Recombinant Human Multivesicular body subunit 12B (FAM125B)

Code CSB-YP867167HU
MSDS
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Source Yeast
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Code CSB-EP867167HU
MSDS
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Source E.coli
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Code CSB-EP867167HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP867167HU
MSDS
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Source Baculovirus
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Code CSB-MP867167HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MVB12B
Uniprot No.
Alternative Names
MVB12B; C9orf28; FAM125B; Multivesicular body subunit 12B; ESCRT-I complex subunit MVB12B; Protein FAM125B
Species
Homo sapiens (Human)
Expression Region
1-319
Target Protein Sequence
MRSCFCVRRS RDPPPPQPPP PPPQRGTDQS TMPEVKDLSE ALPETSMDPI TGVGVVASRN RAPTGYDVVA QTADGVDADL WKDGLFKSKV TRYLCFTRSF SKENSHLGNV LVDMKLIDIK DTLPVGFIPI QETVDTQEVA FRKKRLCIKF IPRDSTEAAI CDIRIMGRTK QAPPQYTFIG ELNSMGIWYR MGRVPRNHDS SQPTTPSQSS AASTPAPNLP RHISLTLPAT FRGRNSTRTD YEYQHSNLYA ISAMDGVPFM ISEKFSCVPE SMQPFDLLGI TIKSLAEIEK EYEYSFRTEQ SAAARLPPSP TRCQQIPQS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies.
Gene References into Functions
  1. Study suggests a novel association between SNPs in FAM125B andintra-ocular pressure in the TwinsUK cohort. PMID: 24518671
  2. The 1.3-A atomic resolution crystal structure of the MVB12B MABP domain reveals a beta-prism fold, a hydrophobic membrane-anchoring loop, and an electropositive phosphoinositide-binding patch. PMID: 22232651
  3. These results suggest that the expression of MVB12B may be normally suppressed through the ubiquitin-proteasome pathway that simultaneously regulates the fate of MVB12A and the functions of ESCRT-I. PMID: 20654576
Subcellular Location
Endosome. Late endosome membrane; Peripheral membrane protein.
Protein Families
MVB12 family
Database Links

HGNC: 23368

KEGG: hsa:89853

STRING: 9606.ENSP00000354772

UniGene: Hs.162659

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