Recombinant Human Hepcidin(HAMP)

Code CSB-YP010124HU
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Source Yeast
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Code CSB-EP010124HU
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Source E.coli
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Code CSB-EP010124HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP010124HU
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Source Baculovirus
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Code CSB-MP010124HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names HAMP
Uniprot No. P81172
Alternative Names Hamp; HEPC; HEPC_HUMAN; Hepc20; Hepc25; HEPCIDIN ; Hepcidin 20; Hepcidin 25; Hepcidin antimicrobial peptide ; Hepcidin-20; Hepcidin25; HFE2; HFE2B; LEAP 1; LEAP-1; LEAP1; Liver expressed antimicrobial peptide; Liver-expressed antimicrobial peptide 1; PLTR; Putative liver tumor regressor
Species Homo sapiens (Human)
Expression Region 60-84
Target Protein Sequence D THFPICIFCC GCCHRSKCGM CCKT
Protein Length Cytoplasmic domain
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma
Gene References into Functions
  1. Hepcidin is lower in more severely anemic children with sickle cell disease independent of inflammation or markers of erythropoiesis. PMID: 30056060
  2. serum hepcidin level has a role in iron status in children with cystic fibrosis PMID: 30057485
  3. Athree-dimensional (3D) model of hepcidin-25 with bound copper(II) is presented. PMID: 30072660
  4. our data demonstrate that hypoxia strongly potentiates the peroxide-mediated induction of hepcidin via STAT3 signaling pathway. Moreover, oxidases such as NOX4 or artificially overexpressed urate oxidase (UOX) can induce hepcidin PMID: 29459227
  5. High hepcidin maternal serum levels could be an early marker of preeclampsia. PMID: 29523273
  6. Suggest IL-6 can induce hepcidin in systemic lupus erythematosus flares. PMID: 27416847
  7. Serum hepcidin was associated with more severe anemia in advanced CKD patients. PMID: 29227972
  8. Data suggest that magnitude of up-regulation of serum hepcidin levels in response to acute exercise in adults is dependent on pre-exercise iron nutritional status (ferritin levels) and on circulating pro-inflammatory cytokines (prominently interleukin-6). [REVIEW] PMID: 29443922
  9. The present study provides new evidence that ASP decreases hepcidin expression, which can reduce iron burden and inhibit tumor proliferation. These findings might aid ASP developed as a potential candidate for cancer treatment in patients with iron overload. PMID: 29843136
  10. Postexercise supplementation with protein and carbohydrate and vitamins D3 and K2 did not blunt the postexercise hepcidin response in highly trained athletes. PMID: 28605609
  11. A decrease in serum FGF23 and hepcidin levels was observed in chronic hemodialysis patients treated with lanthanum carbonate. PMID: 27928636
  12. These observations suggest correlations between serum hepcidin and progression of chronic HBV infection, and may shed a new light on the development of biomarkers for HBV-related disease surveillance. PMID: 27694815
  13. Increasing adiposity in the pediatric population is associated with increasing IL-6, which stimulates hepcidin synthesis, leading to functional iron deficiency due to inhibition of iron absorption and mobilization from iron stores. PMID: 28604755
  14. Suggest that pentoxifylline may be a clinically and biologically meaningful modulator of hepcidin-25 in dialysis of patients with ESA-hyporesponsive anaemia. PMID: 27171136
  15. Among low birth weight infants, gestational age, IL-6, erythropoietin, and soluble transferrin receptor were associated with Hep25 levels. Therefore, prematurity, inflammation, hypoxia, and erythropoietic activity may be important perinatal factors that affect hepcidin levels. PMID: 28601871
  16. genetic association studies in cohort of infants in Spain: Data suggest that serum hepcidin levels increase in infants during first year of life and are positively associated with iron status only in infants with wild-type HFE gene (not in infants with genetic polymorphisms C282Y, H63D, and S65C). (HFE, homeostatic iron regulator) PMID: 29404719
  17. Data suggest that circulating hepcidin levels (a biomarker for iron-deficiency anemia) may be regulated by dietary factors other than iron; here, one-time high-dose vitamin D3 reduces plasma hepcidin levels in adults one week post-dosing, without changes in plasma pro-inflammatory cytokine or ferritin concentrations. PMID: 27402475
  18. Hepcidin can very well be utilized as a potential prognostic marker to follow patients with breast cancer metastatic to bone. PMID: 28442512
  19. Data suggest that hematologic parameters in children consuming lacto-ovo vegetarian diets are comparable with those of control children, but ferritin levels are lower; inclusion of novel serum biomarkers, soluble transferrin receptor and hepcidin, in nutritional assessment can better detect subclinical iron deficiency in children following vegetarian diet. This study was conducted in Poland with children ages 4.5-9 years. PMID: 28342014
  20. results suggest that lower Hepcidin-25, as well as higher sTfR and sTfR/Hepcidin-25 ratio were significant predictors of favorable hemoglobin response within a month after IV administration of ferric carboxymaltose in patients with CKD PMID: 27282576
  21. unreported iron metabolism-related genes in non-classic hereditary hemochromatosis patients that were predicted to be potentially pathogenic were three novel mutations in TFR2 [two missense (p.Leu750Pro and p.Ala777Val) and one intronic splicing mutation (c.967-1G>C)], one missense mutation in HFE (p.Tyr230Cys), and one mutation in the 5'-UTR of HAMP gene (c.-25G>A) PMID: 27667161
  22. increased intracellular iron content in recombinant-TfR1 HepG2 cells did not increase hepcidin responses compared to wild-type cells, resembling hemochromatosis PMID: 27667164
  23. Review of the role of hepcidin in iron metabolism disorders. PMID: 28073521
  24. The bone morphogenetic protein (BMP) pathway regulates expression of hepcidin through transcriptional activation via BMP-responsive elements (REs) 1 and 2 on the promoter. A search for GC-rich sequences on the hepcidin promoter indicated 13 regions across the distal (A to F), middle (G to I), and proximal (J to M) areas. PMID: 29105755
  25. Of the non-HFE forms of iron overload, TFR2-, HFE2-, and HAMP-related forms are predicted to be rare, with pathogenic allele frequencies in the range of 0.00007 to 0.0005. Significantly, SLC40A1 variants that have been previously associated with autosomal-dominant ferroportin disease were identified in several populations (pathogenic allele frequency 0.0004), being most prevalent among Africans PMID: 26633544
  26. Data (including data from studies using knockout mice) suggest that MT2/TMPRSS6 suppresses hepcidin expression in hepatocytes independently of HJV; MT2/TMPRSS6 cleaves ALK2, ALK3, ACTRIIA, BMPR2, HFE, and, to a lesser extent, HJV and TFR2; thus, MT2/TMPRSS6 suppresses hepcidin expression by cleaving multiple components of the hepcidin induction pathway. (MT2/TMPRSS6 = matriptase-2; HJV = hemojuvelin) PMID: 28924039
  27. two unrelated hepatocellular carcinoma patients bore the HAMP:c.-153C>T mutation at the heterozygous state, which is associated with increased risk of iron overload and severe hemochromatosis PMID: 26474245
  28. Hepcidin-25 released from plaque macrophages and other cell surfaces contributed to the plaque instability by inducing endothelial cell death. PMID: 27855289
  29. Hepcidin plasma levels were increased in patients with early rheumatoid arthritis compared with healthy volunteers. PMID: 28482738
  30. study followed the dynamics of hepcidin-mediated ferroportin internalization; also showed that the novel p.D84E mutation, associated with the classical form of ferroportin disease, is both iron transport defective and hepcidin insensitive PMID: 28681497
  31. During regulation of hepcidin synthesis, multiple promoter elements in the HAMP gene respond to variable signaling pathways corresponding to different extracellular situations. PMID: 28501597
  32. Our findings indicate that the HAMP-P -582A>G polymorphism (rs10421768) is associated with susceptibility to extrapulmonary TB, but not pulmonary TB. CD14+ monocytes from individuals with the rs10421768 GG genotype secreted significantly less hepcidin in response to M. tuberculosis lipoarabinomannan compared with cells from individuals with either the AA or AG genotypes. PMID: 28530443
  33. Expression of Hepcidin and Ferroportin in the Placenta, and Ferritin and Transferrin Receptor 1 Levels in Maternal and Umbilical Cord Blood in Pregnant Women with and without Gestational Diabetes PMID: 27483296
  34. this study shows that hepcidin is involved in the pathogenesis of sepsis-induced acute kidney injury PMID: 27266727
  35. The hepcidin plays a role in the pathogenesis and progression of kidney injury via modulation of iron-mediated oxidant injury. PMID: 28302014
  36. The levels of hepcidin were higher, while those of iron, transferrin, and sTfR were lower in children with cardiometabolic risk factors. PMID: 27139516
  37. Main pathways of systemic and genetic regulation of hepcidin, as well as its influence on the disorders related to iron metabolism. Review. PMID: 28514781
  38. Data suggest hepcidin is the master regulator of systemic iron homeostasis; hepcidin levels are suppressed when erythropoiesis is stimulated; the erythroid-derived hormone erythroferrone appears to be a convincing candidate for link between increased erythropoiesis and hepcidin suppression. [REVIEW] PMID: 27146013
  39. Data suggest that proinflammatory cytokine interleukin-1beta (IL1B) up-regulates hepcidin expression in hepatocytes; inflammation induces IL1B production in Kupffer cells and hepatocytes; IL1B up-regulates CEBPD (CCAAT/enhancer binding protein delta) expression in hepatocytes; up-regulation of CEBPD expression up-regulates hepcidin transcription. PMID: 28438835
  40. modification of HS structure mediated by heparanase overexpression affects hepcidin expression and iron homeostasis PMID: 27711215
  41. Serum hepcidin levels increased in premenopausal women participating in brisk walking exercises. PMID: 28361576
  42. Gene-based meta-analyses revealed 19 genes that showed significant association with hepcidin. Our results suggest the absence of common SNVs and rare exonic SNVs explaining a large proportion of phenotypic variation in serum hepcidin PMID: 27846281
  43. hepcidin induction by endoplasmic reticulum stress involves the central SMAD1/5/8 pathway PMID: 27483343
  44. a selective splicing variant of hepcidin mRNA lacking exon 2 of HAMP gene, producing the transcript that encodes truncated peptide lacking 20 amino acids at the middle of preprohepcidin, was found in hepatocellular carcinoma cell lines PMID: 27264950
  45. Gastric H. pylori infection is a common cause of IDA of unknown origin in adult patients. Our results provide evidence indicating that hepcidin level decreases after successful H. pylori eradication with improvement in IDA. PMID: 26932797
  46. The data of in vitro and in vivo research evidenced on involvement of Hepc in formation of breast cancer cells malignant phenotype and their resistance to doxorubicin. PMID: 27356575
  47. Hepcidin was a more influential determinant of iron stores than blood loss and dietary factors combined, and increased hepcidin diminished the positive association between iron intake and iron stores. PMID: 27598194
  48. These results suggest that the ingestion of a high (compared to low) CHO diet over a seven-day training period is ineffective in attenuating post-exercise IL-6 and hepcidin responses. Such results may be due to the modest training load, the increased protein intake in the low-CHO trial, and a 48 h recovery period prior to sample collection on day 7, allowing a full recovery of muscle glycogen status between exercise sessi PMID: 27379793
  49. The NGAL/hepcidin ratio is more strongly associated with severe of acute kidney injury than the single biomarkers alone PMID: 25479470
  50. serum NGAL and hepcidin levels might be valuable for the evaluation of inflammation in chronic kidney disease, not related through iron metabolism PMID: 26627016
  51. study shows that patients with CRA had high expression of BMP6 and hepcidin and low expression of s-HJV. BMP6 was found to be negatively correlated with s-HJV; both regulate hepcidin expression and play important roles in the development of anemia. PMID: 27051019
  52. REVIEW: outline of recent discoveries in understanding how hepcidin and its receptor ferroportin are regulated, how they contribute to anemia of inflammation, and how this knowledge may help guide new diagnostic and therapeutic strategies for this disease PMID: 26886082
  53. During pregnancy, in women with sufficient iron supplementation, hepcidin is low and does not reflect iron status. During delivery and the postpartum period, hepcidin functions as a marker of inflammation. PMID: 26212583
  54. TGF-beta1 may contribute to hepcidin synthesis during iron overload. PMID: 27129231
  55. Low hepcidin levels are associated with type 2 diabetes mellitus. PMID: 26378394
  56. Hepcidin levels are higher in NAFLD with iron overload. PMID: 26405701
  57. Systemic inflammation and elevated values of IL-6 present in exacerbations and stabile chronic obstructive pulmonary disease might be responsible for the observed increased hepcidin level. PMID: 26164540
  58. The concentration of functional membrane-associated ferroportin is controlled by its ligand, the iron-regulatory hormone hepcidin, and fine-tuned by regulatory mechanisms serving iron homeostasis, oxygen utilization, host defense, and erythropoiesis. PMID: 26437604
  59. The serum hepcidin, ferritin, alanine transaminase, aspartate transaminase, gamma-glutamyltransferase and bilirubin were examined in two independent Chinese cohorts consisted of 3455 individuals. PMID: 26290281
  60. Hepcidin-25 shows a strong positive correlation with ferritin, and also correlates positively with CRP, in this patient population. PMID: 25598191
  61. the effect of alpha-1-antitrypsin (A1AT) on the expression of the iron hormone hepcidin PMID: 26310624
  62. Hepcidin levels are decreased independently from hs-CRP levels as a compensatory mechanism to increase the iron absorption in response to decreased serum iron levels in patients with RVD. PMID: 24569625
  63. erythropoietic drive, iron status, and inflammation all contribute to variation in hepcidin level. PMID: 26460251
  64. gonadotropin-stimulated endogenous estrogen markedly decreases circulating hepcidin-25 levels PMID: 26866603
  65. Identify 3 heterozygous missense mutations in BMP6 in patients with unexplained iron overload. These mutations lead to loss of signaling to SMAD proteins and reduced hepcidin production. PMID: 26582087
  66. Low hepcidin and high ferroportin expression by erythroblasts and macrophages were seen in iron deficiency anemia, while the opposite was true in anemia of chronic disorders. PMID: 25745821
  67. Ceramide induced the binding of STAT3, but not NF-kappaB or c-Jun, to HAMP promoter.The mutation of STAT3 response element within 0.6 kb HAMP promoter region significantly inhibited the stimulatory effect of ceramide on HAMP promoter activity. PMID: 26807955
  68. Mean prohepcidin levels were higher in premature newborns with oxygen radical diseases than in the control group. PMID: 25363011
  69. analysis of plasma hepcidin and anemia status in Zimbabwean infants, adjusted for sex, age and birthweight PMID: 26252205
  70. Parallel determination of liver hepcidin mRNA and serum hepcidin in patients with chronic liver diseases shows that circulating hepcidin and its respective ratio to ferritin are significantly diminished in patients with autoimmune liver diseases. PMID: 26270641
  71. Studies indicate plasma and urine hepcidin as a marker of anemia of chronic disease and post-transplant anemia (PTA). PMID: 26981017
  72. the effects of hepcidin expression and hepcidin peptide on brain iron contents, iron transport across the brain-blood barrier, iron uptake and release, and also the expression of TfR1, DMT1, and Fpn, were investigated. PMID: 25115800
  73. At univariate logistic regression analysis, high pulse wave velocity was associated with higher ferritin levels (p=0.010), but lower hepcidin (p=0.045), and hepcidin ferritin/ratio (p<0.001). PMID: 26244503
  74. TLR4 dependent macrophage signaling is controlled via hepcidin-ferroportin1 axis by influencing TLR4-lipid raft interactions PMID: 26125411
  75. Hepcidin level was significantly related to degree of anemia, indicating that an increase in hepcidin level will result in anemia. PMID: 25222239
  76. results clarify how commensal bacteria affect hepcidin expression and reveal a novel connection between IL-1beta and activation of BMP signaling. PMID: 26515063
  77. Studies suggest that hepcidin may become a useful tool for diagnosis and management of iron disorders. PMID: 25976966
  78. Abundant hepcidin protein found in brain parenchyma in the presence of levels of gene transcription that appear to be below the limits of detection is consistent with an origin for at least a proportion of hepcidin outside the brain PMID: 25896789
  79. Hepcidin levels are increased in acute ischemic stroke. PMID: 25881778
  80. Elevated second trimester serum hepcidin levels were associated with a higher risk of adverse pregnancy outcomes and high third trimester hepcidin levels were associated with higher risk of neonatal complications. PMID: 24946025
  81. Single nucleotide polymorphisms in HAMP, BMP2, FTL and SLC40A1 genes have phenotype-modifying roles in hereditary hemochromatosis type 1. PMID: 25976471
  82. hepcidin-25 was associated with erythropoietin and impaired kidney function in diabetic chronic kidney disease. PMID: 25894587
  83. lipids mediate post-transcriptional regulation of HAMP through PKC- and HuR-dependent mechanisms PMID: 26304124
  84. Decreased expression of hepcidin and ferroportin in hepatitis C patients indicates the importance of hepatocytic iron retention for viral response during pegylated-interferon plus ribavirin treatment. PMID: 25805936
  85. liver congestion induces hepcidin production, which may result in anemia and functional iron deficiency in some patients with heart failure. PMID: 25742771
  86. Elevated serum hepcidin levels are associated with the presence and severity of obstructive sleep apnea syndrome PMID: 25553801
  87. Inflammation regulates hepcidin transcription, allowing for sufficient concentrations of iron for organismal needs while sequestering the metal from infectious pathogens. [Review] PMID: 26055723
  88. hepcidin was significantly higher in heart allograft recipients compared with patients with chronic heart failure. PMID: 25380934
  89. Hepcidin is a promising serological marker for the differential diagnosis of tumor-related ACD and IDA, clarifying the pathogenesis of these anemias and guiding corrective treatment. PMID: 24954786
  90. Hereditary hypotransferrinemia caused by novel transferrin mutations can lead to elevated transferrin saturation and, when associated with HFE or HAMP mutations, to iron overload. PMID: 25486930
  91. Iron overload and increased serum levels of hepcidin plays an important role in the pathogenesis of endometriosis. PMID: 26137768
  92. These results establish a new connection between iron metabolism and prostate cancer, and suggest that prostatic dysregulation of hepcidin contributes to prostate cancer growth and progression. PMID: 25858146
  93. ferristatin II synergized with BMP6 and IL-6 to enhance hepcidin expression in vitro, However, this synergy was not due to activation of either Smad or Stat3 signaling PMID: 25907691
  94. significant difference in serum levels according to time of day PMID: 26118202
  95. hepcidin level is significantly increased in patients with chronic renal failure on maintenance hemodialysis. PMID: 25501198
  96. Data indicate that the hepcidin promoter bioassay system is useful for identifying and evaluating compounds that modulate hepcidin expression. PMID: 25633564
  97. The lower serum and saliva prohepcidin and hepcidin concentrations found in recurrent aphthous stomatitis and Behcet's disease patients indicate that hepcidin may be involved in the aetiopathogenesis of these diseases. PMID: 24341321
  98. knockdown of CD81 did not affect the stimulation of hepcidin expression by BMP6 but increased both the expression of ID1 and SMAD7, direct targets of BMP signaling pathway, and the phosphorylation of ERK1/2 PMID: 25635054
  99. Peripheral expression of hepcidin was measured using quantitative real time PCR in 50 beta-thalassemia major patients, in addition to 20 healthy volunteers as a control group. Hepcidin levels in beta-thalassemia major patients showed statistically significant decrease in comparison to the control group, and was correlated to cardiac iron stores. PMID: 25816754
  100. Chlorpyrifos inhibits hepatic hepcidin expression. PMID: 25176568

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Involvement in disease Hemochromatosis 2B (HFE2B)
Subcellular Location Secreted
Protein Families Hepcidin family
Tissue Specificity Highest expression in liver and to a lesser extent in heart and brain. Low levels in lung, tonsils, salivary gland, trachea, prostate gland, adrenal gland and thyroid gland. Secreted into the urine.
Database Links

HGNC: 15598

OMIM: 606464

KEGG: hsa:57817

STRING: 9606.ENSP00000222304

UniGene: Hs.8821

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