Recombinant Human E3 ubiquitin-protein ligase ARIH1 (ARIH1)

Code CSB-YP896897HU
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Source Yeast
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Code CSB-EP896897HU
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Source E.coli
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Code CSB-EP896897HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP896897HU
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Source Baculovirus
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Code CSB-MP896897HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ARIH1
Uniprot No.
Alternative Names
ARI 1; ARI; ARI-1; ARI1; ARI1_HUMAN; Ariadne Drosophila homolog of; Ariadne homolog; Ariadne homolog ubiquitin conjugating enzyme E2 binding protein 1; Ariadne ubiquitin conjugating enzyme E2 binding protein homolog 1; ARIH 1; ARIH1; E3 ubiquitin protein ligase ARIH1; H7 AP2; H7-AP2; HARI; HHARI; human homologue of ariadne ; Monocyte protein 6; MOP 6; MOP-6; MOP6; Protein ariadne 1 homolog; Protein ariadne-1 homolog; Protein ariadne1 homolog; UbcH7 binding protein; UBCH7 BP; UbcH7-binding protein; UBCH7BP; UbcM4 interacting protein; UbcM4-interacting protein; Ubiquitin conjugating enzyme E2 binding protein 1; Ubiquitin-conjugating enzyme E2-binding protein 1
Species
Homo sapiens (Human)
Expression Region
1-557
Target Protein Sequence
MDSDEGYNYE FDEDEECSEE DSGAEEEEDE DDDEPDDDTL DLGEVELVEP GLGVGGERDG LLCGETGGGG GSALGPGGGG GGGGGGGGGG PGHEQEEDYR YEVLTAEQIL QHMVECIREV NEVIQNPATI TRILLSHFNW DKEKLMERYF DGNLEKLFAE CHVINPSKKS RTRQMNTRSS AQDMPCQICY LNYPNSYFTG LECGHKFCMQ CWSEYLTTKI MEEGMGQTIS CPAHGCDILV DDNTVMRLIT DSKVKLKYQH LITNSFVECN RLLKWCPAPD CHHVVKVQYP DAKPVRCKCG RQFCFNCGEN WHDPVKCKWL KKWIKKCDDD SETSNWIAAN TKECPKCHVT IEKDGGCNHM VCRNQNCKAE FCWVCLGPWE PHGSAWYNCN RYNEDDAKAA RDAQERSRAA LQRYLFYCNR YMNHMQSLRF EHKLYAQVKQ KMEEMQQHNM SWIEVQFLKK AVDVLCQCRA TLMYTYVFAF YLKKNNQSII FENNQADLEN ATEVLSGYLE RDISQDSLQD IKQKVQDKYR YCESRRRVLL QHVHEGYEKD LWEYIED
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3. Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-RING ubiquitin ligase (CRL) complexes and initiating ubiquitination of CRL substrates: associates with CRL complexes and specifically mediates addition of the first ubiquitin on CRLs targets. The initial ubiquitin is then elongated by CDC34/UBE2R1 and UBE2R2. E3 ubiquitin-protein ligase activity is activated upon binding to neddylated cullin-RING ubiquitin ligase complexes. Plays a role in protein translation in response to DNA damage by mediating ubiquitination of EIF4E2, the consequences of EIF4E2 ubiquitination are however unclear. According to a report, EIF4E2 ubiquitination leads to promote EIF4E2 cap-binding and protein translation arrest. According to another report EIF4E2 ubiquitination leads to its subsequent degradation. Acts as the ligase involved in ISGylation of EIF4E2. In vitro, controls the degradation of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex member SUN2 and may therefore have a role in the formation and localization of the LINC complex, and as a consequence, nuclear subcellular localization and nuclear morphology.
Gene References into Functions
  1. ARIH1-mediated mitophagy promotes therapeutic resistance. PMID: 28930681
  2. Variants in ARIH1 are associated with developing aortic aneurysms. PMID: 29689197
  3. Crystal structure of HHARI and UbcH7 reveals UbcH7 approximately Ub binding RING1 domain of auto-inhibited HHARI.HHARI UBA-L domain has Ub binding properties. PMID: 28552575
  4. HHARI prevents spurious discharge of Ub from E2 to lysine residues by harboring structural elements that block E2 ~ Ub from adopting a 'closed' conformation. PMID: 28790309
  5. DNA damage induces an increase in ARIH1 protein levels and association of ARIH1 with 4EHP. In turn, this causes 4EHP recruitment to the mRNA cap, where it is known to compete with eIF4E. PMID: 25624349
  6. Determined is the three-dimensional solution structure of the catalytic RING2 domain from HHARI. It shows glimpses of a HECT E3 ligase. PMID: 24058416
  7. HHARI is a marker of cellular proliferation associated with nuclear bodies. PMID: 23059369
  8. Both parkin and HHARI bind the same substrate proteins in cells and induce the formation of aggresomes. PMID: 21590270
  9. UBCH7 exhibits activity with the RING-in-between-RING (RBR) family of E3s that includes parkin (also known as PARK2) and human homologue of ariadne (HHARI; also known as ARIH1) PMID: 21532592
  10. the structure of the RING2 from the RING-IBR-RING motif of HHARI and showed that RING2 has a completely distinct topology from classical RINGs PMID: 15236971

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Subcellular Location
Cytoplasm. Nucleus. Nucleus, Cajal body.
Protein Families
RBR family, Ariadne subfamily
Tissue Specificity
Widely expressed.
Database Links

HGNC: 689

OMIM: 605624

KEGG: hsa:25820

STRING: 9606.ENSP00000369217

UniGene: Hs.268787

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