Recombinant Human Large subunit GTPase 1 homolog (LSG1)

Code CSB-YP861981HU
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Source Yeast
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Code CSB-EP861981HU
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Source E.coli
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Code CSB-EP861981HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP861981HU
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Source Baculovirus
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Code CSB-MP861981HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
LSG1
Uniprot No.
Alternative Names
LSG1; Large subunit GTPase 1 homolog; hLsg1; EC 3.6.1.-
Species
Homo sapiens (Human)
Expression Region
1-658
Target Protein Sequence
MGRRRAPAGG SLGRALMRHQ TQRSRSHRHT DSWLHTSELN DGYDWGRLNL QSVTEQSSLD DFLATAELAG TEFVAEKLNI KFVPAEARTG LLSFEESQRI KKLHEENKQF LCIPRRPNWN QNTTPEELKQ AEKDNFLEWR RQLVRLEEEQ KLILTPFERN LDFWRQLWRV IERSDIVVQI VDARNPLLFR CEDLECYVKE MDANKENVIL INKADLLTAE QRSAWAMYFE KEDVKVIFWS ALAGAIPLNG DSEEEANRDD RQSNTTKFGH SSFDQAEISH SESEHLPARD SPSLSENPTT DEDDSEYEDC PEEEEDDWQT CSEEDGPKEE DCSQDWKESS TADSEARSRK TPQKRQIHNF SHLVSKQELL ELFKELHTGR KVKDGQLTVG LVGYPNVGKS STINTIMGNK KVSVSATPGH TKHFQTLYVE PGLCLCDCPG LVMPSFVSTK AEMTCSGILP IDQMRDHVPP VSLVCQNIPR HVLEATYGIN IITPREDEDP HRPPTSEELL TAYGYMRGFM TAHGQPDQPR SARYILKDYV SGKLLYCHPP PGRDPVTFQH QHQRLLENKM NSDEIKMQLG RNKKAKQIEN IVDKTFFHQE NVRALTKGVQ AVMGYKPGSG VVTASTASSE NGAGKPWKKH GNRNKKEKSR RLYKHLDM
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
GTPase required for the XPO1/CRM1-mediated nuclear export of the 60S ribosomal subunit. Probably acts by mediating the release of NMD3 from the 60S ribosomal subunit after export into the cytoplasm (Probable).
Gene References into Functions
  1. hLsg1 is an essential GTPase predominantly located in the endoplasmic reticulum and, in some cells, in Cajal bodies in the nucleus. PMID: 16209721
Subcellular Location
Cytoplasm. Endoplasmic reticulum. Nucleus, Cajal body. Note=Shuttles between the Cajal bodies in the nucleus and the endoplasmic reticulum.
Protein Families
TRAFAC class YlqF/YawG GTPase family, LSG1 subfamily
Database Links

HGNC: 25652

OMIM: 610780

KEGG: hsa:55341

STRING: 9606.ENSP00000265245

UniGene: Hs.744061

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