Recombinant Human Heterogeneous nuclear ribonucleoprotein F (HNRNPF)

Code CSB-YP010608HU
MSDS
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Source Yeast
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Code CSB-EP010608HU
MSDS
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Source E.coli
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Code CSB-EP010608HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP010608HU
MSDS
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Source Baculovirus
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Code CSB-MP010608HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
HNRNPF
Uniprot No.
Alternative Names
Heterogeneous nuclear ribonucleoprotein F; hnRNP F; HnRNP F protein; Hnrnpf; HNRPF; HNRPF_HUMAN; mcs94 1; MGC110997; N-terminally processed; Nucleolin like protein; Nucleolin like protein mcs94 1; Nucleolin-like protein mcs94-1; OK/SW-cl.23; OTTHUMP00000019482; OTTHUMP00000043413; OTTHUMP00000043414; Ribonucleoprotein F
Species
Homo sapiens (Human)
Expression Region
1-415
Target Protein Sequence
MMLGPEGGEG FVVKLRGLPW SCSVEDVQNF LSDCTIHDGA AGVHFIYTRE GRQSGEAFVE LGSEDDVKMA LKKDRESMGH RYIEVFKSHR TEMDWVLKHS GPNSADSAND GFVRLRGLPF GCTKEEIVQF FSGLEIVPNG ITLPVDPEGK ITGEAFVQFA SQELAEKALG KHKERIGHRY IEVFKSSQEE VRSYSDPPLK FMSVQRPGPY DRPGTARRYI GIVKQAGLER MRPGAYSTGY GGYEEYSGLS DGYGFTTDLF GRDLSYCLSG MYDHRYGDSE FTVQSTTGHC VHMRGLPYKA TENDIYNFFS PLNPVRVHIE IGPDGRVTGE ADVEFATHEE AVAAMSKDRA NMQHRYIELF LNSTTGASNG AYSSQVMQGM GVSAAQATYS GLESQSVSGC YGAGYSGQNS MGGYD
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state.
Gene References into Functions
  1. hnRNP H/F are important for maintenance and differentiation of embryonic stem cells and that this at least in part reflects a switch in TCF3 alternative splicing that leads to repression of CDH1/E-cadherin. PMID: 30115631
  2. Results from a study on gene expression variability markers in early-stage human embryos shows that HNRNPF is a putative marker for the 3-day, 8-cell embryo stage. PMID: 26288249
  3. heterogeneous nuclear ribonucleoprotein F (hnRNPF) regulates G-quadruplex-associated alternative splicing across the transcriptome. PMID: 29269483
  4. Simulation results demonstrate that the RNA binding domain qRRM2 of hnRNP F binds strongly with G-tract RNA, but any mutation of the G-tract leads to a drastic reduction of the binding free energy. PMID: 29050934
  5. Human T2D kidneys exhibited more RPTC apoptosis and lower expression of hnRNP F, SIRTUIN-1, and FOXO3alpha than nondiabetic kidneys. PMID: 28424160
  6. hnRNP F is a co-factor in a subset of tristetraprolin/BRF1/BRF2-mediated mRNA decay. PMID: 24978456
  7. this result suggests that hnRNP F directs formation of the exon 4 minus variant of ENOX2. PMID: 21625959
  8. The study shows solution structures of the three quasi-RNA-recognition motifs of heterogeneous nuclear ribonucleoprotein F in complex with G-tract RNA. PMID: 20526337
  9. heterogeneous ribonucleoprotein F is involved in the regulation of cell proliferation via the mammalian target of rapamycin/S6 kinase 2 pathway PMID: 20308064
  10. Results suggest that hnRNP H and F are nuclear shuttling proteins whose posttranslational modifications may alter interaction with transportin 1, nuclear localization, and hence function. PMID: 20308327
  11. high levels of hnRNP F are present in premalignant and malignant stages of colorectal cancer, reflecting a role for this protein early in colorectal tumorigenesis PMID: 16424007
  12. The solution structure of the three quasi RNA recognition motifs of hnRNP F and identification of the residues that are important for the interaction with the Bcl-x RNA. PMID: 16885237
  13. hnRNPH and F regulate DM20 splicing by recruiting U1snRNP and hnRNPH plays a primary role in DM20 splice site selection in vivo PMID: 19244236
  14. Shows that hnRNPF regulates the 3' end cleavage reaction. PMID: 11158309

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Subcellular Location
Nucleus, nucleoplasm.
Tissue Specificity
Expressed ubiquitously.
Database Links

HGNC: 5039

OMIM: 601037

KEGG: hsa:3185

STRING: 9606.ENSP00000338477

UniGene: Hs.712955

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