Recombinant Human Methionine aminopeptidase 1D, mitochondrial (METAP1D)

Code CSB-YP747607HU
MSDS
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Source Yeast
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Code CSB-EP747607HU
MSDS
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Source E.coli
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Code CSB-EP747607HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP747607HU
MSDS
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Source Baculovirus
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Code CSB-MP747607HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
METAP1D
Uniprot No.
Alternative Names
METAP1D; MAP1DMethionine aminopeptidase 1D; mitochondrial; MAP 1D; MetAP 1D; EC 3.4.11.18; Methionyl aminopeptidase type 1D; mitochondrial; Peptidase M 1D
Species
Homo sapiens (Human)
Expression Region
20-335
Target Protein Sequence
S SPLNHIYLHK QSSSQQRRNF FFRRQRDISH SIVLPAAVSS AHPVPKHIKK PDYVTTGIVP DWGDSIEVKN EDQIQGLHQA CQLARHVLLL AGKSLKVDMT TEEIDALVHR EIISHNAYPS PLGYGGFPKS VCTSVNNVLC HGIPDSRPLQ DGDIINIDVT VYYNGYHGDT SETFLVGNVD ECGKKLVEVA RRCRDEAIAA CRAGAPFSVI GNTISHITHQ NGFQVCPHFV GHGIGSYFHG HPEIWHHAND SDLPMEEGMA FTIEPIITEG SPEFKVLEDA WTVVSLDNQR SAQFEHTVLI TSRGAQILTK LPHEA
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed. May play a role in colon tumorigenesis.
Gene References into Functions
  1. Observational study of gene-disease association. (HuGE Navigator) PMID: 20877624
  2. Observational study of gene-disease association. (HuGE Navigator) PMID: 19913121
  3. Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator) PMID: 20628086
  4. Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20379614
  5. MAP1D is a potentially oncogenic, novel member of the MAP gene family that may play an important role in colon tumorigenesis PMID: 16568094

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Subcellular Location
Mitochondrion.
Protein Families
Peptidase M24A family, Methionine aminopeptidase type 1 subfamily
Tissue Specificity
Overexpressed in colon cancer cell lines and colon tumors as compared to normal tissues (at protein level).
Database Links

HGNC: 32583

OMIM: 610267

KEGG: hsa:254042

STRING: 9606.ENSP00000315152

UniGene: Hs.298250

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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