Recombinant Human DNA repair and recombination protein RAD54B (RAD54B), partial

Code CSB-YP896748HU
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Source Yeast
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Code CSB-EP896748HU
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Source E.coli
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Code CSB-EP896748HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP896748HU
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Source Baculovirus
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Code CSB-MP896748HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RAD54B
Uniprot No.
Alternative Names
RAD54B; DNA repair and recombination protein RAD54B; EC 3.6.4.-; RAD54 homolog B
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in DNA repair and mitotic recombination. May play an active role in recombination processes in concert with other members of the RAD52 epistasis group.
Gene References into Functions
  1. High RAD54B expression is associated with hepatoma. PMID: 29956808
  2. Review of structural aspects of RAD54B, molecular functions associated with its cellular roles in preventing genome instability, and how aberrant function contributes to oncogenesis. PMID: 28295846
  3. RAD54B expression has potential to serve as a novel prognostic biomarker, particularly for distant recurrence in colorectal cancer PMID: 26046797
  4. Patients with expression of both FEN1 and RAD54B were prone to have advanced nodal involvement and significantly poor prognosis. PMID: 26431531
  5. The scaffolding function of Rad54B dynamically regulates the maintenance of genome integrity by limiting checkpoint strength. PMID: 25384516
  6. support a model in which RAD54L and RAD54B counteract genome-destabilizing effects of direct binding of RAD51 to dsDNA in tumor cells PMID: 25765654
  7. RAD54B-deficient cells are selectively killed relative to controls via siRNA-based silencing of SOD1 PMID: 24002644
  8. RAD54B and GREB1 gene polymorphisms may not be associated with PCOS in the Han Chinese population. PMID: 23876972
  9. These results suggest that hINO80 assists double-strand break repair by positively regulating the expression of the Rad54B and XRCC3 genes. PMID: 20687897
  10. Rad54B is a DNA-dependent ATPase and has biochemical properties different from its structural homolog in yeast, Tid1/Rdh54 PMID: 11884632
  11. Rad54B enhanced the DNA strand-exchange activity of Dmc1 by stabilizing the Dmc1-single-stranded DNA complex. PMID: 16945962
  12. the loss of heterozygosity (LOH) in the 8q12-q24.1 chromosomal region, containing RAD54B gene isn't predictive for breast cancer PMID: 17585536
  13. S100A11 targets Rad54B to sites of DNA DSB repair sites and has a role in p21-based regulation of cell cycle PMID: 18463164
  14. RAD54B binding may affect the quaternary structure of DMC1. PMID: 18718930
  15. RAD54B-deficient human colorectal cancer cells are sensitive to synthetic lethal killing by reduced FEN1 expression, while isogenic RAD54B proficient cells are not PMID: 19218431

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Subcellular Location
Nucleus.
Protein Families
SNF2/RAD54 helicase family
Tissue Specificity
Abundantly expressed in testis and spleen. Relatively low levels observed in thymus, prostate, ovary and colon.
Database Links

HGNC: 17228

OMIM: 604289

KEGG: hsa:25788

STRING: 9606.ENSP00000336606

UniGene: Hs.30561

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