Recombinant Human Homeodomain-interacting protein kinase 4 (HIPK4)

Code CSB-YP854106HU
MSDS
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Source Yeast
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Code CSB-EP854106HU
MSDS
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Source E.coli
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Code CSB-EP854106HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP854106HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP854106HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
HIPK4
Uniprot No.
Alternative Names
HIPK 4; HIPK4; HIPK4_HUMAN; Homeodomain interacting protein kinase 4; Homeodomain-interacting protein kinase 4
Species
Homo sapiens (Human)
Expression Region
1-616
Target Protein Sequence
MSTIQSETDC YDIIEVLGKG TFGEVAKGWR RSTGEMVAIK ILKNDAYRNR IIKNELKLLH CMRGLDPEEA HVIRFLEFFH DALKFYLVFE LLEQNLFEFQ KENNFAPLPA RHIRTVTLQV LTALARLKEL AIIHADLKPE NIMLVDQTRC PFRVKVIDFG SASIFSEVRY VKEPYIQSRF YRAPEILLGL PFCEKVDVWS LGCVMAELHL GWPLYPGNNE YDQVRYICET QGLPKPHLLH AACKAHHFFK RNPHPDAANP WQLKSSADYL AETKVRPLER RKYMLKSLDQ IETVNGGSVA SRLTFPDREA LAEHADLKSM VELIKRMLTW ESHERISPSA ALRHPFVSMQ QLRSAHETTH YYQLSLRSYR LSLQVEGKPP TPVVAAEDGT PYYCLAEEKE AAGMGSVAGS SPFFREEKAP GMQRAIDQLD DLSLQEAGHG LWGETCTNAV SDMMVPLKAA ITGHHVPDSG PEPILAFYSS RLAGRHKARK PPAGSKSDSN FSNLIRLSQV SPEDDRPCRG SSWEEGEHLG ASAEPLAILQ RDEDGPNIDN MTMEAERPDP ELFDPSSCPG EWLSEPDCTL ESVRGPRAQG LPPRRSHQHG PPRGATSFLQ HVTGHH
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Protein kinase that phosphorylates human TP53 at Ser-9, and thus induces TP53 repression of BIRC5 promoter. May act as a corepressor of transcription factors (Potential).
Gene References into Functions
  1. loss of HIPK4 expression improves early skin epithelial differentiation accompanied by an increase in K8 and K18 expression, and improves late keratinocyte differentiation with an increase of epidermal identity PMID: 28966120
  2. Analysis of these mutants revealed that HIPK1, HIPK2 and HIPK3 but not HIPK4 are capable of autophosphorylating on other tyrosines PMID: 25630557
  3. homeodomain interacting protein kinase 4 could phosphorylate human p53 protein at serine 9, both in vitro and in vivo PMID: 18022393
Subcellular Location
Cytoplasm.
Protein Families
Protein kinase superfamily, CMGC Ser/Thr protein kinase family, HIPK subfamily
Database Links

HGNC: 19007

OMIM: 611712

KEGG: hsa:147746

STRING: 9606.ENSP00000291823

UniGene: Hs.79363

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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