Recombinant Human Glyceraldehyde-3-phosphate dehydrogenase, testis-specific (GAPDHS)

Code CSB-YP009254HU
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Source Yeast
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Code CSB-EP009254HU
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Source E.coli
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Code CSB-EP009254HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-MP009254HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GAPDHS
Uniprot No.
Alternative Names
G3PT_HUMAN; GAPD2; GAPDH 2 ; GAPDH-2; GAPDH2; GAPDHS; GAPDS; Glyceraldehyde 3 phosphate dehydrogenase spermatogenic; Glyceraldehyde 3 phosphate dehydrogenase; testis specific; Glyceraldehyde-3-phosphate dehydrogenase; HSD 35; HSD35; Spermatogenic cell specific glyceraldehyde 3 phosphate dehydrogenase 2; Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; Spermatogenic glyceraldehyde 3 phosphate dehydrogenase; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase; testis-specific
Species
Homo sapiens (Human)
Expression Region
1-408
Target Protein Sequence
MSKRDIVLTN VTVVQLLRQP CPVTRAPPPP EPKAEVEPQP QPEPTPVREE IKPPPPPLPP HPATPPPKMV SVARELTVGI NGFGRIGRLV LRACMEKGVK VVAVNDPFID PEYMVYMFKY DSTHGRYKGS VEFRNGQLVV DNHEISVYQC KEPKQIPWRA VGSPYVVEST GVYLSIQAAS DHISAGAQRV VISAPSPDAP MFVMGVNEND YNPGSMNIVS NASCTTNCLA PLAKVIHERF GIVEGLMTTV HSYTATQKTV DGPSRKAWRD GRGAHQNIIP ASTGAAKAVT KVIPELKGKL TGMAFRVPTP DVSVVDLTCR LAQPAPYSAI KEAVKAAAKG PMAGILAYTE DEVVSTDFLG DTHSSIFDAK AGIALNDNFV KLISWYDNEY GYSHRVVDLL RYMFSRDK
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
May play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. Required for sperm motility and male fertility.
Gene References into Functions
  1. GAPDS overexpression antagonize high glucose-induced apoptosis by controlling reactive oxygen species accumulation in TM3 cells. PMID: 29626473
  2. It is assumed that the deficiency in GAPDS observed in most dysplasia of the fibrous sheath sperm samples is ascribable to a disorder in the regulation of GAPDS expression caused by the mutation in the intron region of GAPDS gene. PMID: 27135296
  3. This result implies that GAPDHS participates in specific signal-transduction pathways. PMID: 26660717
  4. Structural basis for the NAD binding cooperativity and catalytic characteristics of sperm-specific glyceraldehyde-3-phosphate dehydrogenase PMID: 25936797
  5. GAPDS protein was expressed in both Sertoli cells and elongated sperms. The expression of GAPDS gradually increased with age in the epididymis. PMID: 23306140
  6. Data indicate that the positive expression of studied genes fertilization rate for GAPDHS positive subset was 66%, ACR - 71%, SPATA22 - 68%, MND1 - 70%, pregnancy rates were 8%, 6%, 18% and 36% respectively. PMID: 23675907
  7. the expression of GAPDS in melanoma cells may facilitate glycolysis and prevent the induction of apoptosis. PMID: 23026046
  8. The chaperonin TRiC was shown to assist an ATP-dependent refolding of recombinant forms of GAPDS. PMID: 21995946
  9. Structure and kinetic characterization of human sperm-specific glyceraldehyde-3-phosphate dehydrogenase, GAPDS. PMID: 21269272
  10. Human his-GAPDHS expressed in baculovirus-infected insect cells is homotetrameric. PMID: 20828617
  11. Data suggest that high stability of the sperm-specific GAPDS is of importance for the efficiency of fertilization. PMID: 20833277
  12. We have concluded that a simple nuclear localization of GAPDH does not induce apoptosis, and that MPP+-induced apoptosis is not caused by nuclear translocation of GAPDH. PMID: 16324157
  13. Comparative study of the amino acid sequences of mammalian GAPDs revealed conservative cysteine residues (C21, C94, and C150) that are specific for the sperm isoenzyme and absent in the somatic isoenzyme. PMID: 18298375
  14. Characterization of the rat GAPDHS and comparison to the human and mouse enzymes. PMID: 16700075
  15. GAPD2 enzymatic activity is present in human sperm. GAPD2 is tightly bound to sperm and most enzymatic activity is not released by permeabilization with CHAPS detergent. GAPD2 is restricted in location to the principal piece of the sperm flagellum. PMID: 10714828

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Subcellular Location
Cytoplasm.
Protein Families
Glyceraldehyde-3-phosphate dehydrogenase family
Tissue Specificity
Testis specific.
Database Links

HGNC: 24864

OMIM: 609169

KEGG: hsa:26330

STRING: 9606.ENSP00000222286

UniGene: Hs.248017

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