Recombinant Human Lymphoid-specific helicase (HELLS), partial

Code CSB-YP868307HU
MSDS
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Source Yeast
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Code CSB-EP868307HU
MSDS
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Source E.coli
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Code CSB-EP868307HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP868307HU
MSDS
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Source Baculovirus
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Code CSB-MP868307HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
HELLS
Uniprot No.
Alternative Names
FLJ10339; Helicase lymphoid specific; Hells; HELLS_HUMAN; LSH; Lymphoid specific helicase; Lymphoid-specific helicase; Nbla10143; PASG; Proliferation associated SNF2 like protein; Proliferation-associated SNF2-like protein; SWI/SNF2 related matrix associated actin dependent regulator of chromatin subfamily A member 6; SWI/SNF2-related matrix-associated actin-dependent regulator of chromatin subfamily A member 6
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Plays an essential role in normal development and survival. Involved in regulation of the expansion or survival of lymphoid cells. Required for de novo or maintenance DNA methylation. May control silencing of the imprinted CDKN1C gene through DNA methylation. May play a role in formation and organization of heterochromatin, implying a functional role in the regulation of transcription and mitosis.
Gene References into Functions
  1. HELLS and CDCA7 comprise a bipartite nucleosome remodeling complex; immunodeficiency-centromeric instability-facial anomalies syndrome has a defective HELLS and CDCA7 bipartite nucleosome remodeling complex PMID: 29339483
  2. This study elucidates the molecular basis of the c-Myc/EGLN1-mediated induction of LSH expression that inhibits ferroptosis PMID: 28900510
  3. LSH is likely one of the mechanisms of genome instability underlying 5-hydroxymethylcytosine loss in cancer. PMID: 29109788
  4. High mRNA levels of HELLS is independent predictor of poor outcome in Renal Cell Carcinoma patients. PMID: 28069330
  5. Mutations in HELLS cause immunodeficiency-centromeric instability-facial anomalies syndrome type 4. PMID: 26216346
  6. Mutations of HELLS gene is associated with stomach and colorectal cancers. PMID: 25351940
  7. Upregulation of HELLS and UHRF1 is essential for the tumor phenotype. Also, these epigenetic regulators are important for the regulation of SYK. PMID: 25338120
  8. just as E2F3, HELLS is overexpressed in human tumours including prostate cancer, indicating that either factor may contribute to the malignant progression of tumours PMID: 22157815
  9. E2F1 plays a crucial role in transcriptional control of the human Lsh gene and the decrease of Lsh expression in senescent cells is related to the repression of E2F1 PMID: 21453717
  10. Data provide strong evidence that CEP55 and HELLS may be used in conjunction with FOXM1 as a biomarker set for early cancer detection and indicators of malignant conversion and progression. PMID: 20400365
  11. LSH serves as a recruiting factor for DNA methyltransferases and histone deacetylases to establish transcriptionally repressive chromatin which is perhaps further stabilized by DNA methylation at targeted loci PMID: 17967891
  12. These results suggest differences in cellular consequences of hypomethylation mediated by PASG during development compared to that in somatic cells. PMID: 18948754
  13. Lsh overexpression delays cell senescence by silencing p16(INK4a) in human fibroblasts. PMID: 19561196

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Involvement in disease
Immunodeficiency-centromeric instability-facial anomalies syndrome 4 (ICF4)
Subcellular Location
Nucleus.
Protein Families
SNF2/RAD54 helicase family
Tissue Specificity
Highly expressed in proliferative tissues such as adult thymus and testis, and expressed at lower levels in uterus, small intestine, colon, and peripheral blood mononuclear cells. Also expressed in neoplastic cell lines including those derived from myeloi
Database Links

HGNC: 4861

OMIM: 603946

KEGG: hsa:3070

STRING: 9606.ENSP00000239027

UniGene: Hs.463677

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