Recombinant Human Myosin light chain 3 (MYL3)

Code CSB-YP015310HU
MSDS
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Source Yeast
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Code CSB-EP015310HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP015310HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP015310HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MYL3
Uniprot No.
Alternative Names
Cardiac myosin light chain 1; CMH8 ; CMLC1; ELC of myosin; Essential light chain of myosin; MLC1SB; MLC1V; MYL3; MYL3_HUMAN; Myosin light chain 1; Myosin light chain 1 slow twitch muscle B/ventricular isoform; Myosin light chain 1 slow, B; Myosin light chain 3; Myosin, light chain 1, ventricular; myosin, light chain 3, alkali, ventricular, skeletal, slow; myosin, light polypeptide 3, alkali; myosin, light polypeptide 3, alkali, ventricular, skeletal, slow; OTTHUMP00000165922; Slow skeletal ventricular myosin alkali light chain 3; slow-twitch muscle B/ventricular isoform; Ventricular/slow twitch myosin alkali light chain; VLC1
Species
Homo sapiens (Human)
Expression Region
1-195
Target Protein Sequence
MAPKKPEPKK DDAKAAPKAA PAPAPPPEPE RPKEVEFDAS KIKIEFTPEQ IEEFKEAFML FDRTPKCEMK ITYGQCGDVL RALGQNPTQA EVLRVLGKPR QEELNTKMMD FETFLPMLQH ISKNKDTGTY EDFVEGLRVF DKEGNGTVMG AELRHVLATL GERLTEDEVE KLMAGQEDSN GCINYEAFVK HIMSS
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Regulatory light chain of myosin. Does not bind calcium.
Gene References into Functions
  1. In Familial Hypertrophic Cardiomyopathy, the MYL3 Arg94His variant was associated with high disease penetrance and substantial interventricular septal hypertrophy PMID: 26443374
  2. N-fragment is the binding domain of human ventricular LC1, whereas the C-fragment serves as a functional domain, which may be more involved in the modulation of the actin-activated ATPase activity of myosin PMID: 14516912
  3. Cell-permeable peptide containing the 15 amino acid N-terminal peptide from human ventricular light chain-1 (VLC-1) enhanced myocardial contractility. PMID: 17717642
Involvement in disease
Cardiomyopathy, familial hypertrophic 8 (CMH8)
Database Links

HGNC: 7584

OMIM: 160790

KEGG: hsa:4634

STRING: 9606.ENSP00000292327

UniGene: Hs.517939

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