Recombinant Human Interferon regulatory factor 2 (IRF2)

Code CSB-YP011815HU
MSDS
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Source Yeast
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Code CSB-EP011815HU
MSDS
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Source E.coli
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Code CSB-EP011815HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP011815HU
MSDS
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Source Baculovirus
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Code CSB-MP011815HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
IRF2
Uniprot No.
Alternative Names
DKFZp686F0244; Interferon regulatory factor 2; IRF 2; IRF-2; IRF2; IRF2_HUMAN
Species
Homo sapiens (Human)
Expression Region
1-349
Target Protein Sequence
MPVERMRMRP WLEEQINSNT IPGLKWLNKE KKIFQIPWMH AARHGWDVEK DAPLFRNWAI HTGKHQPGVD KPDPKTWKAN FRCAMNSLPD IEEVKDKSIK KGNNAFRVYR MLPLSERPSK KGKKPKTEKE DKVKHIKQEP VESSLGLSNG VSDLSPEYAV LTSTIKNEVD STVNIIVVGQ SHLDSNIENQ EIVTNPPDIC QVVEVTTESD EQPVSMSELY PLQISPVSSY AESETTDSVP SDEESAEGRP HWRKRNIEGK QYLSNMGTRG SYLLPGMASF VTSNKPDLQV TIKEESNPVP YNSSWPPFQD LPLSSSMTPA SSSSRPDRET RASVIKKTSD ITQARVKSC
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and represses those genes. Also acts as an activator for several genes including H4 and IL7. Constitutively binds to the ISRE promoter to activate IL7. Involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. Antagonizes IRF1 transcriptional activation.
Gene References into Functions
  1. Data suggest that MIR1290 expression is remarkably upregulated in non-small-cell lung carcinoma tissues compared to adjacent normal lung tissues; IRF2 appears to be a direct target of MIR1290. (MIR1290 = microRNA-1290; IRF2 = interferon regulatory factor-2) PMID: 29275213
  2. These finding suggests that miR-302b inhibits key transcription factors and cytokines by targeting ERBB4, IRF2 and CXCR4, implicating its role in the inhibition of CRI in EC. PMID: 28467773
  3. Our study primarily suggests IRF-2 as a potential prognostic biomarker in colorectal cancer PMID: 28465494
  4. The effects of IRF2 suppresses non-small cell lung cancer by promoting cell apoptosis, inhibiting cell proliferation and migration ability. PMID: 28471447
  5. In a genome wide are study to identify acute kidney injury risk in critically ill patients, the locus on chromosome 4, located 150 kb upstream of IRF2 was identified to regulate immunity pathways related to kidney disease risk gene APOL1. Disruption of IRF-2 has been found to up-regulate the inflammatory response to infection. PMID: 27576016
  6. Study shows that IRF2 knockdown inhibits growth, colony formation of OCI/AML-2, OCI/AML-3, and THP-1 cells. In addition, IRF2 knockdown induces apoptosis of acute myeloid leukemia (AML) cells by regulating apoptotic effectors. Further mechanism analysis shows that INPP4B contributes to the effects of IRF2 on apoptosis and growth of AML cells. Thus, IRF2 serves as an important regulator in AML by targeting INPP4B. PMID: 28579269
  7. miR-450 targets IRF2 and thus supresses lung cancer cell proliferation and invasion. PMID: 27246609
  8. It might play as a tumor suppressor by regulating P53 signaling in gastric cancer. PMID: 26173586
  9. the association of IRF2 with susceptibility to systemic lupus erythematosus PMID: 25285625
  10. IRF2genes were not associated with pancreatitis. 4 variants were found: c.123G>A (novel), c.651C>T, c.744G>A, and c.638C>T, p.P213L. PMID: 25207663
  11. miR-221 directly inhibits the expression of SOCS3 and IRF2. PMID: 24607843
  12. IFN regulatory factor 2 (Irf2) has a regulatory role in trypsinogen5 gene transcription, which is resistant to a major endogenous trypsin inhibitor, Spink3 PMID: 22042864
  13. IRF2 overexpression protects against I/R injury by decreasing IRF1-dependent injury and may represent a novel therapeutic strategy. PMID: 22744333
  14. The results suggested that distinct markers in IRF2 may be associated with atopic dermatitis and eczema herpeticum (which may depend upon ethnic ancestry) and genetic variants in IRF2 may contribute to an abnormal immune response to herpes simplex virus. PMID: 22113474
  15. these results suggest that IRF-2 plays an important role in the tumorigenesis of pancreatic cancer and down-regulation of IRF-2 would be a new treatment target for pancreatic cancer. PMID: 22119988
  16. IRF-2 activates the HPV-16 P97 promoter in vivo. PMID: 20129639
  17. IRF-2 activates RIG-I promoter through ISRE-like site as well as IRF-1 and IFN-alpha stimulation. PMID: 20034464
  18. PTB binding to multiple sites within IRF2 5'UTR leads to a conformational change in the RNA that facilitate binding of other trans-acting factors to mediate internal initiation of translation PMID: 19756143
  19. IRF2 is a potential susceptibility gene for psoriasis. PMID: 14962090
  20. IRF-2 is involved in up-regulation of nonmuscle myosin heavy chain II-A gene expression in cell differentiation PMID: 15496418
  21. In cells transfected with both IRF-2 and p300/CBP-associated factor , IRF-2 associated with endogenous nucleolin. PMID: 16582966
  22. IRF8 cooperates with PU.1 and IRF-2 to activate a composite ets/IRF-cis element in the NF1 promoter. The conserved IRF domain tyrosine in ICSBP/IRF8 is required for interaction with the DNA-bound PU.1-IRF2 heterodimer. PMID: 17200120
  23. IRF-2 regulates NF-kappaB activity via the modulation of NF-kappaB subcellular localization. PMID: 18395009
  24. IRF2 interacts with the SUMO-E3 ligase PIASy and is sumoylated in vivo PMID: 18514056

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Subcellular Location
Nucleus.
Protein Families
IRF family
Tissue Specificity
Expressed throughout the epithelium of the colon. Also expressed in lamina propria.
Database Links

HGNC: 6117

OMIM: 147576

KEGG: hsa:3660

STRING: 9606.ENSP00000377218

UniGene: Hs.654566

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