Recombinant Human Gamma-crystallin C (CRYGC)

Code CSB-YP006019HU
MSDS
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Source Yeast
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Code CSB-EP006019HU
MSDS
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Source E.coli
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Code CSB-EP006019HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP006019HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP006019HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CRYGC
Uniprot No.
Alternative Names
CATARACT; VARIABLE ZONULAR PULVERULENT; CCL; CRGC_HUMAN; CRYG 3; Cryg 5; Cryg; CRYG3; CRYGC; Crystallin gamma 3; Crystallin gamma C; Gamma C crystallin; Gamma crystallin 2 1; Gamma crystallin 3; Gamma crystallin C; Gamma-C-crystallin; Gamma-crystallin 2-1; Gamma-crystallin 3; Gamma-crystallin C; Gammab cry; Len
Species
Homo sapiens (Human)
Expression Region
2-174
Target Protein Sequence
GKITFYEDR AFQGRSYETT TDCPNLQPYF SRCNSIRVES GCWMLYERPN YQGQQYLLRR GEYPDYQQWM GLSDSIRSCC LIPQTVSHRL RLYEREDHKG LMMELSEDCP SIQDRFHLSE IRSLHVLEGC WVLYELPNYR GRQYLLRPQE YRRCQDWGAM DAKAGSLRRV VDLY
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Crystallins are the dominant structural components of the vertebrate eye lens.
Gene References into Functions
  1. Study identified eight different mutations in CRYGC associated with autosomal dominant congenital nuclear cataracts (ADCC) in a cohort of Chinese family and shows that CRYGC mutations are responsible for 4.1% of ADCC families in the cohort. The results expand the spectrum of CRYGC mutations as well as their associated phenotypes. PMID: 28298635
  2. the G129C mutation in gammaC-crystallin, which is associated with autosomal dominant congenital nuclear cataract, perturbed the unfolding process by promoting the accumulation of two distinct aggregation-prone intermediates under mild denaturing conditions. PMID: 26165230
  3. Exome sequencing in developmental eye disease leads to identification of causal variants in GJA8, CRYGC, PAX6 and CYP1B1. PMID: 24281366
  4. We confirm that congenital cataract is associated with a CRYGC gene mutation. PMID: 23954869
  5. identified a CRYAA mutation in family A and a CRYGC mutation in family B with congenital cataract PMID: 23441109
  6. A nonsense mutation c.471G>A in CRYGC is associated with autosomal dominant congenital nuclear cataracts and microcornea in a Chinese pedigree. PMID: 22876111
  7. Molecular modeling and spectroscopic studies indicated that the mutation impaired the tertiary structure of gamma C crystallin by modifying the H-bonding network in the C-terminal domain. PMID: 22052681
  8. Transgenic expression of mutant CRYGC5bpd gamma-crystallin at near-physiological levels causes lens opacities and fiber cell defects, confirming the pathogenicity of this mutation. PMID: 21436266
  9. The T5P mutation obviously changes conformation and decreases conformational stability. PMID: 11904153
  10. the loss of interactions of T5P mutant of the gammaC-crystallin with other crystallins may play a larger role than the protection afforded by chaperone-like activity in Coppock-like cataract. PMID: 15322286
  11. This is the first case of phenotypic heterogeneity in the primary congenital cataract specifically associated with the R168W mutation in the CRYGC gene. PMID: 17679936
  12. Identification of a novel nonsense mutation in CRYGC in a Chinese family with autosomal dominant congenital nuclear cataracts and microcornea. PMID: 19204787

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Involvement in disease
Cataract 2, multiple types (CTRCT2)
Protein Families
Beta/gamma-crystallin family
Database Links

HGNC: 2410

OMIM: 123680

KEGG: hsa:1420

STRING: 9606.ENSP00000282141

UniGene: Hs.72910

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