Name: Recombinant Human Methionine aminopeptidase 2 (METAP2)
Brand: CUSABIO
SKU: 013716HU

Product Details

Purity

>85% (SDS-PAGE)

Target Names

METAP2

Uniprot No.

P50579

Research Area

Metabolism

Alternative Names

A930035J23Rik; AI047573; AL024412; Amp2; AU014659,; EIF 2 associated p67 homolog; EIF-2-associated p67; Initiation factor 2 associated 67 kDa glycoprotein; Initiation factor 2-associated 67 kDa glycoprotein; MAP 2; MAP2; MAP2_HUMAN; MetAP 2; Metap2; Methionine aminopeptidase 2; Methionyl aminopeptidase 2; MGC102452; MGC127390; MGC53792; MNPEP; p67; p67eIF2; Peptidase M; Peptidase M2

Species

Homo sapiens (Human)

Expression Region

2-478aa

Target Protein Sequence

AGVEEVAASGSHLNGDLDPDDREEGAASTAEEAAKKKRRKKKKSKGPSAAGEQEPDKESGASVDEVARQLERSALEDKERDEDDEDGDGDGDGATGKKKKKKKKKRGPKVQTDPPSVPICDLYPNGVFPKGQECEYPPTQDGRTAAWRTTSEEKKALDQASEEIWNDFREAAEAHRQVRKYVMSWIKPGMTMIEICEKLEDCSRKLIKENGLNAGLAFPTGCSLNNCAAHYTPNAGDTTVLQYDDICKIDFGTHISGRIIDCAFTVTFNPKYDTLLKAVKDATNTGIKCAGIDVRLCDVGEAIQEVMESYEVEIDGKTYQVKPIRNLNGHSIGQYRIHAGKTVPIVKGGEATRMEEGEVYAIETFGSTGKGVVHDDMECSHYMKNFDVGHVPIRLPRTKHLLNVINENFGTLAFCRRWLDRLGESKYLMALKNLCDLGIVDPYPPLCDIKGSYTAQFEHTILLRPTCKEVVSRGDDY

Mol. Weight

54.7kD

Protein Length

Full Length of Mature Protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

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■ Q&A

Please may you assist me with a few questions regarding product CSB-BP013716HU?
1. Please could you confirm what the purity is? We noticed two figures, 85% and 90% so any clarification is appreciated!
2. Do you test for activity or expect this protein could be active?
3. Do you perform any validation?

Thanks for your inquiry.
Answers to your questions:
1.We noticed two figures, 85% and 90% so any clarification is appreciated! Purity of this protein is >85%.
2. We haven't tested the activity of this protein, cannot 100% guaranteed. What we provide is the full length of mature protein, and it is purified under mild condition. It is active in theory. Here is a reference reported the activity of protein derived from Baculovirus. Link: https://www.rndsystems.com/cn/products/recombinant-human-methionine-aminopeptidase-2-protein-cf_3795-zn
3. No, we haven't performed validation. We could perform if you need. Pls let us know.

Target Background

Function

Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo.; Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis.

Gene References into Functions

MetAP1 and MetAP2 have roles in driving cell selectivity for a potent anti-cancer agent in synergy, by controlling glutathione redox state PMID: 27542228
Suggest MetAP2 as important regulator of proliferation/apoptosis in non-small cell lung cancers. PMID: 26935506
Nt-acetylation prevents the excision of the initiator methionine by MetAP2. PMID: 25886145
Data indicate that methionine aminopeptidase 2 (MetAP2) contains a single disulfide bond that exists in oxidized and reduced states and influences enzyme function. PMID: 24700462
The activity of unmodified, nitrated and oxidised METAP2 was assessed and it was found that nitration significantly reduced its ability to cleave a chromogenic substrate. Mass spectrometry analysis identified Tyr336 as a nitrated residue in METAP2. PMID: 24041691
Increased expression of METAP2 is associated with pilocytic astrocytoma. PMID: 23161775
This study demonstrated a hitherto-undescribed role of MetAP2 in definitive hematopoiesis and a possible link to noncanonical Wnt and ERK signaling. PMID: 21937698
the substrate specificities of Escherichia coli MetAP1, human MetAP1, and human MetAP2 were systematically profiled PMID: 20521764
Data indicate that MetAP2 expression and pp60c-src phosphorylation were decreased in NC2213 treated cells. PMID: 19703310
the functional role of S100A4 in regulating endothelial cell growth and tumor metastasis involves interaction with the N-terminal half of Methionine Aminopeptidase 2. PMID: 11994292
High expression in germinal center B cells and their neoplastic counterparts. PMID: 12118091
Divalent metal cofactors are physiologically relevant for activity of this enzyme. PMID: 12718546
MetAP protein has a role in colorectal adenocarcinoma progression PMID: 15102683
Expressed in colon cancer cells PMID: 15336565
MetAP-1 and MetAP-2 have essential functions in the control of mammalian cell proliferation PMID: 15962312
A comparison of the structual differences between Type I and Type II methionine aminopeptidases. PMID: 16274222
Data suggest that methionine aminopeptidase 2 plays a role in the proliferation of fibroblasts and myofibroblasts in fibrotic lung diseases and may serve as a novel pharmacologic target in idiopathic pulmonary fibrosis. PMID: 17446530
MetAP2 plays an important role in tumor cell growth and may contribute to tumorigenesis PMID: 18264137
Discovery, identification, and characterization of candidate pharmacodynamic markers of METAP2 inhibition are reported. PMID: 18828628
Using bovine cell extract, fumagillin was found to be bound by methionine aminopeptidase (MetAP-2). PMID: 9177176

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Subcellular Location

Cytoplasm. Note=About 30% of expressed METAP2 associates with polysomes.

Protein Families

Peptidase M24A family, Methionine aminopeptidase eukaryotic type 2 subfamily

Database Links

HGNC: 16672

OMIM: 601870

KEGG: hsa:10988

STRING: 9606.ENSP00000325312

UniGene: Hs.444986

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Code	CSB-BP013716HU
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-EP013716HU-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-MP013716HU
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Human Methionine aminopeptidase 2 (METAP2)

Product Details

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Target Background

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