: 12724618
  98. The distinct distribution of BuChE activity in neurons in the human thalamus is consistent with an important role for this enzyme in neurotransmission in the human nervous system. PMID: 12811800
  99. the structure of BChE is similar to a previously published theoretical model of this enzyme and to the structure of Torpedo acetylcholinesterase PMID: 12869558
  100. replacement of Ala277(Trp279) with Trp in human BChE does not affect the binding of E2020 to BChE PMID: 14622273

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Involvement in disease Butyrylcholinesterase deficiency (BChE deficiency)
Subcellular Location Secreted
Protein Families Type-B carboxylesterase/lipase family
Tissue Specificity Detected in blood plasma (at protein level). Present in most cells except erythrocytes.
Database Links

HGNC: 983

OMIM: 177400

KEGG: hsa:590

STRING: 9606.ENSP00000264381

UniGene: Hs.420483

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