Recombinant Human Negative elongation factor B (COBRA1)

Code CSB-YP845164HU
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Source Yeast
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Code CSB-EP845164HU
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Source E.coli
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Code CSB-EP845164HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP845164HU
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Source Baculovirus
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Code CSB-MP845164HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NELFB
Uniprot No.
Alternative Names
NELFB; COBRA1; KIAA1182; Negative elongation factor B; NELF-B; Cofactor of BRCA1
Species
Homo sapiens (Human)
Expression Region
1-580
Target Protein Sequence
MFAGLQDLGV ANGEDLKETL TNCTEPLKAI EQFQTENGVL LPSLQSALPF LDLHGTPRLE FHQSVFDELR DKLLERVSAI ASEGKAEERY KKLEDLLEKS FSLVKMPSLQ PVVMCVMKHL PKVPEKKLKL VMADKELYRA CAVEVKRQIW QDNQALFGDE VSPLLKQYIL EKESALFSTE LSVLHNFFSP SPKTRRQGEV VQRLTRMVGK NVKLYDMVLQ FLRTLFLRTR NVHYCTLRAE LLMSLHDLDV GEICTVDPCH KFTWCLDACI RERFVDSKRA RELQGFLDGV KKGQEQVLGD LSMILCDPFA INTLALSTVR HLQELVGQET LPRDSPDLLL LLRLLALGQG AWDMIDSQVF KEPKMEVELI TRFLPMLMSF LVDDYTFNVD QKLPAEEKAP VSYPNTLPES FTKFLQEQRM ACEVGLYYVL HITKQRNKNA LLRLLPGLVE TFGDLAFGDI FLHLLTGNLA LLADEFALED FCSSLFDGFF LTASPRKENV HRHALRLLIH LHPRVAPSKL EALQKALEPT GQSGEAVKEL YSQLGEKLEQ LDHRKPSPAQ AAETPALELP LPSVPAPAPL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. May be able to induce chromatin unfolding. Essential for early embryogenesis; plays an important role in maintaining the undifferentiated state of embryonic stem cells (ESCs) by preventing unscheduled expression of developmental genes. Plays a key role in establishing the responsiveness of stem cells to developmental cues; facilitates plasticity and cell fate commitment in ESCs by establishing the appropriate expression level of signaling molecules. Supports the transcription of genes involved in energy metabolism in cardiomyocytes; facilitates the association of transcription initiation factors with the promoters of the metabolism-related genes.; (Microbial infection) The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. In vitro, binds weakly to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1.
Gene References into Functions
  1. BRCA1 Interacting Protein COBRA1 Facilitates Adaptation to Castrate-Resistant Growth Conditions. PMID: 30036938
  2. A positively charged face of NELF-AC is involved in RNA binding, whereas the opposite face of the NELF-AC subcomplex binds NELF-B. NELF-B is predicted to form a HEAT repeat fold, also binds RNA in vivo, and anchors the subunit NELF-E, which is confirmed to bind RNA in vivo. PMID: 27282391
  3. High COBRA1 expression is associated with hepatocellular carcinoma. PMID: 28112367
  4. both human and mouse NELF-B proteins are translated from a non-AUG codon upstream of the annotated AUG. PMID: 26010750
  5. These studies allow us to position the actions of two new modulators of GR-regulated transactivation, NELF-A and NELF-B, relative to other factors in the overall gene induction sequence. PMID: 24097989
  6. data show that NELF subunits exhibit highly specific subcellular localizations, such as in NELF bodies or in midbodies, and some shuttle actively between the nucleus and cytoplasm; loss of NELF from cells can lead to enlarged and/or multiple nuclei PMID: 19245807
  7. NELF-C and NELF-D are highly related or identical to the protein called TH1, of unknown function. NELF-B and NELF-C or NELF-D are integral subunits that bring NELF-A and NELF-E together. [NELF-B] [NELF-C] PMID: 12612062
  8. differentially expressed in breast cancer cell lines, antibody to it may be a useful tool to investigate its functions PMID: 15185750
  9. COBRA1 may directly modulate AP-1 pathway and, therefore, may play important roles in cell proliferation, differentiation, apoptosis, and oncogenesis. PMID: 15530430
  10. COBRA1 can negatively regulate the activator protein-1 (AP-1) complex at the TFF1 promoter and thus down-regulate TFF1 expression in gastric cancer cell lines. PMID: 16452188
  11. Decreasing NELF also correlated with displacement of a positioned nucleosome and increased acetylation of histone H4, suggesting coupling of transcription elongation and chromatin remodeling PMID: 17442680
  12. Negative elongation factor (NELF) is a four subunit transcription factor. Our results point to a surprising role of NELF in the 3' end processing of histone mRNAs and suggest that NELF is a new factor that coordinates mRNA processing in transcription. PMID: 17499042
  13. COBRA1 may coordinate multiple steps in ligand-dependent gene expression, which in turn ensures both the quantity and quality of hormone-stimulated gene products PMID: 17659869
  14. a lack of COBRA1 expression in breast carcinoma may serve as a useful indicator for poor prognosis. PMID: 17910036
  15. COBRA1 and BRCA1 may engage in common gene regulatory pathways to suppress breast cancer progression. PMID: 18071589

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Subcellular Location
Nucleus.
Protein Families
NELF-B family
Tissue Specificity
Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.
Database Links

HGNC: 24324

OMIM: 611180

KEGG: hsa:25920

STRING: 9606.ENSP00000339495

UniGene: Hs.521809

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