Recombinant Human Histone H1.5 (H1-5)

Code CSB-YP010377HU
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP010377HU
MSDS
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Source E.coli
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Code CSB-EP010377HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP010377HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP010377HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
H1-5
Uniprot No.
Alternative Names
H1; H1 histone family member 5; H1.5; H15 HUMAN; H15_HUMAN; H1B; H1F5; H1s 3; Hist1h1b; Histone 1 H1b; Histone cluster 1 H1b; Histone H1.5; Histone H1a; Histone H1b; Histone H1s 3; MGC126630; MGC126632
Species
Homo sapiens (Human)
Expression Region
2-226
Target Protein Sequence
SETAPAETA TPAPVEKSPA KKKATKKAAG AGAAKRKATG PPVSELITKA VAASKERNGL SLAALKKALA AGGYDVEKNN SRIKLGLKSL VSKGTLVQTK GTGASGSFKL NKKAASGEAK PKAKKAGAAK AKKPAGATPK KAKKAAGAKK AVKKTPKKAK KPAAAGVKKV AKSPKKAKAA AKPKKATKSP AKPKAVKPKA AKPKAAKPKA AKPKAAKAKK AAAKKK
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation.
Gene References into Functions
  1. Data indicate that normal ovarian tissues strongly expressed histone H1.5, whereas ovarian granulosa cell tumors (GCTs) weakly expressed this protein; in contrast, PLZF protein expression was not significantly different between both study groups. PMID: 25023763
  2. Statistically significant differences in staining patterns were found for histone H1.5, distinguishing leiomyosarcomas from leiomyomas. PMID: 24784718
  3. Promyelocytic leukemia zinc finger and histone H1.5 differentially stain low- and high-grade pulmonary neuroendocrine tumors PMID: 23416030
  4. FoxP3 interacts with H1.5 to alter its binding to target genes to modulate their expression and to program Treg function. PMID: 21654845
  5. the mode of the chromatin fiber compaction changes depending both on salt environment and linker histone H1 PMID: 16185066
  6. Phosphorylation of human H1 variants occurs nonrandomly during both interphase and mitosis and distinct serine- or threonine-specific kinases are involved in different cell cycle phases. PMID: 16377619
  7. Phosphorylation at threonine 10 appears in prometaphase and disappears in telophase, and that this hyperphosphorylated form of H1.5 is mainly chromatin-bound in metaphase when chromatin condensation is maximal. PMID: 19136008

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Subcellular Location
Nucleus. Chromosome. Note=According to PubMed:15911621 more commonly found in heterochromatin. According to PubMed:10997781 associates with actively transcribed chromatin and not heterochromatin.
Protein Families
Histone H1/H5 family
Tissue Specificity
Ubiquitous. Expressed in the majority of the cell lines tested and in testis.
Database Links

HGNC: 4719

OMIM: 142711

KEGG: hsa:3009

STRING: 9606.ENSP00000330074

UniGene: Hs.131956

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