Recombinant Human Neuroligin-4, X-linked (NLGN4X), partial

Code CSB-YP843131HU
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Source Yeast
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Code CSB-EP843131HU
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Source E.coli
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Code CSB-EP843131HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP843131HU
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Source Baculovirus
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Code CSB-MP843131HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NLGN4X
Uniprot No.
Alternative Names
ASPGX2; AUTSX2; HLNX; HNL4X; HNLX; KIAA1260; neuroligin 4, X-linked; Neuroligin X; Neuroligin-4; NL4; NLGN; NLGN4; NLGN4X; NLGNX_HUMAN; OTTHUMP00000022863; OTTHUMP00000022864; OTTHUMP00000022865; X-linked
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Putative neuronal cell surface protein involved in cell-cell-interactions.
Gene References into Functions
  1. NLGN4X might represent novel biomarkers and therapeutic targets for breast cancer. Inhibition of NLGN4X may be a new target for the prevention and treatment of breast cancer PMID: 29244827
  2. Noncoding polymorphisms on NLGN4X may be associated to autism suggesting the key role of NLGN4X in autism pathophysiology and in its male prevalence. PMID: 27782075
  3. Endogenous NLGN4X is intensely phosphorylated on T707 upon PKC stimulation in human neurons. PMID: 25675530
  4. In vitro models show NLGN4X knockdown directly impacts neurodevelopmental process during the formation of neurons and their connections. PMID: 23710042
  5. Five genes have been directly disrupted in Tourette Syndrome by independent genomic rearrangements and copy number variations with unique breakpoints. PMID: 22948383
  6. The autism-associated DeltaE4 mutation in NLGN4 compromises the ability of NLGN4 to localize correctly to the cell surface when overexpressed and to induce synaptic differentiation. PMID: 21278334
  7. results indicate that the genetic variants located in NLGN4 can affect the cognitive abilities of boys. PMID: 20714171
  8. Results suggest that unique conformational reshaping of the neuroligin 4 surface is required to permit neurexin 1beta association. PMID: 20543817
  9. finding further contributes to consideration of neuroligins as probable candidate genes for future molecular genetic studies, suggesting that a defect of synaptogenesis may predispose to autism PMID: 19645625
  10. Scanning and sequencing of 2.5Mb of the NLGN4 genes reveals an association of NLGN4 structural variants at highly conserved amino acids with an estimated attributable risk for autism of about 3% in the cohorts studies. PMID: 15622415
  11. Data indicate that coding mutations in neuroligin 4 are very rarely associated to autism spectrum disorders. PMID: 16508939
  12. Splice variants of the NLGN4 gene are associated with autism. PMID: 16648374
  13. Syntrophin-gamma2 (SNTG2) is a de novo binding partner of X-linked neuroligin 4, which correlates with autism-related mutations. PMID: 17292328
  14. NLGN4 mutations can be associated with a wide spectrum of neuropsychiatric conditions and that carriers may be affected with milder symptoms PMID: 18231125
  15. The results suggest a positive association between the genetic variants of the NLGN4 and NSMR in the Chinese children from Qinba Mountains Region. PMID: 19125102
  16. This study indicated that the phenotypic spectrum of NLGN4X mutations and overexpressed NLGN4X transcript is associated with autism and nonsyndromic mental profound mental retardation. PMID: 19545860
  17. NLGN4X gene is associated with autistic traits, empathy, and Asperger syndrome. PMID: 19598235
  18. Two brothers with classical autism spectrum disorder carry a single amino-acid substitution in neuroligin 4 (Arg87Trp). The substitution is absent from the brothers' asymptomatic parents, suggesting that it arose in the maternal germ line. PMID: 19726642

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Involvement in disease
Autism, X-linked 2 (AUTSX2); Asperger syndrome, X-linked, 2 (ASPGX2)
Subcellular Location
Cell membrane; Single-pass type I membrane protein. Cell junction, synapse, postsynaptic density membrane.
Protein Families
Type-B carboxylesterase/lipase family
Tissue Specificity
Expressed at highest levels in heart. Expressed at lower levels in liver, skeletal muscle and pancreas and at very low levels in brain.
Database Links

HGNC: 14287

OMIM: 300427

KEGG: hsa:57502

STRING: 9606.ENSP00000275857

UniGene: Hs.21107

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