Recombinant Human High mobility group nucleosome-binding domain-containing protein 5 (HMGN5)

Code CSB-YP016099HU
MSDS
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Source Yeast
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Code CSB-EP016099HU
MSDS
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Source E.coli
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Code CSB-EP016099HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP016099HU
MSDS
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Source Baculovirus
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Code CSB-MP016099HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
HMGN5
Uniprot No.
Alternative Names
High mobility group nucleosome binding domain 5; High mobility group nucleosome-binding domain-containing protein 5; Hmgn5; HMGN5_HUMAN; NBP 45; NSBP 1; NSBP1; Nucleosomal binding protein 1; Nucleosome binding protein 1; Nucleosome-binding protein 1
Species
Homo sapiens (Human)
Expression Region
1-282
Target Protein Sequence
MPKRKAAGQG DMRQEPKRRS ARLSAMLVPV TPEVKPKRTS SSRKMKTKSD MMEENIDTSA QAVAETKQEA VVEEDYNENA KNGEAKITEA PASEKEIVEV KEENIEDATE KGGEKKEAVA AEVKNEEEDQ KEDEEDQNEE KGEAGKEDKD EKGEEDGKED KNGNEKGEDA KEKEDGKKGE DGKGNGEDGK EKGEDEKEEE DRKETGDGKE NEDGKEKGDK KEGKDVKVKE DEKEREDGKE DEGGNEEEAG KEKEDLKEEE EGKEEDEIKE DDGKKEEPQS IV
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Preferentially binds to euchromatin and modulates cellular transcription by counteracting linker histone-mediated chromatin compaction.
Gene References into Functions
  1. Findings suggest that a miR-140-5p/HMGN5/autophagy regulatory loop plays a critical role in chemoresistance in osteosarcoma. In conclusion, our data elucidated that miR-140-5p promoted autophagy mediated by HMGN5 and sensitized osteosarcoma cells to chemotherapy. PMID: 28341864
  2. High HMGN5 expression is associated with osteosarcoma invasion. PMID: 28627703
  3. We observed decreased miR-409-3p expression in glioma, and overexpression of miR-409-3p repressed glioma cell invasion and proliferation by targeting HMGN5, an oncogene associated with glioma PMID: 28109076
  4. HMGN5 regulated the expression of multidrug resistance 1, cyclin B1, and Bcl-2. PMID: 28914995
  5. NSBP1 was highly expressed in NSCLC cells. NSBP1 siRNA knockdown suppressed NSCLC cell proliferation and invasion. miR326 has a putative binding site in the NSBP1 3'UTR. NSBP1 overexpression abolished miR326 inhibition of cyclin B1 and MMP9 expression. PMID: 26548724
  6. HMGN5 overexpression is correlated with advanced pathological grade and poorer prognosis in meningiomas PMID: 26315299
  7. Decreased miR-340 expression may contribute to the development and progression of prostate cancer through a mechanism that involves HMGN5. PMID: 26394192
  8. Our data first time identified miR-186 as the upstream regulator of NSBP1 PMID: 26290438
  9. High HMGN5 expression is associated with urothelial bladder cancer. PMID: 25796505
  10. HMGN5 is an oncogene and plays an important role in prostate cancer tumorigenesis and progression. the level of HMGN5 may be used as a biomarker to predict the patients that would benefit from gemcitabine treatment. PMID: 25572120
  11. HMGN5 plays an oncogenic role in human breast cancer by inhibiting cell proliferation and invasion, and activating apoptosis, which could be exploited as a target for therapy in human breast cancer. PMID: 25315189
  12. HMGN5 knockdown sensitizes prostate cancer cells to ionizing radiation PMID: 25307178
  13. HMGN5 plays oncogenic role in osteosarcoma by promoting cell proliferation and invasion, and could be exploited as a target for therapy in osteosarcoma. PMID: 24687550
  14. HMGN5 is a critical factor in the development of chemoresistance through regulating autophagy, and it offers a novel target for improving osteosarcoma therapy. PMID: 24664583
  15. Data indicate that cells lacking either high mobility group protein N5 (HMGN5) and lamina-associated polypeptide 2alpha (LAP2alpha) showed that loss of either protein affects the genome-wide distribution of the remaining partner. PMID: 23673662
  16. HMGN5 silencing to significantly inhibit A549 and H1299 cell proliferation. PMID: 22994738
  17. HMGN5 may be a potential molecular target with therapeutic relevance for the treatment of prostate cancer. PMID: 22504871
  18. NSBP1 plays oncogenic role in clear cell renal cell carcinoma (ccRCC) by promoting cell proliferation and invasion. PMID: 22420896
  19. The data of study suggested that HMGN5 may play a critical role in the development of gliomas. PMID: 21373965
  20. these results suggest that NSBP1 promotes the viability of bladder cancer cells through increased cell proliferation PMID: 21695596
  21. HMGN5 has a highly disordered structure, binds dynamically to nucleosome core particles, modulates the binding of H1 to chromatin, reduces the compaction of the chromatin fiber, and affects transcription. PMID: 21518955
  22. A positive correlation was observed between NSBP1 expression and cell proliferation and apoptosis in DU145 cells. PMID: 20531280
  23. The structure of the HMGN5 gene and the known properties of the HMGN5 protein, are described. PMID: 20123071

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Subcellular Location
Nucleus.
Protein Families
HMGN family
Tissue Specificity
Ubiquitously expressed.
Database Links

HGNC: 8013

OMIM: 300385

KEGG: hsa:79366

STRING: 9606.ENSP00000350848

UniGene: Hs.282204

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