Recombinant Human Enoyl-CoA delta isomerase 1, mitochondrial (ECI1)

Code CSB-YP006546HU
MSDS
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Source Yeast
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Code CSB-EP006546HU
MSDS
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Source E.coli
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Code CSB-EP006546HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP006546HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP006546HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ECI1
Uniprot No.
Alternative Names
2-trans-enoyl-CoA isomerase; 3; 3,2 trans enoyl Coenzyme A isomerase ; 3,2 trans-enoyl-CoA isomerase; 3,2-trans-enoyl-CoA isomerase; mitochondrial; Acetylene allene isomerase; D2-enoyl-CoA isomerase; D3; D3,D2 enoyl CoA isomerase ; DCI; DCI protein ; delta(2)-enoyl-CoA isomerase; Delta(3); Delta(3),Delta(2) enoyl CoA isomerase ; Delta3; delta2 enoyl CoA isomerase; Dodecenoyl CoA delta Isomerase; Dodecenoyl CoA isomerase; Dodecenoyl Coenzyme A delta isomerase ; Dodecenoyl Coenzyme A delta isomerase (3,2 trans enoyl Coenzyme A isomerase); Dodecenoyl Coenzyme A delta isomerase 3,2 trans enoyl Coenzyme A isomerase; isoform CRA a; Dodecenoyl-CoA isomerase; ECI1; ECI1_HUMAN; Enoyl-CoA delta isomerase 1; enoyl-CoA delta isomerase 1; mitochondrial; mitochondrial
Species
Homo sapiens (Human)
Expression Region
42-302
Target Protein Sequence
FGSQRVLVE PDAGAGVAVM KFKNPPVNSL SLEFLTELVI SLEKLENDKS FRGVILTSDR PGVFSAGLDL TEMCGRSPAH YAGYWKAVQE LWLRLYQSNL VLVSAINGAC PAGGCLVALT CDYRILADNP RYCIGLNETQ LGIIAPFWLK DTLENTIGHR AAERALQLGL LFPPAEALQV GIVDQVVPEE QVQSTALSAI AQWMAIPDHA RQLTKAMMRK ATASRLVTQR DADVQNFVSF ISKDSIQKSL QMYLERLKEE KG
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species.
Gene References into Functions
  1. Hepatitis C virus growth and RNA replication in hepatoma cell lines stably expressing coenzyme A delta isomerase (DCI)-targeting short hairpin RNA (shRNA) were abrogated, indicating that DCI is required for productive infection. PMID: 21917952
  2. determination of structure and mode of binding for fatty acyl group PMID: 15351645
Subcellular Location
Mitochondrion matrix.
Protein Families
Enoyl-CoA hydratase/isomerase family
Tissue Specificity
Expressed in liver (at protein level).
Database Links

HGNC: 2703

OMIM: 600305

KEGG: hsa:1632

STRING: 9606.ENSP00000301729

UniGene: Hs.403436

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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