Recombinant Human GTP-binding protein Rhes (RASD2)

Code CSB-YP842653HU
MSDS
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Source Yeast
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Code CSB-EP842653HU
MSDS
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Source E.coli
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Code CSB-EP842653HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP842653HU
MSDS
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Source Baculovirus
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Code CSB-MP842653HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RASD2
Uniprot No.
Alternative Names
GTP binding protein Rhes; GTP-binding protein Rhes; MGC:4834; OTTHUMP00000197925; Ras homolog enriched in striatum; RASD 2; RASD family member 2; RASD2; Rhes; RHES_HUMAN; TEM 2; TEM2; Tumor endothelial marker 2
Species
Homo sapiens (Human)
Expression Region
1-263
Target Protein Sequence
MMKTLSSGNC TLSVPAKNSY RMVVLGASRV GKSSIVSRFL NGRFEDQYTP TIEDFHRKVY NIRGDMYQLD ILDTSGNHPF PAMRRLSILT GDVFILVFSL DNRESFDEVK RLQKQILEVK SCLKNKTKEA AELPMVICGN KNDHGELCRQ VPTTEAELLV SGDENCAYFE VSAKKNTNVD EMFYVLFSMA KLPHEMSPAL HRKISVQYGD AFHPRPFCMR RVKEMDAYGM VSPFARRPSV NSDLKYIKAK VLREGQARER DKC
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
GTPase signaling protein that binds to and hydrolyzes GTP. Regulates signaling pathways involving G-proteins-coupled receptor and heterotrimeric proteins such as GNB1, GNB2 and GNB3. May be involved in selected striatal competencies, mainly locomotor activity and motor coordination.
Gene References into Functions
  1. Study demonstrated that variation in the gene coding for RASD2 (rs6518956) affects in vivo prefrontal and striatal phenotypes in healthy human subjects that are relevant to schizophrenia PMID: 26228524
  2. Rhes influences striatal cAMP/PKA-dependent signaling and synaptic plasticity in a gender-sensitive fashion PMID: 26190541
  3. Findings reveal that ras homolog enriched in striatum is localized also in striatal cholinergic interneurons and, most importantly, lack of this G-protein, significantly alters dopamine D2 receptor modulation of striatal cholinergic excitability. PMID: 25818655
  4. Rhes is reduced in the brains of Huntington's disease patients. PMID: 25556834
  5. The findings of this study suggested that Rhes may play a crucial role in striatal iron homeostasis. PMID: 23999124
  6. Rhes robustly binds the autophagy regulator Beclin-1, decreasing its inhibitory interaction with Bcl-2 independent of JNK-1 signaling. PMID: 24324270
  7. The sequestering of Rhes through its binding to mutant huntingtin may decrease the ability of Rhes to perform vital physiological functions in the striatum neurons of Huntington disease patients. (Review) PMID: 23583659
  8. Rhes is an imidazoline-regulated transcript in pancreatic beta-cells PMID: 16945334
  9. Rhes can interfere with the functional activity of wt and mutated TSHr and with the respective hormone-stimulated cAMP production of FSHr and LHr. PMID: 17556863
  10. The genes RASD2 might be vulnerability genes for neuropsychologically defined subgroups of schizophrenic patients. PMID: 18571626
  11. Binding to the Gbeta subunits involves the cationic regions of AGS1 and Rhes, and Data used Rhes-AGS1 chimeras to show that their different cationic regions determine the Gbeta-specificity of the interactions. PMID: 19255495

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Subcellular Location
Cell membrane; Lipid-anchor.
Protein Families
Small GTPase superfamily, RasD family
Tissue Specificity
Pancreatic endocrine cells (islets of Langerhans).
Database Links

HGNC: 18229

OMIM: 612842

KEGG: hsa:23551

STRING: 9606.ENSP00000216127

UniGene: Hs.474711

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