Recombinant Human Dihydroxyacetone phosphate acyltransferase (GNPAT)

Code CSB-YP009629HU
MSDS
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Source Yeast
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Code CSB-EP009629HU
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Source E.coli
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Code CSB-EP009629HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP009629HU
MSDS
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Source Baculovirus
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Code CSB-MP009629HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GNPAT
Uniprot No.
Alternative Names
Acyl CoA dihydroxyacetonephosphateacyltransferase ; Acyl-CoA:dihydroxyacetonephosphateacyltransferase; DAP AT; DAP-AT; DAPAT; DHAP AT ; DHAP-AT; DHAPAT; Dihydroxyacetone phosphate acyltransferase; EC 2.3.1.42 ; Glycerone-phosphate O-acyltransferase; Glyceronephosphate O acyltransferase; GNPAT; GNPAT_HUMAN; OTTHUMP00000036147
Species
Homo sapiens (Human)
Expression Region
1-680
Target Protein Sequence
MESSSSSNSY FSVGPTSPSA VVLLYSKELK KWDEFEDILE ERRHVSDLKF AMKCYTPLVY KGITPCKPID IKCSVLNSEE IHYVIKQLSK ESLQSVDVLR EEVSEILDEM SHKLRLGAIR FCAFTLSKVF KQIFSKVCVN EEGIQKLQRA IQEHPVVLLP SHRSYIDFLM LSFLLYNYDL PVPVIAAGMD FLGMKMVGEL LRMSGAFFMR RTFGGNKLYW AVFSEYVKTM LRNGYAPVEF FLEGTRSRSA KTLTPKFGLL NIVMEPFFKR EVFDTYLVPI SISYDKILEE TLYVYELLGV PKPKESTTGL LKARKILSEN FGSIHVYFGD PVSLRSLAAG RMSRSSYNLV PRYIPQKQSE DMHAFVTEVA YKMELLQIEN MVLSPWTLIV AVLLQNRPSM DFDALVEKTL WLKGLTQAFG GFLIWPDNKP AEEVVPASIL LHSNIASLVK DQVILKVDSG DSEVVDGLML QHITLLMCSA YRNQLLNIFV RPSLVAVALQ MTPGFRKEDV YSCFRFLRDV FADEFIFLPG NTLKDFEEGC YLLCKSEAIQ VTTKDILVTE KGNTVLEFLV GLFKPFVESY QIICKYLLSE EEDHFSEEQY LAAVRKFTSQ LLDQGTSQCY DVLSSDVQKN ALAACVRLGV VEKKKINNNC IFNVNEPATT KLEEMLGCKT PIGKPATAKL
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Dihydroxyacetonephosphate acyltransferase involved in plasmalogen biosynthesis.
Gene References into Functions
  1. GNPAT p.D519G is strongly associated with markedly increased iron stores in p.C282Y homozygotes after correction for age, iron-related variables, and alcohol consumption. PMID: 27936396
  2. GNPAT rs11558492 is not a major modifier of iron status and is not associated with liver fibrosis in HFE-related Hemochromatosis patients. PMID: 28425416
  3. Here, we report the characterization of the recombinant human DHAP acyl-transferase, which performs the first step in alkyl-DHAP synthesis. PMID: 27836547
  4. Reduction of GNPAT activated NF-kappaB in glial cell lines and microglia in cortex. PMID: 28292831
  5. C282Y homozygotes referred for HFE testing commonly have a GNPAT variant. This GNPAT variant does not appear be a co-modifying gene affecting expression of HFE related hemochromatosis in this population. The GNPAT variant does not predict the severity of iron overload. PMID: 27740525
  6. The variant of the GNPAT gene showed the most significant association with severe iron overload. PMID: 25605615
  7. ACOX1 and GNPAT silencing up-regulated ceramide galactosyltransferase (UGT8) mRNA expression, and down-regulated UDP-glucoseceramide glucosyltransferase (UGCG). PMID: 23933200
  8. Novel mutations in GNPAT cause rhizomelic chondrodysplasia punctata (RCDP) type 2. PMID: 21990100
  9. peroxisomal DHAPAT is essential for the biosynthesis of plasmalogens in animal cells PMID: 15687349
  10. Chromosome 1q42.1 harbors GNPAT and DISC1 as candidate genes for schizophrenia. PMID: 16997000

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Involvement in disease
Rhizomelic chondrodysplasia punctata 2 (RCDP2)
Subcellular Location
Peroxisome membrane; Peripheral membrane protein; Matrix side.
Protein Families
GPAT/DAPAT family
Database Links

HGNC: 4416

OMIM: 222765

KEGG: hsa:8443

STRING: 9606.ENSP00000355607

UniGene: Hs.498028

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