Recombinant Human DNA fragmentation factor subunit beta (DFFB)

1. we show that executioner caspase activation of the apoptotic nuclease CAD/DFF40 is essential for TRAIL-induced mutations in surviving cells. As exposure to chemotherapy drugs also activates apoptotic caspases and presumably CAD, we hypothesized that these pathways may also contribute to the mutagenesis induced by conventional chemotherapy drugs, perhaps augmenting the mutations that arise from direct DNA damage PMID: 28981092
2. Dff40 expression is upregulated in atherosclerotic plaque. PMID: 28007744
3. the low expression levels of DFF40/CAD and the absence of DNA laddering as common molecular traits in glioblastoma PMID: 26755073
4. Data suggest DFF40 expression in breast cancer cell line is involved in drug sensitivity/resistance to doxorubicin; apoptotic cell death due to doxorubicin (a topoisomerase II inhibitor) is enhanced by DFF40 overexpression in breast cancer cell line. PMID: 26529233
5. Combinatorial use of some sulfonamides such as acetazolamide along with increased expression of DFF40 can potently kill tumor cells via apoptosis. PMID: 25086620
6. DFF40/CAD-independent mechanism driving conformational nuclear changes during caspase-dependent cell death PMID: 24838313
7. the highest order of chromatin compaction observed in the later steps of caspase-dependent apoptosis relies on DFF40/CAD-mediated DNA damage by generating 3'-OH ends in single-strand rather than double-strand DNA nicks/breaks PMID: 23430749
8. These results suggest a cooperative activity between CAD and DNAS1L3 to accomplish internucleosomal DNA fragmentation . PMID: 23229555
9. Human papillomavirus type 16 E6 protein inhibits DNA fragmentation via interaction with DNA fragmentation factor 40 PMID: 22609799
10. During apoptotic rearrangement of interchromatin granule clusters, the nuclear matrix (NuMa rearrangement) and chromatin are closely associated. This process occurs in defined stages and depends on the activity of protein phosphatases, caspases and CAD. PMID: 22023725
11. the cytosolic levels of DFF40/CAD are determinants in achieving a complete apoptotic phenotype, including oligonucleosomal DNA degradation. PMID: 22253444
12. Data show that among the 13 SNPs in the 3 genes, only 3 were found to be polymorphic: R196K and K277R in the DFFB gene, and S12L in the EndoG gene, and all 6 SNPs in the FEN-1 gene were entirely monoallelic. PMID: 22011247
13. These data suggest that DFF 40 mediated apoptosis plays a significant role in mediating sepsis induced cellular dysfunction. PMID: 21820410
14. Mutations and aberrantly spliced transcripts for the CAD gene are frequently associated with hepatocellular carcinoma. PMID: 12610505
15. Hsp70 binds free CAD in TCR-stimulated T cells to stabilize and augment its activity. PMID: 12738667
16. CAD involves unrestricted accessibility of chromosomal DNA at the initial phase of apoptosis, followed by its nuclear immobilization that may prevent the release of the active nuclease into the extracellular environment. PMID: 15569712
17. PARP-1 poly(ADP-ribosyl)ation is a terminal event in the apoptotic response that occurs in response to DNA fragmentation and directly influences DFF40 activity PMID: 15703174
18. Interactions identified here between human placenta histone H1 carboxyl-terminal domain and DFF40/CAD target and activate linker DNA cleavage during the terminal stages of apoptosis. PMID: 15910001
19. Our findings of high frequency of Alu-mediated hCAD deletion in human hepatoma underscore the implication of hCAD in hepatocarcinogenesis PMID: 16007181
20. AIF is responsible for stage I nuclear morphology and HMW DNA degradation is a caspase-activated DNase and AIF-independent process PMID: 16049016
21. levels of CAD were significantly higher in the nuclear fraction of temporal lobe epilepsy samples PMID: 16121124
22. DFFB haploinsufficiency from 1p allelic loss is a contributing factor in oligodendroglioma development PMID: 16156899
23. in apoptotic cells, endogenous and exogenous CAD forms limited oligomers, representing the active nuclease PMID: 16204257
24. the N-terminal region was found to be responsible for the requirement of salt for fibril formation PMID: 16428311
25. CAD is downregulated at the mRNA and protein level during the erythroid differentiation in TF-1 cells. PMID: 16529748
26. poly-glutamic acid and heparin inhibit DFF40/CAD, the latter one being highly effective at nanomolar concentrations. The inhibitory poly-anions bind to the nuclease and impair its ability to bind double-stranded DNA. PMID: 16699957
27. These results suggest that CAD is the endogenous endonuclease that mediates internucleosomal DNA degradation in rotenone-induced apoptosis. PMID: 17239993
28. Data suggest that erythroblast chromatin degradation may involve caspase activated DNase and apoptosis inducing factor, enzymes distinct from those active in apoptotic cells. PMID: 17492772
29. changes induced in DNA conformation upon HMG-box binding makes the substrate more accessible to cleavage by DFF40/CAD nuclease and thus may contribute to preferential linker DNA cleavage during apoptosis PMID: 18239742
30. We have found that neither single-stranded DNA, single-stranded RNA, double-stranded RNA nor RNA-DNA heteroduplexes are cleaved by the DFF40/CAD nuclease. PMID: 18283539

Show More

Hide All

HGNC: 2773

OMIM: 601883

KEGG: hsa:1677

STRING: 9606.ENSP00000367454

UniGene: Hs.133089