Recombinant Human Granzyme M (GZMM)

Code CSB-YP010085HU
MSDS
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Source Yeast
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Code CSB-EP010085HU
MSDS
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Source E.coli
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Code CSB-EP010085HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP010085HU
MSDS
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Source Baculovirus
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Code CSB-MP010085HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GZMM
Uniprot No.
Alternative Names
GRAM_HUMAN; Granzyme M (lymphocyte met ase 1); Granzyme M; GranzymeM; GZM M; Gzmm; HU Met 1; HU-Met-1; LMET 1; LMET1; Lymphocyte met ase 1; MET 1; Met 1 serine protease; Met ase; Met-1 serine protease; Met-ase; MET1; Met1 serine protease; Natural killer cell granular protease
Species
Homo sapiens (Human)
Expression Region
26-257
Target Protein Sequence
IIGGR EVIPHSRPYM ASLQRNGSHL CGGVLVHPKW VLTAAHCLAQ RMAQLRLVLG LHTLDSPGLT FHIKAAIQHP RYKPVPALEN DLALLQLDGK VKPSRTIRPL ALPSKRQVVA AGTRCSMAGW GLTHQGGRLS RVLRELDLQV LDTRMCNNSR FWNGSLSPSM VCLAADSKDQ APCKGDSGGP LVCGKGRVLA RVLSFSSRVC TDIFKPPVAT AVAPYVSWIR KVTGRSA
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Cleaves peptide substrates after methionine, leucine, and norleucine. Physiological substrates include EZR, alpha-tubulins and the apoptosis inhibitor BIRC5/Survivin. Promotes caspase activation and subsequent apoptosis of target cells.
Gene References into Functions
  1. GzMM has a critical role during early stages of inflammation in ulcerative colitis; in its absence colonic inflammation is enhanced. PMID: 27441655
  2. We also report that TNFalpha induces the attachment of Met1-linked Ub chains directly to TNF receptor 1, which do not seem to be attached covalently to Lys63-linked or other types of ubiquitin chain. PMID: 27133719
  3. Granzym M may be important in regulating HCMV latency and reactivation in SCT patients. PMID: 24316590
  4. GrM is expressed in HCMV-specific CD8thorn T cells and cleaves host cell protein hnRNP K in the presence of RNA. PMID: 23099853
  5. FADD cleavage by NK cell granzyme M enhances its self-association to facilitate procaspase-8 recruitment for auto-processing leading to caspase cascade. PMID: 21979465
  6. Results indicate that cellular overexpression of SERPINB4 inhibits recombinant GrM-induced as well as NK cell-mediated cell death. PMID: 21857942
  7. Results describe the structural characterization of granzyme M by molecular modeling as well as by detailed comparison with other granzymes. PMID: 20107908
  8. Noncytotoxic inhibition of cytomegalovirus replication through NK cell protease granzyme M-mediated cleavage of viral phosphoprotein 71. PMID: 21059895
  9. survivin cleavage by Granzyme M triggers degradation of the survivin-X-linked inhibitor of apoptosis protein (XIAP) complex to free caspase activity leading to cytolysis of target tumor cells PMID: 20406824
  10. These findings support not only a role for GrM in the innate but also in the adaptive immune response. PMID: 19896187
  11. GM expression is a distinctive feature of the nasal NK/T-cell, gamma delta T-cell, and intestinal T-cell lymphomas, and suggest that these tumors develop from lymphocytes involved in innate immunity. PMID: 12506019
  12. granzyme M represents a third major and specialized perforin-dependent cell death pathway that plays a significant role in death mediated by NK cells PMID: 15028722
  13. Granzyme (Gzm)M can directly degrade inhibitor of caspase-activated DNase (ICAD) to activate CAD, leading to DNA damage; GzmM cleaves DNA damage sensor enzyme poly(ADP-ribose) polymerase to prevent cellular DNA repair and force apoptosis. PMID: 16818775
  14. Granzyme M targets major components of the cytoskeleton that likely contribute to natural killer (NK) cell-induced cell death. PMID: 18523284
  15. NPM is essential for cell viability, these data suggest that targeting of NPM by granzyme M may contribute to tumor cell eradication by abolishing NPM function. PMID: 19103589
  16. Structure-based mutagenesis reveals that the amino-terminus and catalytic triad of GzmM are most essential for proteolytic function. PMID: 19542453

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Subcellular Location
Secreted. Cytoplasmic granule. Note=Granules of large granular lymphocytes.
Protein Families
Peptidase S1 family, Granzyme subfamily
Tissue Specificity
Highly and constitutively expressed in activated natural killer (NK) cells.
Database Links

HGNC: 4712

OMIM: 600311

KEGG: hsa:3004

STRING: 9606.ENSP00000264553

UniGene: Hs.465511

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