Recombinant Human Iroquois-class homeodomain protein IRX-3 (IRX3)

Code CSB-YP011835HU
MSDS
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Source Yeast
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Code CSB-EP011835HU
MSDS
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Source E.coli
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Code CSB-EP011835HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP011835HU
MSDS
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Source Baculovirus
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Code CSB-MP011835HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
IRX3
Uniprot No.
Alternative Names
AI894186; Homeodomain protein IRXB1; Iroquois class homeodomain protein IRX 1; Iroquois homeobox 3; Iroquois homeobox protein 3; Iroquois-class homeodomain protein irx-3; IRX 1; irx3; IRX3_HUMAN; IRXB1
Species
Homo sapiens (Human)
Expression Region
1-501
Target Protein Sequence
MSFPQLGYQY IRPLYPSERP GAAGGSGGSA GARGGLGAGA SELNASGSLS NVLSSVYGAP YAAAAAAAAA QGYGAFLPYA AELPIFPQLG AQYELKDSPG VQHPAAAAAF PHPHPAFYPY GQYQFGDPSR PKNATRESTS TLKAWLNEHR KNPYPTKGEK IMLAIITKMT LTQVSTWFAN ARRRLKKENK MTWAPRSRTD EEGNAYGSER EEEDEEEDEE DGKRELELEE EELGGEEEDT GGEGLADDDE DEEIDLENLD GAATEPELSL AGAARRDGDL GLGPISDSKN SDSEDSSEGL EDRPLPVLSL APAPPPVAVA SPSLPSPPVS LDPCAPAPAP ASALQKPKIW SLAETATSPD NPRRSPPGAG GSPPGAAVAP SALQLSPAAA AAAAHRLVSA PLGKFPAWTN RPFPGPPPGP RLHPLSLLGS APPHLLGLPG AAGHPAAAAA FARPAEPEGG TDRCSALEVE KKLLKTAFQP VPRRPQNHLD AALVLSALSS S
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcription factor involved in SHH-dependent neural patterning. Together with NKX2-2 and NKX6-1 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class I proteins of neuronal progenitor factors, which are repressed by SHH signals. Involved in the transcriptional repression of MNX1 in non-motor neuron cells. Acts as a regulator of energy metabolism.
Gene References into Functions
  1. IRX3 genetic variants associate with birth weight, body mass index and AST/ALT-related transaminase metabolism, supporting the role of IRX3 as an obesity-associated susceptibility gene. PMID: 28316138
  2. Results show that IRX3 deficiency repressed the browning program of white adipocytes partially by regulating UCP1 transcriptional activity. Rare variants of IRX3 were associated with human obesity. PMID: 28988979
  3. Increased adipocyte-specific expression of IRX3 correlates with the presence of the FTO obesity risk haplotype in lean children, whereas it was unaffected by risk variants in obese peers. IRX3 expression in adipocytes was significantly related to adipocyte hypertrophy. PMID: 27560134
  4. Downregulation of miR-377 may contribute to the upregulation of IRX3 in HCC. PMID: 27222047
  5. Strategies to pharmacologically regulate Irx3 function in adult endothelial cells may provide new therapies for angiogenesis. PMID: 25512384
  6. IRX3 function may have a direct functional relationship to both obesity and type 2 diabetes. PMID: 20080751

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Subcellular Location
Nucleus.
Protein Families
TALE/IRO homeobox family
Database Links

HGNC: 14360

OMIM: 612985

KEGG: hsa:79191

STRING: 9606.ENSP00000331608

UniGene: Hs.499205

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