Recombinant Human DNA-directed RNA polymerase I subunit RPA43 (TWISTNB)

Code CSB-YP658940HU
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Source Yeast
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Code CSB-EP658940HU
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Source E.coli
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Code CSB-EP658940HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP658940HU
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Source Baculovirus
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Code CSB-MP658940HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TWISTNB
Uniprot No.
Alternative Names
DNA directed RNA polymerase I subunit RPA43; DNA-directed RNA polymerase I subunit RPA43; RPA43; RPA43_HUMAN; TWIST neighbor; Twist neighbor protein; Twistnb
Species
Homo sapiens (Human)
Expression Region
1-338
Target Protein Sequence
MAAGCSEAPR PAAASDGSLV GQAGVLPCLE LPTYAAACAL VNSRYSCLVA GPHQRHIALS PRYLNRKRTG IREQLDAELL RYSESLLGVP IAYDNIKVVG ELGDIYDDQG HIHLNIEADF VIFCPEPGQK LMGIVNKVSS SHIGCLVHGC FNASIPKPEQ LSAEQWQTME INMGDELEFE VFRLDSDAAG VFCIRGKLNI TSLQFKRSEV SEEVTENGTE EAAKKPKKKK KKKDPETYEV DSGTTKLADD ADDTPMEESA LQNTNNANGI WEEEPKKKKK KKKHQEVQDQ DPVFQGSDSS GYQSDHKKKK KKRKHSEEAE FTPPLKCSPK RKGKSNFL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Through its association with RRN3/TIF-IA may be involved in recruitment of Pol I to rDNA promoters.
Gene References into Functions
  1. describe the localisation and characterisation of the TWIST NEIGHBOR (TWISTNB) gene PMID: 12438708
Subcellular Location
Nucleus, nucleolus.
Protein Families
Eukaryotic RPA43 RNA polymerase subunit family
Tissue Specificity
Widely expressed. Expressed in all fetal and adult tissues tested, with highest expression in fetal lung, liver, and kidney, and low expression in all adult tissues.
Database Links

HGNC: 18027

OMIM: 608312

KEGG: hsa:221830

STRING: 9606.ENSP00000222567

UniGene: Hs.353035

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