Recombinant Human Kelch-like protein 24 (KLHL24)

Code CSB-YP750981HU
MSDS
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Source Yeast
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Code CSB-EP750981HU
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Source E.coli
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Code CSB-EP750981HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP750981HU
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Source Baculovirus
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Code CSB-MP750981HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
KLHL24
Uniprot No.
Alternative Names
KLHL24; DRE1; Kelch-like protein 24; Kainate receptor-interacting protein for GluR6; KRIP6; Protein DRE1
Species
Homo sapiens (Human)
Expression Region
1-600
Target Protein Sequence
MVLILGRRLN REDLGVRDSP ATKRKVFEMD PKSLTGHEFF DFSSGSSHAE NILQIFNEFR DSRLFTDVII CVEGKEFPCH RAVLSACSSY FRAMFCNDHR ESREMLVEIN GILAEAMECF LQYVYTGKVK ITTENVQYLF ETSSLFQISV LRDACAKFLE EQLDPCNCLG IQRFADTHSL KTLFTKCKNF ALQTFEDVSQ HEEFLELDKD ELIDYICSDE LVIGKEEMVF EAVMRWVYRA VDLRRPLLHE LLTHVRLPLL HPNYFVQTVE VDQLIQNSPE CYQLLHEARR YHILGNEMMS PRTRPRRSTG YSEVIVVVGG CERVGGFNLP YTECYDPVTG EWKSLAKLPE FTKSEYAVCA LRNDILVSGG RINSRDVWIY NSQLNIWIRV ASLNKGRWRH KMAVLLGKVY VVGGYDGQNR LSSVECYDSF SNRWTEVAPL KEAVSSPAVT SCVGKLFVIG GGPDDNTCSD KVQSYDPETN SWLLRAAIPI AKRCITAVSL NNLIYVAGGL TKAIYCYDPV EDYWMHVQNT FSRQENCGMS VCNGKIYILG GRRENGEATD TILCYDPATS IITGVAAMPR PVSYHGCVTI HRYNEKCFKL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Necessary to maintain the balance between intermediate filament stability and degradation, a process that is essential for skin integrity. As part of the BCR(KLHL24) E3 ubiquitin ligase complex, mediates ubiquitination of KRT14 and controls its levels during keratinocytes differentiation. Specifically reduces kainate receptor-mediated currents in hippocampal neurons, most probably by modulating channel properties.
Gene References into Functions
  1. KLHL24 mutation is associated in the pathogenesis of skin abnormalities and epidermolysis bullosa simplex. PMID: 28532758
  2. KLHL24 Mutation is associated with epidermolysis bullosa. PMID: 27798626
  3. findings identify KLHL24 mutations as a cause of skin fragility and identify a role for KLHL24 in maintaining the balance between intermediate filament stability and degradation required for skin integrity PMID: 27889062
  4. Data show that a miRNA, hsv1-mir-H27, encoded within the genome of herpes simplex virus 1 (HSV-1), targets the mRNA of the cellular transcriptional repressor Kelch-like 24 (KLHL24). PMID: 23512275
  5. KRIP6 regulates kainate receptors by inhibiting PICK1 modulation via competition or a mutual blocking effect PMID: 18692513

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Involvement in disease
Epidermolysis bullosa simplex, generalized, with scarring and hair loss (EBSSH)
Subcellular Location
Perikaryon. Cell projection, axon. Cytoplasm. Cell junction, desmosome. Cell junction, adherens junction.
Tissue Specificity
Expressed in the skin. Found in keratinocytes, dermal fibroblasts, and melanocytes. Basal-layer keratinocytes have lower KLHL24 expression than suprabasal keratinocytes.
Database Links

HGNC: 25947

OMIM: 611295

KEGG: hsa:54800

STRING: 9606.ENSP00000242810

UniGene: Hs.407709

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