Recombinant Human Nucleobindin-2(NUCB2)

Code CSB-YP016146HU
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Source Yeast
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Code CSB-EP016146HU
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Source E.coli
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Code CSB-EP016146HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP016146HU
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Source Baculovirus
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Code CSB-MP016146HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names NUCB2
Uniprot No. P80303
Alternative Names DNA binding protein NEFA; DNA-binding protein NEFA; Gastric cancer antigen Zg4; HEL S 109; NEFA; Nefastin 1; Nefastin-1; Nefastin1; Nucb2; NUCB2_HUMAN; Nucleobindin 2; Nucleobindin-2; Nucleobinding 2; Prepronefastin; Prepronesfatin
Species Homo sapiens (Human)
Expression Region 25-420
Target Protein Sequence VPIDID KTKVQNIHPV ESAKIEPPDT GLYYDEYLKQ VIDVLETDKH FREKLQKADI EEIKSGRLSK ELDLVSHHVR TKLDELKRQE VGRLRMLIKA KLDSLQDIGM DHQALLKQFD HLNHLNPDKF ESTDLDMLIK AATSDLEHYD KTRHEEFKKY EMMKEHERRE YLKTLNEEKR KEEESKFEEM KKKHENHPKV NHPGSKDQLK EVWEETDGLD PNDFDPKTFF KLHDVNSDGF LDEQELEALF TKELEKVYDP KNEEDDMVEM EEERLRMREH VMSEVDTNKD RLVTLEEFLK ATEKKEFLEP DSWETLDQQQ FFTEEELKEY ENIIALQENE LKKKADELQK QKEELQRQHD QLEAQKLEYH QVIQQMEQKK LQQGIPPSGP AGELKFEPHI
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Calcium-binding protein. May have a role in calcium homeostasis.; FUNCTION
Gene References into Functions
  1. Study data identified nesfatin-1 as a key modulator in hypertension and vascular remodeling by facilitating vascular smooth muscle cells phenotypic switching and proliferation. PMID: 29627363
  2. intensity of exercise may have an important role in changes of nesfatin-1, leptin, FFA, and epinephrine concentrations even though this was not the case for IL-6 and insulin resistance PMID: 25637303
  3. Patients with schizophrenia had significantly higher serum nesfatin-1 levels compared to healthy controls. PMID: 29278806
  4. This study finds that soccer matches performed different workout times have strong stimulatory effects on irisin levels in all subjects but nesfatin-1 response varied among the subjects and it did not change significantly in afternoon match. PMID: 30084805
  5. serum nesfatin-1 concentrations were inversely correlated with atrial fibrillation development. PMID: 29747779
  6. In all trimesters of human pregnancy, NUCB2/nesfatin-1 was highly expressed in syncytiotrophoblast. In addition, there was a significant increase in NUCB2 expression in human primary trophoblast cells induced to syncytialise. PMID: 28855042
  7. Data suggest that circulating nesfatin-1 levels are up-regulated in patients newly diagnosed with type 2 diabetes; non-newly diagnosed patients treated with antidiabetic medications exhibit lower circulating nesfatin-1 levels. [META-ANALYSIS, REVIEW] PMID: 29445751
  8. Data suggest that levels of nesfatin-1 in maternal serum and cord blood are up-regulated in women with gestational diabetes (GD) as compared to control pregnant women; nesfatin-1 also appears to be up-regulated in subcutaneous abdominal adipose tissue in GD. This study was conducted in China in women of the Han ethnicity. PMID: 28361552
  9. expressions of p-Akt/Akt and p-GSK-3beta/GSK-3beta in the myocardium of MI group rats were significantly increased by nesfatin-1 administration, suggesting that nesfatin-1, which appears to possess anti-apoptotic and anti-inflammatory properties, may confer protection against ISO-induced MI via an Akt/GSK-3beta-dependent mechanism. PMID: 28720397
  10. Study demonstrated that high expression level of NUCB2 was significantly associated with poor clinical outcome of patient with non-metastatic clear cell renal cell carcinoma. PMID: 27806328
  11. The multifunctional biological actions of nesfatin-1 propelled this peptide as a therapeutic target, and as a potential biomarker of diseases. However, a better and comprehensive understanding of tissue specific effects of nesfatin-1 is critical prior to exploring its possible use in the detection and treatment of diseases. PMID: 28137218
  12. Data indicate that nesfatin-1/NUCB-2 enhanced migration, invasion and epithelial-mesenchymal transition (EMT) in colon cancer cells through LKB1/AMPK/TORC1/ZEB1 pathways in vitro and in vivo. PMID: 27150059
  13. Nesfatin-1 levels decrease following biliopancreatic diversion with duodenal switch-induced weight loss and are significantly associated with parameters of metabolic health. PMID: 27681383
  14. women and men showed an inverse association between NUCB2/nesfatin-1 and anxiety with a positive correlation in women and a negative correlation in men. However, no significant change of NUCB2/nesfatin-1 following improvement of anxiety has been observed. PMID: 28273586
  15. Our study was the first to investigate the nesfatin 1 levels in patients with the first episode psychosis. Based on our study results, nesfatin 1 might be related to some central nervous system pathologies, including the severity of a psychiatric disorder. PMID: 28814086
  16. nucleobindin 2 might play a crucial role in gastric cancer development and could serve as an independent predictor of prognosis of gastric cancer patients. PMID: 28714371
  17. Based on the obtained results, it was proposed that ghrelin may be considered as playing a role in the etiopathogenesis of hyperemesis gravidarum that may result in disruption of the relationship between nesfatin-1 and ghrelin. PMID: 27235705
  18. This study supports the role of insulin resistance in decreased nesfatin-1 levels in patients with Type 2 diabetes mellitus and metabolic syndrome PMID: 27539099
  19. Increased plasma nesfatin-1 concentrations are associated closely with inflammation, trauma severity and clinical outcomes, indicating that nesfatin-1 might be involved in inflammation and become a good prognostic biomarker following traumatic brain injury PMID: 27155585
  20. Data suggest that NUCB2 (almost negligible in normal endometrium) plays pivotal roles in cell proliferation and migration/invasiveness of endometrial carcinoma; NUCB2 may serve as a prognostic biomarker for endometrial carcinoma. Expression of NUCB2 appears almost negligible in normal endometrium. PMID: 26842712
  21. we summarize nesfatin-1 main functions, focusing on its cardiovascular implications--{REVIEW} PMID: 26662184
  22. NUCB2 protein levels in the obese PCOS group were significantly lower than those in the non-obese PCOS and control groups. There was no statistically significant difference in the distribution of NUCB2 genotypes among the groups. PMID: 26369257
  23. Nesfatin-1 hormone levels increased significantly in morbidly obese patients following laparoscopic sleeve gastrectomy. PMID: 27049252
  24. serum level elevated in fetal growth retardation PMID: 26043295
  25. Data indicate a positive correlation of nesfatin-1 and a negative correlation of orexin-A with body mass index. PMID: 26831553
  26. 1012C>G polymorphism of NUCB2 is correlated with a reduced risk of developing MetS in a Chinese Han population. PMID: 26624852
  27. In acute myocardial infarction patients, plasma nesfatin-1 levels were negatively correlated with high-sensitivity C-reactive protein, neutrophil% or Gensini scores. PMID: 23806888
  28. nesfatin-1 might have an important role in regulation of food intake and pathogenesis of loss of appetite in children. PMID: 26744860
  29. Nesfatin-1 levels were significantly lower in metabolic syndrome (MS) group compared to non-MS group PMID: 25432328
  30. These results corroborate the suggestion of NUCB2/nesfatin-1 being relevantly involved in the regulation of mood and stress in a sex-specific way PMID: 26143537
  31. Serum nesfatin-1 levels are significantly higher in girls with premature thelarche compared to prepubertal controls. PMID: 25833360
  32. Circulating NUCB2/nesfatin-1 levels correlated positively with perceived anxiety, whereas no association with body mass index or eating disorder symptoms was observed. PMID: 26162003
  33. Our results show that NUCB2 plays an important role in tumorigenesis and progression and is a potential molecular biomarker for the diagnosis and targeted therapy of ccRCC. PMID: 25322808
  34. This investigation indicates a marked association of serum and synovial fluid nesfatin-1 concentrations with osteoarthritis disease severity. PMID: 25872767
  35. This study shows an association of serum nesfatin-1 concentrations and the development and severity of peripheral arterial disease in type 2 diabetes mellitus patients. PMID: 25841171
  36. Plasma IL-6 and TNF-alpha did not significantly change after training, but nesfatin increased significantly only with high-intensity interval training compared with the control group. PMID: 25581765
  37. nesfatin-1 inhibits the growth of adrenocortical H295R cells and promotes apoptosis, potentially via the involvement of Bax, BCL-XL and BCL-2 genes as well as ERK1/2, p38 and JNK1/2 signalling cascades PMID: 25869615
  38. Data suggest that plasma nesfatin-1/NUCB2 levels are up-regulated in pregnant women recently diagnosed with polycystic ovary syndrome (PCOS) as compared to healthy women; many of those with PCOS were also overweight and exhibited insulin resistance. PMID: 24920281
  39. Data indicate that patients with severe preeclampsia showed significantly decreased levels of serum nesfatin-1 compared with those with mild preeclampsia. PMID: 24857385
  40. there was no significant increase in postprandial level of nesfatin-1. This suggests that oral glucose load in obese children may not be sufficient for nesfatin-1 response and that nesfatin-1 may not have an effect as short-term regulator of food intake. PMID: 24825087
  41. NUCB2/nesfatin-1 and visfatin are novel factors associated with systemic inflammation in COPD; visfatin may mediate impaired pulmonary diffusing capacity PMID: 24891763
  42. Serum nesfatin-1 levels were measured in patients with subclinical or overt hyperthyroidism to see whether nesfatin-1 is involved in the regulation of appetite and body weight in hyperthyroidism. No significant difference from normal controls was found. PMID: 25068306
  43. Increased nucleobindin-2 (NUCB2) transcriptional activity links the regulation of insulin sensitivity in Type 2 diabetes mellitus. PMID: 23765387
  44. Nesfatin-1 might have an important role in regulation of food intake and pathogenesis of insulin resistance in obese children and young adolescents. PMID: 24333832
  45. NUCB2 protein expression showed a strong association with the potencies of BCR and progression of PCa, and that may be applied as a novel biomarker. PMID: 24422979
  46. Role of NUCB2/Nesfatin-1 in the hypothalamic control of energy homeostasis. PMID: 24048879
  47. In advanced cystic fibrosis and low fat mass, nesfatin-1 plasma levels are significantly increased and inversely correlated with the fat mass. PMID: 23306670
  48. plasma NUCB2/nesfatin-1 levels were altered under conditions of perceived anxiety, stress and depression in obese women. PMID: 23796625
  49. Nesfatin-1 is present in articular tissues and may contribute to the physiopathologic changes in OA. Nesfatin-1, accompanied with hsCRP and IL-18, could have roles in the progression of knee osteoarthritis PMID: 24259949
  50. we demonstrated that high NUCB2 mRNA expression correlated with poor overall survival in patients with prostate cancer PMID: 24092574
  51. Review highlights evidence of changes in circulating levels of NUCB2/nesfatin-1 in disease states, occurrence of NUCB2 polymorphisms and independence of leptin signalling known to be blunted under conditions of chronically increased body weight. PMID: 23980879
  52. study demonstrated cytoplasmic localization of NUCB2/nesfatin-1 peptide in both human and murine chondrocytes. PMID: 24464950
  53. Kaplan-Meier survival analysis showed that patients with high NUCB2 expression have shorter biochemical recurrence (BCR)-free survival time compared to patients with low NUCB2 expression. PMID: 23958433
  54. Data suggest that NUCB2 expression is widespread in including adipose tissue, pancreas, and digestive tract; NUCB2 appears to participate in regulation of energy homeostasis/appetite; genetic polymorphisms in NUCB2 may predispose to obesity. [REVIEW] PMID: 23955480
  55. A statistically significant correlation was found between the serum nesfatin-1 level and supraventricular tachycardia. PMID: 23524986
  56. Nesfatin-1 levels were lower in children with untreated autoimmune thyroid diseases, however, the mechanism is also unknown. PMID: 23739643
  57. Circulating nesfatin-1 may modulate glucose homeostasis during diabetes remission in gastric bypass and sleeve gastrectomy, and participate in regulating body weight in sleeve gastrectomy PMID: 23768444
  58. This study provides a novel regulatory signaling pathway of nesfatin-1-regulated ovarian epithelial carcinoma growth and may contribute to ovarian cancer prevention and therapy, especially in obese patients. PMID: 23764403
  59. Ghrelin and NUCB2/nesfatin-1 are expressed in the same gastric cell and differentially correlated with body mass index in obese subjects. PMID: 23515787
  60. pro-osteogenic activity of NUCB2(1-83) PMID: 23613885
  61. Data suggest that serum nesfatin-1 are significantly lower in obese youth than in healthy control youth; no association was found with insulin resistance in obese subjects. PMID: 23346951
  62. Loss of fat mass may decrease serum nesfatin-1 level in lung cancer patients with weight loss. PMID: 23269222
  63. nesfatin-1 levels are decreased in large for gestational age compared to appropriate for gestational age fetuses. PMID: 23178145
  64. Nesfatin-1 concentrations were reduced in non-alcoholic fatty liver disease. PMID: 22950627
  65. Lower nesfatin-1 concentration may plays a very important role in the development of polycystic ovary syndrome PMID: 22367584
  66. we have identified the first rare genetic variants in the NUCB2 gene in obese individuals (frequency 0.2%), suggesting that NUCB2 might indeed be involved in the regulation of energy homeostasis and food intake. PMID: 23141462
  67. The NUCB2 variant c.1012C>G (Q338E) is associated with childhood adiposity. The GG genotype may be protective against excessive weight gain. PMID: 23266808
  68. We have demonstrated that the Wa antigen is NEFA or nucleobindin-2, which binds specific tRNA species, and is distributed in specific human tissues. PMID: 22270345
  69. Nesfatin-1 is involved in the physiological regulation of intrauterine and postnatal growth and development in small for gestational ages infants. PMID: 23155701
  70. Data suggest that maternal serum levels of nesfatin-1 (derived from NUCB2) are lower in patients with gestational diabetes compared with control pregnant women; however, the cord blood nesfatin-1 levels are similar. PMID: 22203468
  71. the details of nesfatin-1 physiology ought to be clarified, and it may be considered suitable in the future, as a potential drug in the pharmacotherapy of obesity--{REVIEW} PMID: 22225987
  72. nesfatin-1 levels may be regulated by nutrition status and response to starvation. PMID: 20937336
  73. This study demonistrated thatsex difference in NUCB2 dynamic expressions in the midbrain of drug-free depressed suicide victims. PMID: 21803054
  74. Human islet NUCB2 mRNA was reduced in T2D subjects but upregulated after culture in glucolipotoxic conditions. Furthermore, a positive correlation between NUCB2 and glucagon and insulin gene expression, as well as insulin secretory capacity, was evident. PMID: 22108805
  75. NUCB2 may operate directly at the testicular level to link energy homeostasis, puberty onset, and gonadal function PMID: 22334726
  76. Plasma nesfatin-1 concentrations were found to be elevated in subjects with both impaired glucose tolerance and newly diagnosed T2 diabetes mellitus. PMID: 22020667
  77. nesfatin-1 levels did not differ between hemodialysis patients and healthy subjects and negatively correlated with protein intake PMID: 22036920
  78. High NUCB2 is associated with recurrence and metastasis in breast carcinoma. PMID: 21988594
  79. polymorphisms in the NUCB2 gene could play an important role in the protection against the development of obesity in male subjects PMID: 21459029
  80. Increased serum prolactin and nesfatin-1 concentrations, decreased ghrelin concentrations could be used as markers to identify patients that have suffered a recent epileptic seizure or other paroxysmal event (psychogenic). PMID: 21554911
  81. nucb2 is the direct target gene of AML1 and AML1-ETO, the transcription regulation of AML1, AML1-ETO on nucb2 is carried out via repressing its promoter activity. PMID: 20030931
  82. Potential weight-reducing actions of nesfatin-1/NUCB-2 treatment. PMID: 21252251
  83. Apelins and nesfatin-1 concentrations were higher in mature milk than in colostrum (P<0.05). The concentration of apelins, ghrelins and nesfatin-1 in serum and milk in gestational diabetic lactating women was lower than in control samples PMID: 20813143
  84. lack of nesfatin-1 response to the exercise protocols may be partially due to the fasting condition PMID: 20625762
  85. low ghrelin and especially the dramatically elevated nesfatin-1 levels might contribute to the pathophyisology of epilepsy[Nesfatin-1] PMID: 19262995
  86. Data report that nesfatin-1 is a novel depot specific adipokine preferentially produced by sc tissue, with obesity- and food deprivation-regulated expression. PMID: 20427481
  87. The release of NUCB2 from isolated islets was significantly elevated following glucose challenge. PMID: 20032201
  88. NUCB2-ARTS-1 complexes play an important role in mediating tumor necrosis factor receptor 1 release to the extracellular compartment. PMID: 16407280
  89. NUCB2 may be implicated in gastric secretion by establishing an agonist-releasable Ca2+ store in ER or Golgi apparatus, signalling via heterotrimeric Galpha proteins and/or mediating the exocytosis of the secretory granules. PMID: 19351608
  90. Calcium-dependent ARTS-1-nucleobindin 2 complexes associate with TNFR1 prior to the commitment of TNFR1 to pathways that result in the constitutive release of TNFR1 exosome-like vesicles or the inducible proteolytic cleavage of TNFR1 ectodomains. PMID: 16407280

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Subcellular Location Golgi apparatus, Membrane, Peripheral membrane protein, Cytoplasm, Secreted, Endoplasmic reticulum, Nucleus envelope
Protein Families Nucleobindin family
Tissue Specificity Predominantly expressed in spleen, testis and normal stomach.
Database Links

HGNC: 8044

OMIM: 608020

KEGG: hsa:4925

STRING: 9606.ENSP00000436455

UniGene: Hs.654599

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